2014-02-132014-02-132004-09-12COSTA, Marcos Rogério Menezes da. Estudo da susceptibilidade à infecção pelo HIV-1 e da progressão da AIDS em associação ao polimorfismo no gene Mbl (Lectina Ligadora de Manose). 2004. 92 f. Dissertação (Mestrado) – Universidade Federal do Pará, Centro de Ciências Biológicas, Belém, 2004. Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários.https://repositorio.ufpa.br/handle/2011/4772The low serum concentration of Mannose-Binding Lectin (MBL) is associated to the presence of variant alleles Mbl-*B, Mbl-*C and Mbl-*D, and it results in an increased susceptibility to recurrent infections. The present study investigated the association between the Mbl gene polymorphism and the susceptibility to HIV-1 infection. A fragment of 349 bp from the exon 1 of the Mbl gene was amplified by PCR and then submitted to RFLP analysis using the endonucleases BanI and MboII, aiming the identification of the variant alleles. The study of 145 seropositive patients and 99 healthy controls showed the presence of alleles Mbl-*A, Mbl-*B and Mbl-*D, with frequencies of 69%, 22% and 9% among patients and 70.2%, 13.6% and 16.2% among healthy controls, respectively. The analysis of the genotype frequencies showed a high prevalence of the genotypes carriers of variant Mbl-*B among patients seropositive as compared to the healthy controls. Furthermore, the genotype B/B was six times more frequent among patients than the observed to the healthy controls (χ2=4.042; p=0.044). The mean viral load was lower in HIV-1 seropositive patient carrying the Mbl-*A allele than those carrying the variant Mbl-*B allele (5,821 copies/mL vs. 52,253 copies/mL; p= 0.05). Furthermore, patients carrying the allele Mbl-*A showed a significant reduction of the viral load (p<0.001), that was not observed among those carrying the variant Mbl-*B (p=0.999). The results suggest the importance of the Mbl gene polymorphism on the clinical evolution of the patients infected by HIV-1 and that the identification of the Mbl genetic profile, among HIV-1 infected patients, may be an important tool to monitor the evolution and the prognosis of diseases.porAcesso AbertoHIV-1Síndrome de Imunodeficiência AdquiridaLectinas de ligação a manosePolimorfismo genéticoDissertaçãoCNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA HUMANA E MEDICACNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIACNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS