2014-11-282014-11-282003-06DOMINGUES, S.F.S. et al. Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 55, n. 3, p. 301-308, jun. 2003. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009&lng=pt&nrm=iso>. Acesso em: 27 nov. 2014. <http://dx.doi.org/10.1590/S0102-09352003000300009>.0102-0935https://repositorio.ufpa.br/handle/2011/6123The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.engAcesso AbertoPrimataMacaco-pregoCebus apellaOvárioFolículo ovarianoIsolamento reprodutivoPará - EstadoAmazônia brasileiraArtigo de Periódico