Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/4110
metadata.dc.type: Artigo de Periódico
Issue Date: Apr-2002
metadata.dc.creator: MOLNÁR, László
MOLNÁR, Lászlóné Éva
NUNES, Emilia do Socorro Conceição de Lima
DIAS, Hilma Lúcia Tavares
Title: Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
Citation: MOLNÁR, László, et al. Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 22, n. 2, p. 41-44, abr. 2002. Disponível em: <http://www.scielo.br/pdf/pvb/v22n2/10691.pdf>. Acesso em: 30 jul. 2013. <http://dx.doi.org/10.1590/S0100-736X2002000200002>.
Abstract: Four hundred and forty buffalo sera, selected from about 1,200 blood samples of another study, were examined. The samples were tested by six serological methods: two of agglutination, two of indirect ELISA and two of competitive ELISA. To determine the relative sensitivity and specificity of different tests, animals with a positive result to competitive ELISA of the FAO/IAEA were considered as infected. The relative sensitivity of competitive ELISA, indirect ELISA with conjugate anti-bovine light chain monoclonal antibody labelled with HRPO, indirect ELISA with anti-bovine IgG conjugate, rose Bengal test and rapid slide agglutination test was 100%, 98.57%, 97.14%, 91.42% and 79.28%, and the relative specificity 99.33%, 97.33%, 95.66%, 94.00% and 86.33%, respectively. The value of the different serological tests for the diagnosis of brucellosis is discussed.
Keywords: Brucelose em animais
Bubalinos
Ensaio de imunoadsorção enzimática
Aglutinação
Reação de Imunofluorescência Indireta (RIFI)
ISSN: 1678-5150
0100-736X
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - INEAF

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