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Title: Investigação de metabólitos secundários de folhas de Derris urucu (Killip et Smith) Macbr. com atividades biológicas
metadata.dc.creator: LÔBO, Lívia Trindade
metadata.dc.contributor.advisor1: ARRUDA, Mara Silvia Pinheiro
Keywords: Estilbenos
Issue Date: 30-Jun-2009
Publisher: Universidade Federal do Pará
Citation: LÔBO, Lívia Trindade. Investigação de metabólitos secundários de folhas de Derris urucu (Killip et Smith) Macbr. com atividades biológicas. 2009. 267 f. Tese (Doutorado) - Universidade Federal do Pará, Instituto de Ciências Exatas e Naturais, Belém, 2009. Programa de Pós-Graduação em Química.
Abstract: Derris urucu, belonging to the family Leguminosae / Fabaceae, is popularly known as “timbó”. The roots of this species are commonly used as pesticides and poison to fish. From Derris genus many phytochemical studies have been reported, the roots being of the most studied part from the plants of this genus. The name “timbó” is most general for the species Derris urucu and Derris nicou, which are the species that produce, in their roots, rotenoids such as rotenone and deguelin, from which derives the importance of these plants. The extracts, and the substances isolated from this genus are responsible for a wide range of biological activities, mainly the insecticidal activity. From the ethanolic extract of leaves of D. urucu, twelve compounds were isolated and purified by High Performance Liquid Chromatography: five stilbenes, six dihydroflavonols and one flavanone. The structural identification was based on the mass, 1H and 13C NMR spectrometric analysis and two dimensional NMR, beyond comparison with literature data . The ethanolic extract of leaves of D. urucu was submitted to bioassays to avaluate the allelophatic potential, presenting relevant percentage of inhibition of seed germination and development of weeds in pastures. Aiming to detect the substances responsible for allelophatic activity, three stilbenes and three dihydroflavonols were selected and submitted to bioassays of inhibition of germination and development of the radicle and hypocotyl of weeds. The allelophatic tests were performed with the substances isolated and with the combination of them, aiming to assess the synergism between them, but the magnitude of inhibition observed was very low. By other side, when the substances were tested in mixtures there was a significant increase in the percentage of inhibition, so these substances in mixtures, can be considered promising for future studies involving allelophatic activity. An other test was carried out with the isolated compounds. Three stilbenes and five dihydroflavonols were evaluated for DPPH radical scavenging activity (antioxidant activity). In this bioassay, was not observed a significant antioxidant activity, justified by the analysis in the structures of the substances tested.
Appears in Collections:Teses em Química (Doutorado) - PPGQ/ICEN

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