Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF/ICS
URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/2312
O Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF) vinculado ao Instituto de Ciências da Saúde (ICS) da Universidade Federal do Pará (UFPA) apresenta um auto-impacto de inserção regional uma vez que se trata do único PPGCF na Região Norte pelo grande potencial de utilização da biodiversidade na região amazônica. Além de favorecer a fixação e atração de profissionais qualificados na área de Ciências Farmacêuticas na Região Amazônica.
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Item Acesso aberto (Open Access) Efeito de uma espécie do gênero Varronia sobre a viabilidade celular, atividade antimicrobiana, toxicidade dérmica aguda e o processo de cicatrização (in vitro e in vivo)(Universidade Federal do Pará, 2019-09-09) RIBERA, Paula Cardoso; FONTES JÚNIOR, Enéas de Andrade; http://lattes.cnpq.br/7056265073849866; https://orcid.org/ 0000-0002-6186-9581Tissue damage, particularly to the skin, results in damage to cell structures, layers, and lineages to the fullest extent. Under these conditions, wound healing is the physiological process responsible for tissue repair. Inflammation is an important stage in tissue repair and; therefore, a strong target for clinical studies. The species Varronia multispicata is popularly used for the treatment of bruises, with recently discovered anti-inflammatory and analgesic effects. The present work aimed to investigate the effect of aqueous extract of Varronia multispicata leaves (VAR01) on cell viability, antimicrobial analysis, dermal toxicity, and in vitro and in vivo healing. In the in vitro assays, there were evaluated the cell viability in BALB/c 3T3 murine fibroblasts, the antimicrobial action by the microdilution method for determination of minimum inhibitory concentration and petri dish culture technique for the minimum bactericidal concentration. Healing assays were performed in cultured fibroblast monolayers. For in vivo assays in the dermal toxicity test, female Wistar rats were used and divided into the following groups: saline, 100mg/ml, 200mg/ml, and 1000mg/ml of VAR01; as for healing evaluation, Mus musculus mice were used and divided into 4 groups: sham, negative control, treated (VAR01 10%), and positive control (Dersani®). V. multispicata kept the cells viable for 24h, with reduction of fibroblasts in the 48h period at a concentration of 500 µg/ml. It showed no antimicrobial activity, presented in vitro and in vivo injury contraction capacity, did not promote death or behavioral changes, did not cause changes in water and feed intake, weight gain, relative weight, and organ histological analysis, showed a reduction in alkaline phosphatase concentrations in the group treated with 100 mg/ml extract when compared as control group. It was also revealed a reduction in alanine aminotransferase levels in the 100 mg/ml extract-treated group when compared to the control group. However, a significant increase in TGP concentrations was found in the 200 mg/ml group when compared to the control group. While assessing the degree of irritation, VAR01 did not show an irritant profile when administered acutely topically. Therefore, the extract is safe and of low toxicity, promising in the process of tissue regeneration with possible modulation in the inflammatory pathway, being a stimulating result for the following steps of biological activity evaluation and elucidation of the healing process.Item Acesso aberto (Open Access) Investigação da toxicidade oral aguda e propriedades farmacológicas de uma espécie do gênero Cassytha(Universidade Federal do Pará, 2019-05-15) BARROS, Mayra Arouck; FONTES JÚNIOR, Enéas de Andrade; http://lattes.cnpq.br/7056265073849866; https://orcid.org/ 0000-0002-6186-9581Plants have been present in human culture since its inception, being used for several purposes, including for the treatment of diseases. This practice has been handed down from generation to generation. The evolution of knowledge, however, demands broader approaches on plant species with therapeutic potential, in order to ensure safety and validate its traditional use. Cassytha filiformis, a species of the genus Cassytha, is used in folk medicine to treat cancer, trypanosomiasis, kidney disease and gonorrhea. Among its proven activities, the antiplatelet, vascular relaxing, antioxidant, cytotoxic, antihypertensive, hepatoprotective, antiepileptic, diuretic and alpha-adrenergic receptor antagonist effects stand out. Among its secondary metabolites, alkaloids have been identified for therapeutic benefit. To date, however, there are no studies that support the safety of its therapeutic application or that explore possible anti-inflammatory properties as the basis for its therapeutic actions. Acute oral toxicity was assessed according to OECD 425. Starting from an administration of 2000 mg / kg (v.o) of extract in rats, the hippocratic signs of toxicity, spontaneous locomotor activity, weight gain, water and food consumption, as well as relative body weight and hematological patterns at the end of the period. The antinociceptive activity was evaluated in mice, using the acetic acid induced writhing test and the formalin test (CEUA nº 1050140817). The cutoff dose of the extract did not promote hipocratic signs of toxicity or death. There were also no changes in feed and water consumption or weight gain patterns. The evaluation of the relative weight of organs (liver, kidneys, stomach and heart) and hemogram showed equivalent standards between treated and control animals. Cas01 also did not impair the locomotor activity of the animals. Cas01 was shown to have no influence on the contortions induced by acetic acid, nor did it promote significant alterations on formalin-induced biphasic nociception. These findings demonstrate for the first time that Cas 01 is a xenobiotic of low acute oral toxicity. They also demonstrate that their therapeutic actions do not involve nociceptive or inflammatory mechanisms.