2013-05-272013-05-272011LIMA, Edna Porfírio de. Estudo clínico, morfológico e imuno-histoquímico de série de casos de tuberculose pleural e ganglionar. 2011. 69 f. Dissertação (Mestrado) - Universidade Federal do Pará, Núcleo de Medicina Tropical, Belém, 2011. Programa de Pós-Graduação em Doenças Tropicais.https://repositorio.ufpa.br/handle/2011/3903The difficulty in definitive diagnosis of extra-pulmonary tuberculosis persists, mainly due to poor solutions available from conventional methods for detection of Mycobacterium tuberculosis. This study aimed to evaluate the contribution of immunohistochemical (IHC) for detection of Mycobacterium spp, in cases of pleural and lymph node tuberculosis with negative staining, as well as investigate some clinical, laboratory and morphological aspects of the disease. To obtain this sample was made in the pursuit of cases through surveillance (NVE) and Division of Medical Archives and Statistics (DAME), University Hospital João de Barros Barreto (HUJBB) and the Department of Pathology, University of Pará (UFPA), selecting those who had performed the histopathological examination for diagnosis of the case. Fifty patients were included, twenty-five with presumptive diagnosis of pleural tuberculosis and twenty-five of lymph node tuberculosis. To obtain the clinical and laboratory data were reviewed their medical records, and to confirm the morphological aspects review was performed of all selected slides. Thereafter, each sample was subjected to IHC with polyclonal Mycobacterium bovis BCG. It was found in the investigated group, more often male, whose average age was 33.8 years (SD: 14.1) with the majority coming from the city of Belem, Pará and education level of seven or fewer years of schooling. Constitutional symptoms more frequent in the whole group were fever and weight loss. In patients with pleural tuberculosis, the most frequent specific symptoms were cough, chest pain and dyspnea, and in those with lymph node involvement of the cervical alone was more frequent. Infection with human immunodeficiency virus (HIV) or Acquired Immune Deficiency Syndrome (AIDS) and alcohol consumption were the risk conditions most frequently associated. In pleural tuberculosis, 20% of cases presenting with pleural effusion associated with parenchymal injury, and 60% of the pleural fluid was exudate type. While in the lymph nodes in 50% of the cases revealed a parenchymal lesion on chest. This study was marginal participants in the amount of which has been held to direct research and culture for bacillus acid – resistant (AFB) in various clinical specimens analyzed (pleural fluid, pleural tissue and lymph node, sputum, and broncho-alveolar lavage). The predominant morphological pattern in both forms of the disease was tuberculous granulomas with caseous necrosis, regardless of serologic status for HIV. The IHC technique contributed to the diagnosis of pleural tuberculosis in 21% (4/19) samples of pleural tissue and 37.5% (9/24) of lymph node tissue. A positive immunohistochemical result defines the diagnosis of mycobacterial disease, and when associated with clinical, laboratory and morphological finds become a valuable tool to improve the diagnosis of extra-pulmonary tuberculosis.porAcesso AbertoTuberculose extrapulmonarImunoistoquímicaTuberculose pulmonarTuberculose ganglionarTuberculose pleuralInfecções por mycobacteriumMycobacterium bovisDiagnóstico clínicoBelém - PAPará - EstadoAmazônia brasileiraDissertaçãoCNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVA::SAUDE PUBLICACNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS