2013-04-182013-04-182006ARAÚJO, Tais Pinheiro de. Caracterização morfológica e antigênica do vírus Juruaçá, isolado de morcego no estado do Pará. 2006. 123 f. Dissertação (Mestrado) - Universidade Federal do Pará, Núcleo de Medicina Tropical, Belém, 2006. Curso de Pós-Graduação em Patologia das Doenças Tropicais.https://repositorio.ufpa.br/handle/2011/3700Juruaçá virus (BE AN 401933) was isolated from pooled organs of an unidentified bat captured during field work in Porto Trombetas, Oriximiná, Pará State, in 1982, and remains unclassified/ungrouped. The aims of this work were to classify Juruaçá virus in a viral taxon taking in account its morphological, physicochemical, antigenic and molecular properties, as well as, to describe the pathological alterations. This agent is pathogenic only for infant mice, and the animal’s death occurs with eight days post-inoculation. It’s not related to any of the arthropod borne viruses with which it has been tested by serological tests, such as complement fixation (CF) and hemagglutination inhibition (HI). Positive reactions were only observed with its homologous serum. Juruaçá virus is not sensitive to sodium desoxicolate and can hemagglutinate goose cells at pH 5.75. The virus didn’t show any cytophatic effect and immunofluorescence was negative in Vero and C6/36 cell lines, but it replicates in brain tissue primary culture cell line (astrocytes and microglias), confirmed by immunofluorescent assay (IFA). Culture of neuronal cells appears not to be infected by Juruaçá virus; however, infection of these cells was confirmed by imunohistochemistry. By transmission electron microscopy and negative stain, the virus is a spherical particle, with mean diameter of 23-30nm. Pathological alterations were observed mainly in the central nervous system of newborn mice experimentally infected with Juruaçá virus. The molecular results of RT-PCR suggest that Juruaçá virus is a possible new virus belonging to the family Picornaviridae, genus Enterovirus.porAcesso AbertoVirologiaVírus JuruaçáPorto Trombetas - PAOriximiná - PAPará - EstadoAmazônia brasileiraDissertaçãoCNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIACNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS