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metadata.dc.type: Artigo de Periódico
Issue Date: May-2014
metadata.dc.creator: ALMEIDA, Mauro Brito de
MALAQUIAS, Allan Costa
NASCIMENTO, José Luiz Martins do
OLIVEIRA, Karen Renata Matos
SILVA, Anderson Manoel Herculano Oliveira da
Title: Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
Citation: ALMEIDA, M. B. et al. Therapeutic concentration of morphine reduces oxidative stress in glioma cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 47, n. 5, p. 398-402, maio 2014. Disponível em: <>. Acesso em: 23 abr. 2015. <>.
Abstract: Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and levels of sulfhydryl groups (an indicator of the redox state of the cell). Morphine did not prevent the decrease in cell viability provoked by H2O2) but partially prevented lipid peroxidation caused by 0.0025% H2O2) (a concentration allowing more than 90% cell viability). Interestingly, this opioid did not alter the increased levels of sulfhydryl groups produced by exposure to 0.0025% H2O2), opening the possibility that alternative molecular mechanisms (a direct scavenging activity or the inhibition of NAPDH oxidase) may explain the protective effect registered in the lipid peroxidation assay. Our results demonstrate, for the first time, that morphine in usual analgesic doses may contribute to minimizing oxidative stress in cells of glial origin. This study supports the importance of employing concentrations similar to those used in clinical practice for a better approximation between experimental models and the clinical setting.
Keywords: Morfina
Estresse oxidativo
Peróxido de hidrogênio
Peroxidação de lipídeos
Analgésicos opioides
ISSN: 1414-431X
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

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