Programa de Pós-Graduação em Neurociências e Comportamento - PPGNC/NTPC
URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/10669
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Navegando Programa de Pós-Graduação em Neurociências e Comportamento - PPGNC/NTPC por Orientadores "LIMA, Silene Maria Araújo de"
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Item Acesso aberto (Open Access) Análise comportamental e eletrofisiológica do uso de glicocorticoides no sistema nervoso central em modelos animais de depressão(Universidade Federal do Pará, 2017-01-31) CARDOSO, Keilla Gisele Mendonça; GOMES, Daniela Lopes; http://lattes.cnpq.br/0014255351015569; LIMA, Silene Maria Araújo de; CV: http://lattes.cnpq.br/8961057812067156Dexamethasone is a glucocorticoid widely prescribed in Medicine, used as immunosuppressant and anti-inflammatory as well as fluoxetine, which is an antidepressant, serotonin reuptake, which can be used at some point while aiming their respective purposes. The aim of this study was to evaluate the electroencephalographic abnormalities in animals administered with these drugs as well as the behavioral effects evaluated in an animal model of depression, forced swimming. The study was conducted in adult male Wistar rats subjected to administration of dexamethasone acute dose of 4 mg/kg, 24 hours before electrocorticographic record, and chronic, administered for seven days every 24 hours at a dose of 4 mg/kg i.p. Fluoxetine was administered at a dose of 5 mg/kg, for seven days orally with a similar tool the orogastric tube, gavage procedure. After administration, the electroencephalographic parameters of drug activity were recorded and analyzed. When comparing the acute administration of dexamethasone with chronic, there were no statistical differences, but there was a trend towards decreased Theta and Gamma force for chronic use. The group, who received fluoxetine averaged amplitude of 2,661 ± 0,5850 mV²/Hz x 10-3, proving fluoxetine efficacy in controlling depression caused by forced swimming. For the group received dexamethasone chronically and fluoxetine to reverse the power of the middle frame was 0.4758 ± 0.2514 mV²/Hz x 10-3, as there is no statistical difference between the dexamethasone group and dexamethasone fluoxetine, fluoxetine failed to reverse depressive symptoms caused by dexamethasone. In the forced swimming, the fluoxetine group had decreased fluctuation time, mean time of 45.33 ± 23.26 seconds, demonstrating that the group was not depressed. In the group administered with dexamethasone chronic form and assessed the possibility of reversing depressive disorder with fluoxetine immobility time average was 169.8 ± 24.5 seconds indicating that fluoxetine had no effect on the depression caused by chronic application of dexamethasone. We conclude from this study that the glucocorticoid cause changes in electrocorticogram and depression in the forced swimming test (FST). Fluoxetine got no effect on the rats subjected to the FST, after the use of dexamethasone.Item Acesso aberto (Open Access) Relação entre neurogênese hipocampal e história clínica de pacientes adultos portadores de epilepsia refratária do lobo temporal.(Universidade Federal do Pará, 2018-09-13) GAMA, Jessica Silva; SOUSA, Regina Célia Gomes de; http://lattes.cnpq.br/5576436464955236; LIMA, Silene Maria Araújo de; http://lattes.cnpq.br/8961057812067156Epilepsy is a neurological disorder characterized by the permanent predisposition of the brain to generate epileptic seizures, which affects about 1% of the world population. In Brazil, the disease can reach 2% to 4% of the population. Most patients have a good prognosis for drug treatment, however 30% of patients are refractory to treatment. Among the epilepsies that do not present a good prognosis is the Temporal Lobe Epilepsy (ELT). ELT is characterized morphologically by a selective loss of hippocampal neurons, concomitantly with increased hippocampal neurogenesis. It is believed that this exacerbated neurogenesis contributes to epileptogenesis (onset of epilepsy); however, the role of this increased neurogenesis in patients with TLE remains unknown. We used hippocampus from patients with epilepsy, who underwent a surgical procedure in which unilateral hippocampal resection was performed. From the analysis of the same, a possible relationship between neurogenic patterns and the evolution of the disease was investigated. Interviews with patients who underwent surgery demonstrated that the triggering event (precipitant) of epilepsy occurred in the first years of life of the patients. In addition, they were high intensity events, and also presented a high frequency of epileptic seizures and drug refractoriness. Immunohistochemical analysis of hippocampi showed equivalently that there was a perceptible dispersion of the granule cell layer, indicating a possible ectopic migration in labeling for neuroblasts (immature neurons), which are generated in neurogenesis. The results obtained in this work are pioneer, given that they are based on both clinical and histopathological findings, and the relationship between hippocampal neurogenesis and clinical history proposes a new research vision. These findings may also help in a new perspective of differential treatment in temporal lobe epilepsies.