2013-04-062013-04-062005-07LAURENTINO, R. V., et al. Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 4, p. 371-376, jul. 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n4/v100n4a06.pdf>. Acesso em: 03 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762005000400006>.1678-80600074-0276https://repositorio.ufpa.br/handle/2011/3643The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies.engAcesso AbertoHIV-1Vírus linfotrópico de células T humanas tipo 1Diagnóstico laboratorialEpidemiologia molecularVírus 2 linfotrópico T humanoBelém - PAMacapá - APPará - EstadoAmapá - EstadoAmazônia brasileiraArtigo de Periódico