Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/5220
Title: Eletroejaculação em caititus (Tayassu tajacu): características seminais pré e pós-refrigeração
metadata.dc.creator: KAHWAGE, Priscila Reis
metadata.dc.contributor.advisor1: GARCIA, Alexandre Rossetto
Keywords: Suíno
Caititu
Tayassu tajacu
Reprodução (Biologia)
Eletroejaculação
Sêmen
Criopreservação de orgãos, tecidos, etc.
Perfil seminal
Animal em cativeiro
Belém - PA
Pará - Estado
Amazônia brasileira
Issue Date: 20-May-2009
Publisher: Universidade Federal do Pará
Empresa Brasileira de Pesquisa Agropecuária
Universidade Federal Rural da Amazônia
Citation: KAHWAGE, Priscila Reis. Eletroejaculação em caititus (Tayassu tajacu): características seminais pré e pós-refrigeração. 2009. 75 f. Dissertação (Mestrado) - Universidade Federal do Pará, Núcleo de Estudos em Ciência Animal, Empresa Brasileira de Pesquisa Agropecuária, Universidade Federal Rural da Amazônia, Belém, 2009. Curso de Mestrado em Ciência Animal.
Abstract: Studies concerning reproductive biology of collared peccary (Tayassu tajacu) are worthy to improve creation system. The aims of this research were testing electroejaculation as safety method to collect semen; describing the seminal profile along the year; and testing the capacity for spermatozoa storage under refrigeration. Eleven adults males (76.8±37.8 months, 19.5±2.7 kg) were selected for the measurements of testicular length and width. Eight adults males were used to semen collection (45±13 stimuli, 12 volt, 3 seconds; and resting pulse of 3 seconds). Semen samples were evaluated for physic characteristics (appearance, color, volume, sperm concentration, pH, sperm motility and vigor), and morphological characteristics (sperm viability and sperm defects). Semen samples were split and diluted in two different extenders: BTS (short-term storage) or X-Cell ® (long-term storage). Diluted semen samples were maintained under refrigeration at 17ºC. The samples were evaluated immediately after dilution (T0), after 24 hours (T24) and 48 hours (T48) of cooling. Animals presented 3.8±0.4 cm on left testicles length, 2.6±0.3 cm on left testicles width and left testicles consistency of 2.3±0.2; right testicles presented 3.8±0.5 cm length, 2.7±0.3 cm width and 2.3±0.2 for consistency. The success rate obtained with electroejaculation was 75.21%. The seminal features observed were: volume 0.81±0.86 mL, concentration 137.44±153 x 106 sptz/mL, pH 7.92±0.73, motility 52.66±28.79%, vigor 2.2±0.8, viability 55.84±28.55%, primary abnormalities 22.87±12.93%, secondary abnormalities 9.11±5.88% and total abnormalities 31.52±13.81%. There were no significant changes in production and quality semen along the months of the year. There was no significant difference between extenders tested concerning to maintenance capacity of seminal parameters during the storage period of storage; however, significant influence of storage time was observed (P<0.05). Diluted semen in BTS presented at T0, T24 and T48, average sperm motility of 56.1±17.6%, 17.9±23.3% and 7.7±19.6%, the average sperm viability was 60.2±17.2%, 27.9±23.8% and 11.7±20.2%, and the average total defects were 28.1±10.8%, 37.5±12.1% and 41.28±11.4%, respectively. Semen samples diluted in X-Cell® presented at T0, T24 and T48 average motility of 54.2±22.1%, 27.0±28.0% and 14.0±24.2%, average viability of 57.8±21.7%, 33.0±26.9% and 19.9±25.6% and total defects were 30.8±11.2%, 41.2±13.4% and 44.2±14.4%. Thus, electroejaculation is an effective and safe method to obtain seminal samples from collared peccary, the semen of collared peccary has potential for storage and can be maintained under 17º C up to 24 hours.
URI: http://repositorio.ufpa.br/jspui/handle/2011/5220
Appears in Collections:Dissertações em Ciência Animal (Mestrado) - PPGCAN/Castanhal

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