2011-05-042011-05-042007-10Carneiro, T.X. et al. Diversidade genética e eficiência de DNA microssatélites para o controle genealógico da raça Nelore. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, online, v. 59, n. 5, p. 1257-1262, out. 2007. DOI: http://dx.doi.org/10.1590/S0102-09352007000500024. Disponível em: http://www.repositorio.ufpa.br/jspui/handle/2011/2157. Acesso em:.0102-0935http://www.repositorio.ufpa.br/jspui/handle/2011/2157The genetic variability and paternity testing values in Nelore breed were estimated using 11 ISAG/FAO microsatellites. The markers were organized into 4 amplification groups for semi-automated fluorescence genotyping. All markers were highly polymorphic, with an average of 8.2 alleles per locus. With a mean value of 0.48, the observed heterozygosity was lower than the expected for 10 of the loci. A significant deficit of heterozygotes was observed for 9 loci, resulting in a lack of Hardy-Weinberg equilibrium in the studied population. Polymorphism information content values exceeded 0.5 for 10 loci. The power of discrimination was >0.999 and paternity exclusion probabilities when a mother, her offspring and a putative sire are compared or when one or other parental genotype is unavailable for the combined set of markers were, respectively, >0.999 and >0.989. The set of 11 microsatellite markers proved to be an efficient tool for paternity testing in Nelore cattle.Acesso AbertoBovinoGado neloreTeste de paternidadeGenética animalArtigo de Periódico10.1590/S0102-09352007000500024