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Title: Estudo químico e atividade antibacteriana do fungo endofítico Scedosporium apiospermum de Bauhinia guianensis e de fungos da Serra de Carajás
Other Titles: Study chemical and activity antibacterina fungus endophytic Scedosporium apiospermum of bauhinia guianensis and fungi of Carajás Saw
metadata.dc.creator: CORDEIRO, Jorgeffson da Silva
metadata.dc.contributor.advisor1: MARINHO, Andrey Moacir do Rosario
Keywords: Química dos produtos naturais
Fungo endofítico
Fungo de solo
Agentes antiinfecciosos
Ensaio antimicrobiano
Scedosporium apiospermum
Bauhinia guianensis
Serra dos Carajás - PA
Pará - Estado
Issue Date: 22-Jun-2016
Publisher: Universidade Federal do Pará
Citation: CORDEIRO, Jorgeffson da Silva. Estudo químico e atividade antibacteriana do fungo endofítico Scedosporium apiospermum de Bauhinia guianensis e de fungos da Serra de Carajás. 2016. 206 f. Tese (Doutorado) - Universidade Federal do Pará, Instituto de Ciências Exatas e Naturais, Belém, 2016. Programa de Pós-Graduação em Química.
Abstract: Endophytic fungi are microorganisms that lives in association with the host species, and they're a promising source of important natural products. With a equal importance, the soil fungi also are capable of producing so many substances of economic value. Thus, the following work was objective to obtain bioactive composts produced by endophytic fungi M. acutistipula var. ferrea and soil Carajás Saw, besides the endophytic S. apiospermium from liana of Bauhinia guianensis Auble. The species M. acutistipula var. ferrea, were isolated 56 endophytic and 64 soil fungi. They were randomly, selected, 12 fungi to be reactivated in Petri dishes containing PDA medium. After reactivating the micro-extracts of 12 fungi were obtained as the adapted methodology Smedsgaar. The micro-extracts were evaluated by HPLC-DAD to determine the chromatographic profile and, also, submitted antimicrobial testing. The FSF 12 (trichoderma sp.), selected after the screening of chemical and biological profile was grown on a large scale in a solid medium (rice) and led to the isolation of five substances: fatty acid polyunsaturated (E1), 5'-inosil (E2), tyrosol (E3), harzialactone A (E4) and 2-anhydro mevalonic (E5). In relation to the fungus EJCP13 (S. apiospermium) where compounds isolated, triacyl (J1), ergosterol peroxide (J2), ergosterol (JA19), cerivisterol (JA29), methylester (JA24), dulcitol (D), and brefeldin A (JA). The compound Brefeldin A showed significant antimicrobial activity and, also, proved to be the major secondary metabolite in extracts from S. apiospermium. Thus, it decides to quantify a substance in the extract, which corroborate results, which indicate the presence of brefeldin A only in AcOEt and the hexane extracts. The isolation of the substances was performed by chromatographic techniques, such as CCVU and, monitoring, by ADCC. The chemical structures were elucidated with the aid of spectroscopic (1H and 13C NMR, HMQC, HMBC), and spectrometric (ESI-MS).
Appears in Collections:Teses em Química (Doutorado) - PPGQ/ICEN

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