2017-03-032017-03-032016-03CARVALHO, Josiwander Miranda et al. Phytosterols isolated from endophytic fungus Colletotrichum gloeosporioides(Melanconiaceae). Acta Amazonica, Manaus, v. 46, n. 1, p. 69-72, Jan./Mar. 2016. DOI: http://dx.doi.org/10.1590/1809-4392201500072. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/7780. Acesso em:.1809-4392https://repositorio.ufpa.br/handle/2011/7780Endophytic fungi are fungi that colonize internal tissues of plants. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, the aim this study was the isolation and structural identification of sitosterol (1), stigmasterol (2), sitostenone (3), squalene (4), ergosterol (5) and ergosterol peroxide (6) from fungus Colletotrichum gloeosporioides isolated as endophytic from Virola michelli, a typical Amazon plant, used in folk medicine against skin infection. Compounds were isolated by chromatography column on silica and identified by 1 H and 13C NMR and MS. The presence of phytosterols in fungi is rare and this is the first report of the isolation of the phytosterols sitosterol, stigmasterol and sitostenone from the genus Colletotrichum.Acesso AbertoFungo endofíticoMetabólitosPlantasFitosteróisFitoquímicaSterolsSecondary metabolitesAmazon fungiArtigo de Periódicodx.doi.org/10.1590/1809-4392201500072