2013-04-242013-04-242013-01-17SILVA JUNIOR, Ademir Ferreira da. Intoxicação crônica experimental com alumínio: padrões degenerativos, comportamentais e terapia experimental com magnésio após lesão hipocampal. 2013. 115 f. Tese (Doutorado) - Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, 2013. Programa de Pós-Graduação em Neurociências e Biologia Celular.https://repositorio.ufpa.br/handle/2011/3735Experimental evidence suggests that aluminum is a neurotoxic agent with harmful effects on cognitive processes. In this study, we investigated the behavioral, biochemical and histopathological effects of chronic intoxication with aluminum citrate on the hippocampus of adult rats, in the same time investigated experimental therapy treatment with magnesium for reversing the neuropathological changes. We used 70 male Wistar rats of 200-250 g that were divided in groups as follows: control, sodium citrate (CNa), aluminum citrate (CAl), aluminum citrate + magnesium sulphate (CAl+Mg), sodium citrate + sulfate magnesium (Mg+CNa). The dose used of aluminum citrate was 100 mg/kg and 250 mg/kg of magnesium sulfate. The neurotoxic compound was taken orally for 30 days. The animals were subjected to behavioral tests of open field, Rota Rod, social recognition and the elevated T maze (LTE). Furthermore, aluminum levels in plasma and hippocampus of animals was found by atomic absorption spectrometer graphite furnace (GF AAS), biochemical analysis, histopathology and immunohistochemistry for GFAP. It was found that the CAl group showed increase locomotor activity in the open field test compared to the control group, and CAl+Mg group showed a decrease (P <0.001). In memory tests of LTE and social Recognition, the CAl group showed cognitive deficits in relation to other groups, and CAl+Mg group performed well in the test (P <0.001). Aluminum levels found in the hippocampus of CAl group were considerably higher but in the other groups the levels were below to the detection limit of the equipment. Histopathology and imunistochemistry analysis in CAl group showed decreased in cell density and astrocytic reactivity in layers CA1, CA3 and hilus of the hippocampus. These results suggest that the experimental poisoning with aluminum citrate induce deficits of learning and memory and that the administration of magnesium sulphate may have the ability to minimize the damage caused by metal in the hippocampus of intoxicated animals.porAcesso AbertoMemóriaHipocampo (Cérebro)AprendizagemCitrato de alumínioMagnésioTeseCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA::TOXICOLOGIA