Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF/ICS
URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/2312
O Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF) vinculado ao Instituto de Ciências da Saúde (ICS) da Universidade Federal do Pará (UFPA) apresenta um auto-impacto de inserção regional uma vez que se trata do único PPGCF na Região Norte pelo grande potencial de utilização da biodiversidade na região amazônica. Além de favorecer a fixação e atração de profissionais qualificados na área de Ciências Farmacêuticas na Região Amazônica.
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Item Acesso aberto (Open Access) Avaliação biológica dos extratos obtidos das sementes de Vatairea guianensis (Aublet).(Universidade Federal do Pará, 2011-03-28) SILVA, Cléia Tereza Lamarão da; SOUSA, Pergentino José da Cunha; http://lattes.cnpq.br/9909053957915090; CARVALHO, José Carlos Tavares; http://lattes.cnpq.br/4251174810000113Medicinal plants are widely used and culturally in the amazon to treat various diseases.Much of these plants has not been scientifically invesgated especially on the aspects related to biological activitie.The species selected in this work is Vatairea guianensis, used in traditional medicine to treat skin infections such as cutaneous mycoses. This study evaluated the antibacterial activity in vitro of hydroethanolic extract, hexane, chloroform and methanol Vatairea guianensis seeds by microdilution method for obtaining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Gram positive (Staphylococcus aureus and Enterecoccus faecalis) and Gram negative bacteria (Pseudomonas aeruginosa and Salmonella sp). The study also evaluated the healing activity on open wounds in rats by topical aplication of the hydroethanolic extract, represented by G1 positive control (fibrinase), G2 negative control (saline) and experimental groups( G3 dose 500mg/kg, G4 250mg/kg,G5 100mg/kg) for seven days and the acute oral toxicity. Histological analysis of the healing process was evaluated by means of conventional technique including color HE and Picrossirius for observation of the inflammatory reaction histomorphological characteristics and descriptive analysis of collagen deposition, respectively. All extracts showed antibacterial activity against all tested microorganisms with an MIC ranging from 3,12μg/mL to 50μg/mL and MBC values 6,25μg/mL to 100μg/mL. In histological analysis, the hidroethanolic extract decreased the intensity of inflammation in the groups G1 and G3, stimulates the synthesis of collagen type III em G1, G3 and G4 and increased collagen synthesis in G2 and G5. The experiment with the hydroethanolic extract obtained from seeds of Vatairea guianensis appeared to slow down the healing process effect by preventing the formation of hypertrophic scar, suggesting a modulatory effect by extract. The acute toxicity evaluation in mouse revealed that the hydroethanlic extract showed low toxicity by the oral proceedings.Item Acesso aberto (Open Access) Caracterização fitoquímica e avaliação da atividade anti-inflamatória e antinociceptiva do óleo fixo de Euterpe oleracea Mart.(Universidade Federal do Pará, 2009) FAVACHO, Hugo Alexandre Silva; SOUSA, Pergentino José da Cunha; http://lattes.cnpq.br/9909053957915090; CARVALHO, José Carlos Tavares; http://lattes.cnpq.br/4251174810000113Euterpe oleracea Mart. Is a typical palm tree from the Amazon, growing spontaneously in Pará and Amapá States, appreciated for its attractive beauty and for nutritional purposes. The fruit of Euterpe oleracea, commonly known as açaí, has been demonstrated to exhibit significantly high antioxidant capacity in vitro, and, therefore, may have possible health benefits. Phytochemical studies have revealed the presence of fatty acids, anthocyanins and steroids. The aim of this work was study the fatty oil of this species (OEO) on inflammatory and hyperalgesic models in vivo, in order to detect the possible involvement of its compounds on the inflammatory and algesic responses. The following experimental models were used: writing test in mouse, rat paw edema, granulomas testing rat, vascular permeability in rat, migration test in rats and ear erythema by croton oil in mice. The oral doses of 500, 1000 and 1500 mg/kg of OEO inhibited the number of writhes by 33,67%, 45,88% and 55,58%, respectively. The OEO produced dose-dependent effect, and the median effective dose (ED50) was 1226,8mg/kg, the OEO inhibited the inflammatory process by 29,18% on the 4 hour of the assay (edema peak) when compared to the untreated control. The daily administration of OEO for 6 days inhibited the granulomatous tissue formation in 36,66%. In the ear erythema by croton oil, the OEO, provoked the significant inhibitory effect of 37.9%. In the vascular permeability test, the OEO inhibited the vascular permeability in 54.5%. Pretreatment (60min) of rats with OEO significantly decreased neutrophil migration on carrageenan-induced peritonitis (with inhibition of 80.14%). The results suggest that OEO shows anti-inflammatory activity in both acute and chronic inflammatory processes, and analgesic activity, probably of peripheral origin.