Please use this identifier to cite or link to this item: http://repositorio.ufpa.br:8080/jspui/handle/2011/6589
metadata.dc.type: Artigo de Periódico
Issue Date: 2014
metadata.dc.creator: RAMOS, Carlos Alberto do Nascimento
ARAÚJO, Flábio Ribeiro de
SANTOS, Rafaelle Cunha dos
MELO, Elaine Silva de Pádua
SOUSA, Letícia Carvalho de
VIDAL, Carlos Eugênio Soto
GUERRA, Neurisvan Ramos Guerra
RAMOS, Rafael Antonio do Nascimento
Title: Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
Citation: RAMOS, Carlos A.N. et al. Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 199-204, 2014. Disponível em: <http://www.scielo.br/pdf/bjm/v45n1/7118.pdf>. Acesso em: 24 abr. 2015. <http://dx.doi.org/10.1590/S1517-83822014005000039>.
Abstract: The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.
Keywords: Bovino
Anaplasma marginale
Testes de fixação do latex
MSP5
ISSN: 1517-8382
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - CCAST

Files in This Item:
File Description SizeFormat 
Artigo_DevelopmentAssessmentLatex.pdf659,08 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons