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metadata.dc.type: Artigo de Periódico
Issue Date: May-2018
metadata.dc.creator: BRITO, Felipe Nunes
VENDRAMIN, Fabiel Spani
LOPES, Cinthia Távora de Albuquerque
COSTA, Mayra Pauline Ribeiro
OHASHI, Otavio Mitio
MAIA, Jose Guilherme Soares
FERREIRA, Lydia Masako
SILVA, Joyce Kelly do Rosário da
MIRANDA, Moysés dos Santos
metadata.dc.description.affiliation: BRITO, F. N.; VENDRAMIN, F. S.; LOPES, C. T. A.; COSTA, M. P. R.; OHASHI, O. M.; SILVA, J. K. R.; MIRANDA, M. S. Universidade Federal do Pará
Title: Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides
Citation: BRITO, Felipe Nunes et al. Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides. Acta Cirúrgica Brasileira, São Paulo, v. 33, n. 5, p. 431-438, May. 2018. DOI: Disponível em: Acesso em:.
Abstract: Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3. Results: Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells. Conclusion: Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages.
Keywords: Stem cells
Adipose tissue
Cell proliferation
Series/Report no.: Acta Cirúrgica Brasileira
ISSN: 1678-2674 Brasil
Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
metadata.dc.publisher.initials: SOBRADPEC
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - FAMED/ICS

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