Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF/ICS

URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/2312

O Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF) vinculado ao Instituto de Ciências da Saúde (ICS) da Universidade Federal do Pará (UFPA) apresenta um auto-impacto de inserção regional uma vez que se trata do único PPGCF na Região Norte pelo grande potencial de utilização da biodiversidade na região amazônica. Além de favorecer a fixação e atração de profissionais qualificados na área de Ciências Farmacêuticas na Região Amazônica.

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Caracterização farmacognóstica, química, físico-química e estudos preliminares de pré-formulação da Arrabidaea chica (Humb. & Bonpl.) B. Verlt.
(Universidade Federal do Pará, 2008-10-22) ALVES, Mauro Sérgio Marques; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
This work aimed the physical, chemical, physico-chemical and preformulations characterizing of Arrabidaea chica (Humb. & Bonpl.) B. Verlt., belonging to the family of Bignoniacae, which receives several denominations, and in the Amazon region is known popularly as pariri, that is widely used in popular medicine for the treatment of many diseases, such as skin infirmities caused by dermatitis and eczema. The use of natural products from vegetal origin requires the pharmaco-botanical quality control and pureness analysis which sets the species technical specifications. Therefore, it was made an anatomical description of the young and mature leaves of the plant from observations made at the optic microscope in histological cuts. The pharmacological tests included the determination of the granulometrical distribution of the plants powder, determination of the humidity purport and of total ashes and, about the dyeing, it was made pH, relative density and solids purport determinations, and also it was made the chemical prospection, the chromatographic profile by thin-layer chromatography (TLC) and the highperformance liquid chromatography (HPLC), besides the valuation of its microbial activity. To the powder as well as the dyeing of the A. chica observed the profiles by spectroscopy in the infrared region and thermal profiles by TGA and DTA. Some studies of preformulation were made by spectroscopy in the infrared region and thermal analysis (TGA and DTA) of the binary mixtures of the formula adjuvants suggested to spread the dye, with the purpose to evaluate possible incompatibilities between the dye and these elements. The results obtained evidenced that the A. chica dye demonstrated to adjust to the aim demanded, moreover it assured the compatibility to the tested adjuvants to constitute the proposed formula, once none clue of physical or chemical incompatibilities were observed among them. The studies provided relevant basis to the development of the proposed formula, seeking to obtain quick results with the wished accuracy.
Acesso aberto (Open Access)
Caracterização química e físico-química e estudos preliminares de planejamento da formulação fitoterápica semi-sólida contendo tintura de Calendula officinalis L.
(Universidade Federal do Pará, 2008-12-29) NUNES, Kariane Mendes; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
The phytotherapy is a form of medicinal therapy that has been grown noticeably over the years, however, despite the broad use of phytomedicines, the quality of these drugs is often poor and questionable. Among the several medicinal plants used as herbal medicine, the Calendula officinalis L. is known for its anti-inflammatory, antiseptic and healing effects. Thus, this present study aimed to enhance and consolidate the use of methods of pharmaceutical technology in the development of phytotherapeutic agents, through the completion of preliminary studies of the planning of a semi-solid formulation phytotherapic containing tincture of Calendula officinalis L., for the quality control of the stages of their development. In the physical characterization and physical chemistry of the dust and tincture of calendula were obtained farmacognóstics specifications consistent with the literature, and to establish the identity of the plant material through the detection of the chemical marker rutin by Thin-Layer Chromatography (TLC). Through the validation of the method, that presented parameters recommended by existing legislation, was given 463 μg/mL of rutin in tincture. The infrared (IR) spectra showed characteristics of rutin in the lyophilised extract, in addition to demonstrate the permanence of these bands after mixing with the excipients of the formulation. The thermoanalytic techniques confirmed the compatibility between the excipients and lyophilisate of calendula extract. In the preliminary assessment of the stability of the gel formulation remained stable during the cycles in greenhouse (45 ± 2 0C) and temperature (25 ± 2 0C). In the preliminary study of the gel, permeation tended to favor the permeation of total flavonoids as rutin for receiving phase. These results demonstrate the importance of employment of protocols for quality control of raw materials plant, besides the establishment of technological methods for the production of phytotherapic.
Acesso aberto (Open Access)
Desenvolvimento e caracterização tecnológica de micropartículas de arrabidaea chica (h & B) B. Verl. obtidas por spray dryer
(Universidade Federal do Pará, 2012-08) SAMPAIO, Rita de Cássia Almeida; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
This work aimed the technological development of particulate dosage forms obtained from extraction solution (tincture) from aerial parts of Arrabidea chica (Pariri), using the spray drying. We investigated the influence of processing aids (maltodextrin and arabic gum, alone and in a mixer) in drying performance and product properties. A. chica tincture was concentrated on a rotary evaporator to obtain the concentrated extract (CE) which was added to the processing aids and subjected to spray drying. The drying process was assessed through determination of product recovery and thermal efficiency. Microparticles (ES) were evaluated for residual moisture, water activity, average particle diameter, bulk density, packing density and flow properties, thermal profile and IR spectroscopy. The chemical monitoring was performed by UV-vis spectrophotometer, using chemical marker flavonoids (Ft), polyphenols (Pt) and total tannins (Tt) in the A. chica. The antioxidant activity of the product was evaluated by DPPH and chemiluminescence. The moisture content of the ES had their values in the range 2.77 to 4.69% being within the specified. The water activity values were below 0.2, which supports the physical and chemical stability and microbiological of dried products. The percentage of degradation of chemical markers in ES regard to CE was in the range 24-46% for Ft, 48-56% for Pt and 53-72% to Tt, which may be associated with thermal degradation and oxidation of the same compounds. In TLC analysis, tincture, CE and ES showing the luteolin and kaempferol patterns indicate that the drying process does not cause the loss of these compounds. The particle size distribution shows that the ES showed an average diameter of about 10 μm and the particles had a spherical morphology and with some rough surface. The flowability indices and accommodation of the powders obtained results are typical of powders with low flowability and compressibility characteristics. The antioxidant activity of ES presented values between 32.17 to 44.53 mg / mL. In the colorimetric analysis, the parameters proved the yellowish-red color. The recovery of product was in the range 60-65% and the thermal efficiency values were between 36% and 39%. Analyses of infrared spectroscopy and thermal analysis proved important tools in the physico-chemical characterization and quality control systems microparticulate obtained.
Acesso aberto (Open Access)
Estudos de pré-formulação e formulação de Heliotropium indicum (L.) DC (Boraginaceae)
(Universidade Federal do Pará, 2010-01-14) COSTA, Russany Silva da; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
Heliotropium indicum L. (Boraginaceae) is a shrub, reaches up to 70 cm high. It is known as “fedegoso” in the north and northeast of Brazil. Because of the biological potential of this species, this work aims to determine the quality parameters of the vegetable drug to the final product and evaluate the antimicrobial activity of the leaves of H. indicum L. The purpose of obtaining a phytotherapic formulation semisolid, we used the parameters of quality control physical, chemical and physicalchemical described in the Brazilian Pharmacopoeia and others literature. The vegetable drug was classified as coarse powder, showed values of loss on drying and total ash of 12.88% and 17.14%, respectively. The thermogravimetric analysis of the powder and the extract showed that both had good physical stability up to 180 °C. The spectra in the region IR showed an increase in the intensity of the absorption bands of the extract, which may relates to the extraction of chemical constituents of the cellular matrix. The prospecting chemical extract confirmed the presence of classes of secondary metabolites already reported in literature. The chloroform fraction suggests the presence of alkaloids by precipitation test with Dragendorff reagent. The TLC and HPLC showed a possible presence of the same substance in the alkaloidal and hexane fractions. The crude extract inhibited the growth of Staphylococcus aureus with halos of 12.5 mm ± 0.707 and 10.5 ± 0.707 mm for concentrations of 500 and 250 mg / mL, respectively. The physical mixtures of the extract with adjuvant pharmaceutical used to develop the phytotherapic formulation showed no physical incompatibility and no significant changes in the absorption profile of the compounds analyzed. The phytotherapic formulation semi-solid has a good stability after the preparation, after the submission of the gel by centripetal force and after the action of heat stress.
Acesso aberto (Open Access)
Estudos de pré-formulação e formulação de Vismia guianensis (Aubl.) Choisy
(Universidade Federal do Pará, 2010-01-15) CAMELO, Sarah Regina Pereira; VASCONCELOS, Flávio; http://lattes.cnpq.br/3695753129639448; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
The obtention and evaluation of a new herbal formulation from Vismia guianensis (Aubl.) Choisy was determined by parameters of quality control. Physical-chemical characterization in plant raw material dried and crushed, its extract of the tincture and semi-solid formulation with antimicrobial activity. For the characterizations specific parameters for herbal drugs contained in the Brazilian Pharmacopoeia 4th Ed were used, the thermal analysis: thermogravimetry, differential thermal analysis and differential scanning calorimetry and infrared and ultraviolet spectroscopy. The evaluation of the antimicrobial activity by the disk diffusion method in solid identified the sensitivity of S. aureus (ATCC 25923) to the dried extract dissolved in DMSO at concentrations of 500, 250, 125 and 62.5 mg / mL. The HPLC drawing the profile of the composition of the sub-fractions A and B from the ethyl acetate fraction of the tincture and showed the range of maximum absorption at 290 nm similar to the maximum capacity of emodin being used a reference standard and an external marker. The method validation was performed by ultraviolet spectrophotometry, demonstrating a selective method, linear, repeatable and robust. Thermal analysis showed possible incompatibilities between the binary mixture of the extract of the tinsture with hydroxyethylcellulose and propylene, then obtaining non-homogeneous gel due to precipitation of proteins, triggered by the polyphenol-protein interaction. A preliminary evaluation of the stability of the formulation obtained remained within the normal parameters, show results within acceptable limits for the centrifuge tests, thermal stress and organoleptic characteristics.
Acesso aberto (Open Access)
Obtenção e caracterização de carreadores lipídicos nanoestruturados a partir de gordura vegetal de murumuru (Astrocaryum murumuru Mart.)
(Universidade Federal do Pará, 2016-05-16) SENA, Luann Wendel Pereira de; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
The nanostructured lipid carrier (NLC) have been proposed as a carrier’s category for pharmaceutical and cosmetic ingredients and an increasing of interest due to a series of advantages when compared to conventional formulations. The Amazonian vegetable fats are considered lipids arrays of great potential for the production of NLC from topical administration, according to the low toxicity and biocompatibility, beyond their emollient properties, protector and moisturizer in the skin. The murumuru (Astrocaryum murumuru Mart.) is an amazon palm and the utility of its fat is promising, because it adds value to the products, favour the growth in the region, in addition to using these resources in a sustainable way. The objective of this research was to obtain and characterize the NLC from the vegetable fat of Murumuru (Astrocaryum murumuru Mart.) by the homogenization technique under high pressure to hot, using ketoconazole as a drug modelling. The gas chromatography showed lauric acid (48,1 %), myristic acid (26,6 %) and oleic acid (8,4 %), as main constituents. The physical-chemical characterization showed acid, iodine, saponification, peroxide, refraction and density values within the limits and standards recommended. The isolated raw materials and the NLC obtained were evaluated using differential scanning calorimetry, where the results about the isolated raw materials used support as was described in Literature and the NLC demonstrated a crystalline structure less ordained. In the tested formulations, the nanoparticles showed average size between 98,60 and 161,56 nm, polydispersibility index between 0,115 and 0,276, zeta potential higher than -30mV and encapsulation efficiency near 100 %. The Transmission Electron Microscopy indicated spherical aspect of the nanoparticles and the! clearance profile showed a zero order kinetic model, with the release of 70,9 % of the encapsulated drug in 8 hours. The nanoparticles remained stable during a period of 60 days. This research showed that the murumuru fat obtained an effective system, with small sizes particles and penetrability in the skin, making possible the formulation propagation in pharmaceutical and cosmetic uses.
Acesso aberto (Open Access)
Obtenção e caracterização de formulação fitoterápica contendo extrato e tintura padronizados de arnica montana l e aesculus hippocastanum l
(Universidade Federal do Pará, 2015-12-18) ARNILLAS, Elyan Andrade Pueyo; VIEIRA, José Luiz; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
Because of the wide dissemination and use of medicinal plants, industries are producing products based on plant species, such as extracts and tinctures, which are mainly used by pharmacy with handling. However, it is questionable collateral for the vast majority of these products as to their efficacy, safety and quality, and may bring risks to the user's health. So, it is important to perform quality control for herbal products, according to current legislation. The formulation that gave rise to the research is a carbopol gel containing Arnica montana L dye and Aesculus hippocastanum L extract. The present study aimed at obtaining and characterization of said formulation and validate the use of methods of pharmaceutical technology by performing the standardization of Aesculus hippocastanum L extract and Arnica montana L dye, to the physio-chemical evaluation were performed: microbiological control, organo-leptical characterization, determination of pH, density, dry, loss on drying, determine the level solids, rheological behavior, concentration, lyophilization and chemical prospecting of plant inputs. For the phytochemical analysis was performed Thin Layer Chromatography of Arnica tincture (TLC) and Thin Layer Chromatography In Comparative (TLCC) Aesculus extract, Infrared and Thermal Analysis of both extracts as the formulation. For physicochemical characterization and validation of analysis Reports of the two plant species suppliers the results are within the parameters established by the Brazilian Pharmacopoeia Fifth Ed. The microbiologic control was to prove the absence of pathogenic microorganisms, the results indicated microorganisms absence of, for microbial contamination of the inputs can change the stability of the final product and further characterize risk of infection to the user. The chemical prospecting of plant species, was to identify relevant secondary metabolites, in the case of A. hippocastanum indicated the presence of saponins, represented by escin which is responsible for the pharmacological activity, was an important clue to the certification of the authenticity of the raw material in study. The viscosity of vegetable raw materials and formulation were determined by the rheometer of Brookfield, the extracts showed fluid behavior and Newtonian formulation showed a pseudo plastic behavior. The result of the Thin Layer Chromatography (TLC), assisted in the identification of the sample and possible tampering that could have occurred as well as the TLCC served to demonstrate the selectivity of the method as identified the presence of aescin, substance responsible for pharmacological activity Chestnut from India. In the validation of Arnica montana L. dye, it was detected by HPLC the presence of the rutin in the sample in nature with retention time 10.84 min. The infrared analyzes the plant species were observerd bands that may indicate the presence of the major chemical markers in the case Arnica indicated the presence of the rutin and escin in the presence of horse chestnut extract. In the thermal analysis TG / DTA, we can say that the first decomposition event occurred for the samples to 150 ° C, corresponding to weight loss, the values were 6.5 and 8% for the chestnut and arnica respectively. All results were significant for the characterization and quality control, used as benchmark for the standardization of the extract and tincture, the information obtained was also used for the certification of suppliers of extracts and tinctures for drugstores in Belém.
Acesso aberto (Open Access)
Obtenção e caracterização de nanoemulsão óleo em água a partir de óleo de açaí (Euterpe oleracea M.)
(Universidade Federal do Pará, 2015-05-13) CONTENTE, Denise Maria Loureiro; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
Nanoemulsions have been proposed as an option for drug delivery current systems and increasing interest due to several advantages when compared with traditional formulations. Vegetable oils are considered pharmaceutical ingredients of great value as lipid matrix of these systems, such as oil açaí, of Amazonian origin oil, which has a number of medicinal benefits such as antioxidant and moisturizing activity, and biocompatibility, increases skin elasticity and acts as a physical barrier. The objective of this study was to obtain nanoemulsion O / W, from açaí oil, surfactant and water, through the phase inversion temperature method for topical use. Açaí oil was characterized by physical-chemical tests and on the profile of fatty acids by gas chromatography (GC), spectrometry in the infrared (FT-IR) and thermal analysis by thermogravimetry (TG) and differential scanning calorimetry (DSC). The surfactant, ketoconazole and their binary mixtures with oil açaí were evaluated by DSC. Obtained nanoemulsions making use of açaí oil, BrijTM CS20, water and ketoconazole. The characterization of nanoemulsions was performed on the droplet size, polydispersity index (PI), the zeta potential (PZ), morphology and encapsulation efficiency (EE). The açaí oil showed acid value (3.78 mg KOH / g), iodine value (71 gl2 / 100g), saponification number (199 mg KOH / g), refractive index (1.470) and density (0,950g / ml). In GC analysis showed 68.05% of unsaturated fatty acids, being 47.58% oleic acid. Oxidative stability of açaí oil Rancimat was about 11.79 hours. The FT-IR spectrometric analysis confirmed bands suggestive of unsaturated fatty acids and thermal analysis it was observed that the thermal degradation occurs above 200 ° C. The combinations of raw materials analyzed by DSC was observed that the incorporation of ketoconazole with açaí oil. The nanoemulsion B10 with 0.5% ketoconazole showed 98.31% EE. The transmission electron microscopy showed droplets with spherical shape and after study of PZ (-25.53 ± 10.04 mV), PI (0.37 ± 0.04) and size (128.53 nm ± 10.04) by 30 days, nanoemulsion showed little variation. Thus, it can be said that açaí oil has the potential to be used as a pharmaceutical ingredient can be used as oil phase nanoemulsions loaders of fat-soluble substances.
Acesso aberto (Open Access)
Obtenção e caracterização de sistemas líquido cristalinos contendo óleo de açaí (euterpe Oleraceae Mart.)
(Universidade Federal do Pará, 2015-03-20) PEREIRA, Rayanne Rocha; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
The Euterpe oleracea Mart. is an Amazonian palm tree, popularly known as acai. From the pulp of the acai fruit can extract the fixed oil, consisting mainly of fatty mono and polyunsaturated acids. This oil is a promising raw material for the pharmaceutical industry, which can be exploited by their biological activities and as a raw material in the development of sustained drug delivery systems. Liquid crystals are examples of sustained drug delivery systems. The objective of this study was to develop liquid crystalline systems containing acai oil as the oil phase and the Procetyl AWS as surfactant. Initially acai oil was analyzed for its physical and chemical characteristics. Gas chromatography revealed oleic acid (47.58%), palmitic acid (24.06%) and linoleic acid (13.58%) as major constituents. The physicochemical characterization showed values of acidity, iodine, saponification, refraction and density within the limits and recommended standards. Spectroscopy in the infrared region showed characteristic bands of acai oil. Thermogravimetry açai oil showed a unique event that possibly due to the degradation of fatty acids, this event is located in the temperature range of 241.71 to 481,14ºC. The acai oil induction time was 11,79h. The pre-formulation studies with acai oil and the surfactants showed no evidence of chemical interaction or resulting degradation of binary mixture between the oil and the Procetyl AWS. Formulations were prepared 36 with the aid of ternary diagram between these formulations were identified hexagonal and cubic mesophases. The rheological analysis showed pseudoplastic behavior (n <1) with plastic characteristics that refer to the Bingham fluids. The stability study conducted over 30 days did not reveal any signs of instability of the evaluated systems. The realization of this study demonstrated that the acai oil is a promising raw material in the development of sustained drug delivery system.
Acesso aberto (Open Access)
Obtenção, caracterização e microencapsulação de extratos ricos em antioxidantes naturais a partir do co-produto das amêndoas do tucumã (astrocaryum vulgare mart.)
(Universidade Federal do Pará, 2019-07-01) FERREIRA, Lindalva Maria de Meneses Costa; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994; https://orcid.org/ 0000-0003-1691-1039
The purpose of this work was to microencapsulate the extract of the co-product of tucumã seeds (Astrocaryum vulgare Mart.) by spray drying using maltodextrin as a encapsulating agent. The techniques of thermogravimetry and Infrared analysis, water activity, scanning electron microscopy and electrophoretic mobility served to characterize the microencapsulated extract. The total of polyphenols, flavonoids and condensed tannins of the microencapsulated extract was determined by UV spectroscopy and identified by ultra high performance liquid chromatography, the antioxidant activity was measured by ABTS and DPPH. The microparticles showed heterogeneity, spherical structure and no cracks, confirming the efficiency of the microencapsulation process using maltodextrin as an encapsulating agent. The microencapsulated extract values of phenolic compounds content entre 130 mg ± 0,024 e 104,7 mg EAG/100g ± 0,024, Total flavonoids 27,17 ± 0,002 e 25,36 mg QE/100g ± 0,004) and condensate tannin 62,07 100g ± 0,137 e 47,95 mg CE/100g ± 0,087 and good antioxidant activity for the two methods tested. The ultra high performance liquid chromatography analysis identified the presence of gallic acid and caffeic acid. In general, the results showed good utility of the tucumã co-product in the form of microparticles rich in antioxidant compounds, which can be exploited for future application in the food area as a functional food.
Acesso aberto (Open Access)
Simulação computacional de carreador lipídico nanoestruturado contendo gordura de cupuaçu (Theobroma Grandiflorum)
(Universidade Federal do Pará, 2017-10-19) VIEIRA, Ana Paula Bastos Ferreira; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994
The constant evolution of the drug delivery systems boosted the search for new strategies, to obtain potentially promising formulations. Computational simulation and molecular modeling can provide effective contributions to the design and evaluation of complex systems, such as lipid nanocarriers containing natural inputs. In this work, a nanostructured lipid carrier (NLC) was proposed using triacylglycerols of cupuaçu fat and capric/caprylic acid, carnauba wax, tween 80®, pluronic® and an enantiomer of ketoconazole. Experimental tests were carried out to evaluate the crystallinity and thermal events of the lipid mixtures, which demonstrated that the M03 mixture had a lower enthalpy value (-15,17 J/g) and greater widening of the diffractogram peaks, suggesting a lower crystallinity structure. Computational simulations were performed to elucidate 3D structures, to study molecular and electrostatic properties, as well as intermolecular interactions with the drug. The molecular docking of M03 with ketoconazole showed interaction of the hydrogen bond type, with bond distance of 2,62 to 3,50Å and affinity energy of -5,3 Kcal/mol. The three-dimensional CLN model showed good molecular distribution and after simulation of molecular dynamics, the system remained stable (energy -1895,47 KJ / mol), with moderate to weak hydrogen bonding interactions and a distance of 2,45 to 3,50Å. RDF profiles established demonstrate strong interactions between ketoconazole and tween 80, between water and pluronic, between pluronic and SOO, and between tween 80 and SOO, all interactions occurring within the radius ≥2Å of distance. These results can be used as support to guide the future development of a CLN formulation, anticipating the experimental tests and helping to obtain thriving formulations.

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