2013-04-172013-04-172003PIMENTA, Adriana do Socorro Coelho. O uso da técnica de reação em cadeia da polimerase (PCR) em tempo real em doadores de sangue soropositivos para o anti-HCV. 2003. 61 f. Dissertação (Mestrado) - Universidade Federal do Pará, Núcleo de Medicina Tropical, Belém, 2003. Curso de Pós-Graduação em Doenças Tropicais.https://repositorio.ufpa.br/handle/2011/3699The HCV is a spherical virus that presents a RNA genome with a positive polarity. Actually classified into the flaviridae family, and Hepacivirus genre, presenting a 9.4 Kb constituted by a unique and long open reading frame (ORF) which comprises almost all the genome. It presents two untranslated regions (UTR) at the 5' and 3' extremities. The major polyprotein is clivated in ten minors proteins, resulting in structural and non-structural proteins. This virus shows preferentially the blood transmission and is distributed around the world. The diagnosis has been done accidentally in the most of cases while sorological and molecular screening is done. This work has as the main objective, to compare the imuno-enzematic assay (ELISA) with polimerase chain reaction test (PCR) in the occasion of pre-selection of blood donors. Detection screening by PCR was done in 290 samples that were positive or indeterminate for ELISA assay. The result analysis showed that the samples positive-ELISA/positive- PCR and positive-ELISA/negative-PCR are two different and independent samples (p=0,0006). This difference is supposed to be due a differential immunologic response of the samples that presented positive PCR result. We attended a correlation between DO/cut-off (ELISA) and viral load in PCR as we see in other virus like HIV, however the results appears totally disperse (R2=0,025), confirming the non-correlation between the two testes, ELISA and PCR for detection of virus C.porAcesso AbertoHepacivirusReação em cadeia da polimeraseDoadores de sangueHepatite CBelém - PAPará - EstadoAmazônia brasileiraDissertaçãoCNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::HEMATOLOGIA