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Title: Desenvolvimento de um método para caracterização do extrato hidroetanólico das folhas de Mikania lindleyana DC por Cromatografia Líquida de Alta Eficiência (CLAE)
metadata.dc.creator: OWITI, Alex Oselu
metadata.dc.contributor.advisor1: BARBOSA, Wagner Luiz Ramos
Keywords: Plantas medicinais
Mikania lindleyana
Cromatografia em camada delgada
Cromatografia líquida acoplada com detector de arranjo diodo
Controle de qualidade
Amazônia brasileira
Issue Date: 2011
Publisher: Universidade Federal do Pará
Citation: OWITI, Alex Oselu. Desenvolvimento de um método para caracterização do extrato hidroetanólico das folhas de Mikania lindleyana DC por Cromatografia Líquida de Alta Eficiência (CLAE). 2011. 71 f. Dissertação (Mestrado) - Universidade Federal do Pará, Instituto de Ciências da Saúde, Belém, 2011. Programa de Pós-Graduação em Ciências Farmacêuticas.
Abstract: Mikania lindleyana DC (Asteraceae), vernacular “Sucuriju”, is a creeping, shrubby, perennial, evergreen plant, growing in the Amazon region where it is used as diuretic, anti-hypertensive, anti-inflammatory and anti-ulcerous medicine. The main aim of this study was to develop a method for characterization of hydro-ethanolic extract (tincture) from the leaves of M. lindleyana DC, using UV and High performance Liquid Chromatography (HPLC). The hydro-ethanolic extract (tincture) was prepared based on Brazilian Pharmacopeia V, 2010 and its dry residue was submitted to phytochemical analyses, Thin Layer Chromatography (TLC) and High Performance Liquid chromatography (HPLC). Among the substances observed in the phytochemical analysis was coumarin, alkaloids, amino acids, reducing sugars, phenols, tannins, steroids, terpenes, saponins and organic acids. TLC test was done by applying hexanic, chloroformic and ethyl acetate Fractions together with Crude hydro-ethanolic extract and aqueous solution of coumarin Aldrich® 0,1mg/ml on the same plate using toluene/dichloromethane/acetone (45:25:30) as the eluent system. The chromatogram was then sprayed with an alcoholic solution of KOH 5%, and then observed under UV light at 254nm and 365 nm, revealing a light green band at an RF-0.61 for chloroformic fraction, crude hydro-ethanolic extract and for coumarin samples. The HPLC analysis was conducted using methanol/water 53:47 as the eluent system. The chloroformic fraction(CL), coumarin and the crude hydro-ethanolic extract (CHE) showed peaks at Retention time around 6.00minutes and spectra with maximum absorptions between 270nm and 300nm.This clearly demonstrate the presence of coumarins in the samples (CL= 0.209mg/mL and CHE= 0.014mg/mL).
Appears in Collections:Dissertações em Ciências Farmacêuticas (Mestrado) - PPGCF/ICS

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