Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/5697
Title: Detecção sorológica e molecular de Anaplasma marginale em búfalos na Ilha de Marajó, Pará
Other Titles: Serological and molecular detection of Anaplasma marginale in water buffaloes on Marajó Island, State of Pará, Brazil
metadata.dc.creator: SILVA, Jenevaldo Barbosa da
LOPES, Cinthia Távora de Albuquerque
SOUSA, Melina Garcia Saraiva de
GIBSON, André Felipe Bagarrão
VINHOTE, Wagner Marcelo de Souza
FONSECA, Adivaldo Henrique da
ARAÚJO, Flábio Ribeiro de
BARBOSA NETO, José Diomedes
Keywords: Búfalo
Anaplasma marginale
Anaplasmose
Sorologia veterinária
Estudos soroepidemiológicos
Ilha de Marajó - PA
Soure - PA
Issue Date: Jan-2014
Citation: SILVA, Jenevaldo Barbosa da et al. Detecção sorológica e molecular de Anaplasma marginale em búfalos na Ilha de Marajó, Pará. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 34, n. 1, p. 11-14, jan. 2014. Disponível em: <http://www.scielo.br/pdf/pvb/v34n1/02.pdf>. Acesso em: 26 jun. 2014. <http://dx.doi.org/10.1590/S0100-736X2014000100002>.
Abstract: The aim of the study was to test the molecular and serological prevalence of Anaplasma marginale in water buffaloes of the Marajó Island, State of Pará, Brazil. For serologic research were randomly selected 800 buffaloes and for molecular research 50 of these animals were randomly chosen. To quantify the serological prevalence we used the indirect enzyme linked immunosorbent assay (iELISA) with total antigen containing proteins outer surface. To quantify the prevalence molecular was used the polymerase chain reaction (PCR) involving gene amplification fragment larger surface protein 5 (MSP5). The prevalence of positive animals in iELISA was 25% (200/800). In the PCR we detected the presence of A. marginale in 2% (1/50) of animals. Although only one animal was positive in PCR, we found that it was negative in ELISA. The presence of the agent, even in low prevalence, shows that buffaloes can act as an important reservoir for transmission of the pathogen to cattle in northern Brazil.
URI: http://repositorio.ufpa.br/jspui/handle/2011/5697
ISSN: 0100-736X
Appears in Collections:Artigos Científicos - FAMEVE/CCAST

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