2013-08-082013-08-082013-04SILVA, Sandro P., et al. Comparação das técnicas de ELISA indireto e Imunofluorescência indireta na detecção de anticorpos anti-Neospora caninum em búfalas (Bubalus bubalis). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 33, n. 4, p. 431-434, abr. 2013. Disponível em: <http://www.scielo.br/pdf/pvb/v33n4/v33n4a03.pdf>. Acesso em: 30 jul. 2013. <http://dx.doi.org/10.1590/S0100-736X2013000400003>.0100-736X1678-5150https://repositorio.ufpa.br/handle/2011/4096To compare two serologic tests for detection of antibodies against Neospora caninum in sera from buffaloes, samples were collected from 288 buffaloes of 2 to 10 years of age. To identify the presence of IgG, anti-N. caninum was used for the indirect immunofluorescence assay (IFAT), with title 200 as the cutoff, and for the immunoenzymatic test (indirect ELISA), considering as positive samples with ratio S/P >0.5. There were 153 (53.12%) animals positive for N. caninum by IFAT, whilst 50 (17.36%) animals were reactive in ELISA. The presence of antibodies anti-N. caninum demonstrates that the parasite is circulating among buffaloes raised in Pará State, Brazil. ELISA and IFAT tests could be used to diagnose immunoglobulins against this agent. However there was a weak correlation (Kappa = 0.36) between both tests, considering the IFAT as the gold standard.porAcesso AbertoSorologiaReação de Imunofluorescência Indireta (RIFI)Ensaio de imunoadsorção enzimáticaNeosporaBúfaloBubalinosPará - EstadoAmazônia brasileiraArtigo de Periódico