Estudo de misturas de enzimas (complexo celulásico, complexo enzimático, xilanase, β-glucanase e xilanase, β-glucosidase e Glucoamilase) na bioconversão do bagaço da cana-de-açúcar em etanol

2017-02-142017-02-142015MOREIRA, Rosiane Fernandes. Estudo de misturas de enzimas (complexo celulásico, complexo enzimático, xilanase, β-glucanase e xilanase, β-glucosidase e Glucoamilase) na bioconversão do bagaço da cana-de-açúcar em etanol. Orientador: Nélio Teixeira Machado. 2015. 100 f. Dissertação (Mestrado em Engenharia Química) – Instituto de Tecnologia, Universidade Federal do Pará, Belém, 2015. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/7678. Acesso em:.https://repositorio.ufpa.br/handle/2011/7678This work, it was proposed to evaluate mixtures of commercial enzymes by supplier Novozymes A / S. The enzymes used in this work were: celulase complex, xylanase, β-glucosidase, enzymático complex, xylanase and β-glucanase and glucoamylase in the glucose production from sugarcane bagasse subjected to treatment with alkali hydroxide solution sodium at room temperature, 70 ° C, 90 ° C and 120 ° C. The BCA yield on a dry basis after treatment with NaOH solution 6 (w / v) were 30.64% ± 1.395 (PACTA), 44.00% ± 1.787 (PAC70), 65.91% ± 1.096 (PAC90), and 95.25% ± 1.461 (CAP 120), respectively. The ash content for the BCA were 2.05% ± 0.027 (PACTA), 0.62% ± 0.013 (PAC70), 0.48% ± 0.007 (PAC90) and 0.18% ± 0.008 (PAC120). The lignin contents were 20.67 ± 0.603 (PACTA), 13.03 ± 0.711 (PAC70), 6.05 ± 0.196 (PAC90) and 5.49 ± 0.151 (PAC120). The results suggest that the conversion rates of cellulosic waste into glucose are strongly dependent on temperature in the alkaline pulping process. The kinetic parameters obtained in kinetic adjustments enzymatic hydrolysis of the BCA for PACTA, PAC70, PAC90 and PAC120 were: Vmax (g/h) equal to 7.20; 5.12; 4.54 and 0.87 respectively; Km (g) equal to 3.6; 2.56; 2.27 and 2.56 respectively; Kcat (h) equal to 1.44; 1.02; 0.91 and 0.17 respectively; Km/Vmax equal to 0.5 for all samples and Kcat/Km of 0.4 for all samples.Acesso AbertoCinética enzimáticaHidróliseFermentaçãoCeluloseProcessos químicosLigninaPolpação alcalinaNovozymes A/SCana-de-açúcarEnzimasEtanolBioconversãoEnzymatic kineticsHydrolysisFermentationCelluloseChemical processesAlkaline pulpSugar caneEnzymesBioconversionEstudo de misturas de enzimas (complexo celulásico, complexo enzimático, xilanase, β-glucanase e xilanase, β-glucosidase e Glucoamilase) na bioconversão do bagaço da cana-de-açúcar em etanolStudy of enzyme mixtures (cellulase complex, enzyme complex, xylanase, β-glucanase and xylanase, β-glucosidase and Glucoamylase) in the bioconversion of sugarcane bagasse into ethanolDissertaçãoCNPQ::ENGENHARIAS::ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICA