2015-04-062015-04-062014-09SOUZA, Joanne M. M.; BERKOV, Strahill; SANTOS, Alberdan S. Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 86, n. 3, p. 1015-1027, set. 2014. Disponível em: <http://www.scielo.br/pdf/aabc/v86n3/0001-3765-aabc-86-03-1015.pdf>. Acesso em: 01 abr. 2015. <http://dx.doi.org/10.1590/0001-3765201420130098>.0001-3765https://repositorio.ufpa.br/handle/2011/6522In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m–2 s–1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and glucose (1.5 %; w/v). Lipidomics techniques were applied in hexane fraction showing higher concentrations of the steroids β-sitosterol (3.53 mg/100 g dc–dry cells), and fatty acids, especially 2-hydroxy-tetracosanoic acid (0.34 mg/100 g dc), eicosanoic acid (86.25 mg/100 g dc), stearic acid (420.83 mg/100 g dc), tetradecanoic acid (10.74 mg/100 g dc) and linoleic acid (100.61 mg/100 g dc). The lipid profile of callus versus that found in the roots of wild plant is described in this work.engAcesso AbertoPlantas medicinaisBoerhaavia paniculataCalogêneseLipídeosPerfil lipídeosPará - EstadoAmazônia brasileiraArtigo de Periódico