2012-04-042012-04-042011-12-29GARCEZ, Daniela Rosa. Análise comportamental e histológica de um modelo animal da doença de Parkinson em camundongos suíços. Orientadora: Elizabeth Sumi Yamada. 2011. 49 f. Dissertação (Mestrado em Neurociências) - Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, 2011. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/2596. Acesso em:.https://repositorio.ufpa.br/handle/2011/2596Parkinson’s disease (PD) is one of the most common aging-related neurodegenerative diseases, having a clinical presentation featuring classic motor symptoms related to the degeneration of dopaminergic neurons of the substantia nigra pars compacta (SNpc) and dopamine decr ease in the striatum. Animal models of PD are important tools employed by researcher aiming a better understading of pathophysiologic disease mechanisms and for evaluation of potential therapeutic interventions. Such models must mimic some aspect of the disease as for instances, the degeneration of nigral dopaminergic neurons. In this context, the PD model induced by the injection of the neurotoxina 6-hydroxydopamine (6-OHDA) has been widely established in rats but a better characterization in diferent mice strain is lacking, concerning both behavioral changes and the lesion in nigrostriatal system. Such characterization is important so that this model can be reliably used for investigations of therapeutic interventions. The goal of the present study was to improve the characterization of the unilateral 6-OHDA PD model using Swiss mice, through the evaluation of behavioral changes and the effects on the SNpc dopaminergic neurons. In this investigation we have used a single unilateral intraestriatal injection of 6-OHDA, in two different toxin concentrations: 10 µg/2µl e 20 µg/2 µl. Our results have demonstrated that both 6-OHDA concentrations used provoked severe loss of nigral dopaminergic neurons, amounting to 74,5% e 89,5% respectively. This neuronal loss was highly correlated to the apomorphine-induced rotational behavior but not to the ambulation assessed in the open field test. Therefore, intraestriatal injection of 10 µg/2µl or 20 µg/2µl of 6-OHDA, using Swiss mice, reproduce an effective unilateral 6-OHDA PD model that can be reliably employed in experiments aiming to investigate neuroprotective, cellular and/or pharmacological therapies for PD.porAcesso AbertoDoença de ParkinsonDoenças neurodegenerativasHidroxidopaminasNeurotoxidadeDissertaçãoCNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA GERAL::NEUROFISIOLOGIA