Please use this identifier to cite or link to this item: http://repositorio.ufpa.br/jspui/handle/2011/3611
metadata.dc.type: Artigo de Periódico
Issue Date: Nov-2008
metadata.dc.creator: DALL'AGNOL, Leonardo Teixeira
MARTINS, Rosimar Neris
VALLINOTO, Antonio Carlos Rosário
RIBEIRO, Karla Tereza Silva
Title: Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon
Citation: DALL'AGNOL, L. T., et al. Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 678-682, nov. 2008. Disponível em: <http://www.scielo.br/pdf/mioc/v103n7/09.pdf>. Acesso em: 01 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762008000700009>.
Abstract: The present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10ºC and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. The largest variation among isolates was observed in the citrate test (Simmons), in which 12 isolates were positive, and in the oxidation/fermentation of sucrose, with six positives isolates. Chloramphenicol, gentamicin and sulfonamides efficiently inhibited bacterial growth. Amplified products of the recA gene were digested with HindII or PstI, which produced three or four restriction fragments patterns, respectively. The combined analysis arranged the isolates into six genospecies. The higher diversity observed in Belém (genotypes C, D, E and F) may be a consequence of intense human occupation, pollution of the aquatic environment or due to the higher diversity of the environments sampled in that region. In conclusion, a high level of genetic and phenotypic diversity was observed, and four new genospecies were described.
Keywords: Ambiente aquático
Chromobacterium violaceum
Variação genética
Amazônia brasileira
ISSN: 0074-0276
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

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