2014-09-102014-09-102007-04-02FERREIRA, Silvaney Fonseca. Caracterização genética de avestruzes (Struthio camelus) usando marcadores RAPD. 2007. 57 f. Dissertação (Mestrado) - Universidade Federal do Pará, Núcleo de Ciências Agrárias e Desenvolvimento Rural, Empresa Brasileira de Pesquisa Agropecuária, Universidade Federal Rural da Amazônia, Belém, 2007. Programa de Pós-Graduação em Ciência Animal.https://repositorio.ufpa.br/handle/2011/5669The objective of this work was to evaluate the genetic variability of ostrich populations (Struthio camelus) through RAPD markers (Random Amplified Polymorphic DNA). 121 samples of individuals were used from Pará, Maranhão, Tocantins and Minas Gerais States. The genomic DNA was extracted from total blood. Fifteen primers were selected among the 60. The products of the PCR were visualized in agarose gel 1.5% and, a binary matrix was generated considering the presence (1) of a amplified fragment and its absence (0). The ideal number of polymorphic bands was estimated through the bootstrap analysis using the GQMOL software. The genetic similarity was estimated through the Jaccard coefficient using the NTSYS-PC (Numerical Taxonomy and Multivariate Analysis System) software, version 2.02. The origin of the genetic diversity was quantified by the analysis of molecular variance (AMOVA) using the Arlequin 2,0 software. The 15 primers generated a total of 109 polymorphic bands and the bootstrap analysis showed that at least 100 bands is the ideal number for sampling the genetic diversity, as determined by the high value of correlation (r=0,99), the low value of the squared deviation sum (1,25), and the low stress (0,05). The results suggest that the studied populations are from the same origin. Management measures must be adopted in these breeding, even using other molecular markers in the way to amplify the genetic variability and the conservation of this important genetic resource. RAPD.The bootstrap analysis showed that from 100 bands the work already becomes more trustworthy, a time that the magnitude of the correlation was well next to the maximum value (r=0,99), as also the addition of squares of shunting lines (SQd) reached low value 1,25 and the value of it estresse (e) was of 0,05. In the analysis between pairs of groups, it was verified that the greater and minor similarity are in lathe, respectively, of 0,86 and 0,00. In that it says respect to the distribution of frequency of the similarities gotten between the 5,644 pairs formed in the genetic matrix, it can be verified that 32,69 % of the pairs had been enclosed in the classrooms with similarities varying of 0,01 the 0,10. One notices that the biggest percentage (85,59%) of the pairs was distributed in the three first classrooms of the extremities and that the minority of them (14,41%) presented similarities varying of 0,21 the 1,00. The test of Mantel showed correlation of 0,81 and the dendrograma generated 67 groups delimited for the Sm that was of 0,49. The biggest 0,86 similarity was of and the minor of 0,06. The relative data to the analysis of molecular variance had shown that the percentage of genetic variation between origins was low and significant (24,03%, p < 0,0001), evidencing that great part of the variation meets inside of the populations (75,97 %). markers RAPD they had been efficient in the characterization of the genetic similarity.porAcesso AbertoMelhoramento genéticoAvestruzStruthio camelusMarcadores molecularesMarcadores RAPDDNAMaranhão - EstadoTocantins - EstadoPará - EstadoAmazônia brasileiraMinas Gerais - EstadoDissertaçãoCNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMAL