Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/4882
Title: Infecção experimental em hamster dourados (Mesocricetus auratus) e caracterização genética parcial de cinco Phlebovirus (Bunyaviridae) do Complexo Candiru isolados na Amazônia brasileira
metadata.dc.creator: NUNES NETO, Joaquim Pinto
metadata.dc.contributor.advisor1: VASCONCELOS, Pedro Fernando da Costa
Keywords: Arbovírus
Flebovírus
Mesocricetus
Bunyaviridae
Amazônia brasileira
Issue Date: 7-Dec-2007
Publisher: Universidade Federal do Pará
Citation: NUNES NETO, Joaquim Pinto. Infecção experimental em hamster dourados (Mesocricetus auratus) e caracterização genética parcial de cinco Phlebovirus (Bunyaviridae) do Complexo Candiru isolados na Amazônia brasileira. 2007. 122 f. Dissertação (Mestrado) – Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, 2007. Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários.
Abstract: The phleboviruses (family Bunyaviridae, genus Phlebovirus) are of considerable public health importance, due to their association with human diseases causing different clinical syndromes. In the Brazilian Amazon region, 23 phlebovirus were presently isolated, four of them have been isolated from humans: Alenquer virus, Candiru virus, Morumbi virus, and Serra Norte virus. These and Itaituba virus, isolated from Didelphis marsupialis belong to the Candiru Complex (Candiru group) and were used for studies on genetic characterization and experimental infection using gold hamsters (Mesocricetus auratus) as animal model. Hamsters showed to be susceptible to infection by these viruses; however signs of disease were not observed. The histophatological analysis demonstrated damages in the liver, kidneys, lung, and central nervous system. More intense lesions were observed in the liver which were characterized by the presence of viral antigens detected by immunohistochemistry assay. The analysis of the partial SRNA and MRNA nucleotide sequences obtained for the five studied phleboviruses showed more similarity among them than with other members of the genus Phlebovirus.More over, is was demonstrated that these phleboviruses were more genetically related to Punta Toro virus. Phylogenetically, regardless of the genome segment analyzed, the five phleboviruses constitute a monophyletic group. In addition the analysis by maximum likelihood method demonstrated different evolutionary origins for the RNA segments, suggesting the occurrence of natural genetic reassortment among the five Amazonian phleboviruses.
URI: http://repositorio.ufpa.br/jspui/handle/2011/4882
Appears in Collections:Dissertações em Biologia de Agentes Infecciosos e Parasitários (Mestrado) - PPGBAIP/ICB

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