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Title: Dinâmica celular e microbiológica do leite de ovelhas Santa Inês acompanhadas durante a lactação
Other Titles: Cellular dynamics and microbiological of milk of Santa Inês ewes accompanied during lactation
metadata.dc.creator: GUARANÁ, Eduardo Levi de Sousa
SANTOS, Rogério Adriano dos
CAMPOS, Anne Grace Silva Siqueira
SILVA, Natália da Silva e
AFONSO, José Augusto Bastos
MENDONÇA, Carla Lopes de
Keywords: Ovinos
California mastite
Staphylococcus spp.
Testes de sensibilidade bacteriana
Infecção intramamária
Células somáticas
Isolamento de bactérias
Issue Date: Oct-2011
Citation: GUARANA, Eduardo Levi de Sousa et al. Dinâmica celular e microbiológica do leite de ovelhas Santa Inês acompanhadas durante a lactação. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 31, n. 10, p. 851-858, out. 2011. Disponível em: <>. Acesso em: 27 jun. 2014. <>.
Abstract: The aim of this study was to evaluate the dynamics of intramammary infection in ewes by means of clinical examination, direct somatic cell count and the isolation of bacterial agents involved in the process during lactation, as well as the sensitivity profile of these isolates to different antimicrobials. Thirty four ewes from the Santa Inês breed, raised under semi-intensive conditions and submitted to identical sanitary and nutritional management were evaluated before and after parturition, about 10 days before parturition, 15 days after parturition (dap), 30 dap, 60 dap and 90 dap (weaning). In the respective phases a clinical exam of the mammary gland was accomplished. The direct somatic cell count (SCC) and the California Mastitis Test (CMT) were carried out in the phases postpartum (15 dap, 30 dap, 60 dap and 90 dap) as well as the bacteriological analysis which was also accomplished in the experimental phase that preceded parturition when the ewes were not milked. Milk sampling was achieved by manual means. All ewes were submitted to lentivirus serology test. The variable data of SCC were tested for normality according to Kolmogorov-Smirnov and not attended the premise of normality were transformed into log base 10 (Log10). Therefore was performed the analysis of variance and contrast of means by Tukey test with significance level of P<0.05. A descriptive study of the variables studied was done by frequency distribution (%). The average somatic cell count in negative reactions to the CMT varied 387.896,08 cells/mL to 620.611,11 cells/mL and glands reagents ranged up to 6.730.514,50 cells/mL, but there was no influence in the different stages of lactation. The results allowed to conclude that subclinical mastitis represents a sanitary concern in the breeding of Santa Ines ewes. The phase that precedes parturition is particularly worrying and deserves more attention since there is a higher bacterial isolation percentage in apparently healthy glands. In association, it was also in the first 30 days of lactation (initial phase) that a high frequency of bacterial isolation was perceived, with coagulase-negative Staphylococcus as the agent isolated in higher percentage.
ISSN: 0100-736X
Appears in Collections:Artigos Científicos - FAMEVE/CCAST

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