Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/4903
Title: Participação das citocinas Interleucina-12, Interferon-γ, Interleucina-4 e Interleucina-10 e investigação de polimorfismos nos genes do INF-γ (IFNG+874) e da IL-10 (IL10-1082) na malária causada por Plasmodium vivax
metadata.dc.creator: MEDINA, Tiago da Silva
metadata.dc.contributor.advisor1: CUNHA, Maristela Gomes da
Keywords: Doenças transmissíveis
Malária
Plasmodium vivax
Citocinas
Interleucina
Interferon
Polimorfismo genético
Pará - Estado
Amazônia brasileira
Issue Date: 5-Oct-2009
Publisher: Universidade Federal do Pará
Citation: MEDINA, Tiago da Silva. Participação das citocinas Interleucina-12, Interferon-γ, Interleucina-4 e Interleucina-10 e investigação de polimorfismos nos genes do INF-γ (IFNG+874) e da IL-10 (IL10-1082) na malária causada por Plasmodium vivax. 2009. 116 f. Dissertação (Mestrado) – Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, 2009. Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários.
Abstract: The malaria immune response is complex, and the activation and regulation mechanisms of the efector and memory T cells remain unclear. In this study, we determined seric levels of Interferon-γ (IFN-γ), Interleukin 10 (IL-10), Interleukin-4 (IL-4) and Interleukin-12 (IL-12) in individuals infected by Plasmodium vivax. We investigated polymorphisms in IFN-γ (IFNG+874) and IL-10 (IL10-1082) genes and their association with seric levels and parasite density. Concentrations of IFN-γ, IL-10, IL-4 and IL-12 were detected by ELISA, and IFNG+874 and IL10- 1082 polymorphisms genotyping were investigated by allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) and PCR-RFLP (restriction fragment length polymorphism) methods, respectively. We detected increased levels of IFN-γ and IL-10 in individuals infected by P. vivax. IFN-γ levels were higher in individuals who were infected for the first time, but there were no association with parasite elimination. Increased levels of IL-10 were associated with higher parasite densities. We did not detect IL-4 and IL-12 seric levels. The frequencies of AA, AT, TT genotypes of IFNG+874 were 0,51, 0,39 e 0,10, respectively. The frequencies of AA, AG, GG genotypes of IL10-1082 were 0,49, 0,43 e 0,08, respectively. We also showed that IFG+874 polymorphism is associated with decreased levels of IFN-γ. The results show a Th1-driven immune response which can be regulated by IL-10 in naturally P.vivax-infected individuals.
URI: http://repositorio.ufpa.br/jspui/handle/2011/4903
Appears in Collections:Dissertações em Biologia de Agentes Infecciosos e Parasitários (Mestrado) - PPGBAIP/ICB

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