Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/5178
metadata.dc.type: Artigo de Periódico
Issue Date: Aug-2010
metadata.dc.creator: RIBEIRO, Helem Ferreira
ALCÂNTARA, Diego Di Felipe Ávila
MATOS, Leomá Albuquerque
SOUSA, João Marcelo de Castro e
LEAL, Mariana Ferreira
SMITH, Marília de Arruda Cardoso
RODRÍGUEZ BURBANO, Rommel Mario
BAHIA, Marcelo de Oliveira
Title: Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line
Citation: RIBEIRO, H.F. et al. Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 43, n. 8, p. 717-721, ago. 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n8/307.pdf>. Acesso em: 24 fev. 2014. <http://dx.doi.org/10.1590/S0100-879X2010007500068>.
Abstract: Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and also to test new therapeutic approaches. The present study characterized cytogenetically PG100, the first commercially available gastric cancer cell line derived from a Brazilian patient who had a gastric adenocarcinoma, using GTG banding and fluorescent in situ hybridization to determine MYC amplification. Twenty metaphases were karyotyped; 19 (95%) of them presented chromosome 8 trisomy, where the MYC gene is located, and 17 (85%) presented a deletion in the 17p region, where the TP53 is located. These are common findings for gastric carcinomas, validating PG100 as an experimental model for this neoplasia. Eighty-six percent of 200 cells analyzed by fluorescent in situ hybridization presented MYC overexpression. Less frequent findings, such as 5p deletions and trisomy 16, open new perspectives for the study of this tumor.
Keywords: Carcinogênese
Cromossomos humanos
Câncer gástrico
Oncogenes
Anormalidades cromossômicas
Carcinoma gástrico
Alterações cromossômicas
Alterações genéticas
ISSN: 1414-431X
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

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