Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF/ICS

URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/2312

O Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF) vinculado ao Instituto de Ciências da Saúde (ICS) da Universidade Federal do Pará (UFPA) apresenta um auto-impacto de inserção regional uma vez que se trata do único PPGCF na Região Norte pelo grande potencial de utilização da biodiversidade na região amazônica. Além de favorecer a fixação e atração de profissionais qualificados na área de Ciências Farmacêuticas na Região Amazônica.

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    Atividade antitumoral em células de câncer gástrico e atividade antioxidante de extratos de Eugenia patrisii vahl
    (Universidade Federal do Pará, 2020-09-09) REIS, Herald Souza dos; AMARANTE, Cristine Bastos do; http://lattes.cnpq.br/4101983776191966; https://orcid.org/ 0000-0002-8602-8180; PRADO, Alejandro Ferraz do; http://lattes.cnpq.br/7016475842644161; https://orcid.org/ 0000-0001-7495-9837
    Several antineoplastic drugs are natural origin or from derived compounds and plants are one of the primary sources of substances for this sort of drugs. Studies with Myrtaceae family plants, demonstrated several effects such as anticancer and antioxidant. A plant in this family that may have biological activities is Eugenia patrisii Vahl, popularly known as Ubaia-rubi-da-amazônia, a plant native to the Amazon Region. Therefore, the work objective was to evaluate the antitumor and antioxidant activity of hydroalcoholic extracts of the leaf and stem of the E. patrisii species. The chemical profile of the extracts was done using thin layer chromatography. The antioxidant activity was assessed by the DPPH test. For antitumor activity, a cell viability test was performed using the MTT method on gastric cancer lines (ACP02, ACP03 and AGP01) and normal lines (VERO and MN01). Gel zymography was also performed to assess the activity of MMP-2 and MMP-9. The data obtained were analyzed using the Graph Pad Prism program version 8.0. The tests used were variance analysis (ANOVA) and Test t Student, with p <0.05 being considered significant. The plates derived from thin layer chromatography showed presence of terpenes, flavonoids, phenolic compounds and absence of coumarins and alkaloids. The extracts were shown to have antioxidant capacity through the DPPH assay. In the cell viability test, the extracts showed a high potency against gastric tumor lines and did not affect the normal line. In addition, the leaf extract decreased MMP-2 and MMP-9 activity in gel. The extracts have terpenes, flavonoids and phenolic compounds that have an antioxidant activity of 82% compared to the standard and antitumor against gastric lines ACP03 and AGP01 with the potential to isolate compounds of pharmacological interest.
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    Efeitos do antioxidante tempol nas alterações bioquímicas e estruturais induzidas pela metaloproteinase de matriz 2 no coração
    (Universidade Federal do Pará, 2020-09-11) GONÇALVES, Pricila Rodrigues; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390; https://orcid.org/ 0000-0002-3328-5650; PRADO, Alejandro Ferraz do; http://lattes.cnpq.br/7016475842644161; https://orcid.org/ 0000-0001-7495-9837
    MMP-2 expression is elevated in many cardiovascular pathologies such as myocardial infarction and heart failure, where tissue remodeling and inflammatory responses are disturbed. Changes in ECM homeostasis can lead to cardiac dysfunction. The most analyzed MMPs in relation to cardiac dysfunction are MMP 2 and MMP-9. ROS inhibitors, as antioxidants, have been shown to reduce the expression of MMP-2 in vascular tissue. Thus, antioxidants with Tempol have great potential to act on these mechanisms. Therefore, we evaluated the activity and purity of rhMMP-2 using the zymogram and SDS-PAGE silver-stained method. The animals were divided into 4 treatment groups. Group 1: received 0.9% saline; group 2: Tempol 18 mg / kg / v.o; group 3: MMP-2 150 ng / kg / i.p; group 4: Tempol 18 mg / kg / v.o + MMP-2 150 ng / kg / i.p; for 4 weeks. At the end of the treatment, the heart was collected for quantification of collagen, quantification of ROS by fluorescence microscopy and immunofluorescence for TNFα and TGF-β. After the analysis, our results showed that rhMMP-2 was pure and active and that there was no difference in the average weight of the animals (P> 0.05). In the group treated withrhMMP-2 and Tempol, there was a decrease in the heart weight / body weight ratio, compared to the control group (P <0.05). Tempol was able to decrease collagen in the heart of animals treated with rhMMP 2. We also saw rhMMP-2 increased ROS in the heart, which was prevented by Tempol. RhMMP-2 also led to an increase in TNF-α and TGF-β in the heart, however TGF-β was reduced by Tempol. In conclusion, rhMMP-2 infusion increased cardiac ROS, which can lead to oxidative stress, with a consequent increase in TNF-α and TGF-β, which can result in a heart with a pro-fibrotic and inflammatory profile. However, Tempol was able to reduce interstitial collagen, inhibit the increase in ROS, TNF-α, TGF-β and increase catalase in the heart. Having Tempol, the potential to inhibit factors that lead to oxidative stress, inflammation and cardiac fibrosis.
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