Dissertações em Ciência Animal (Mestrado) - PPGCAN/Castanhal
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/2337
O Mestrado em Ciência Animal teve início em 1999 junto à CAPES/MEC e funciona no Programa de Pós-Graduação em Ciência Animal (PPGCAN) do Campus Universitário de Castanhal (CCAST) da Universidade Federal do Pará (UFPA), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) e Universidade Federal Rural da Amazônia (UFRA).
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Navegando Dissertações em Ciência Animal (Mestrado) - PPGCAN/Castanhal por Orientadores "BARBOSA NETO, José Diomedes"
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Item Acesso aberto (Open Access) Aspectos clínico-patológicos do envenenamento experimental por Caudisona durissa em equinos(Universidade Federal do Pará, 2012-09-28) LOPES, Cinthia Távora de Albuquerque; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557The clinic-pathological picture and laboratory findings in horses experimentally inoculated with the venom of Caudisona durissa terrificus (Crotalus durissus terrificus, according to the former nomenclature) are described. The purpose of this study was to contribute to the understanding of this type of snake accident in horses. The lyophilized venom was diluted into 1ml of a 0.9% saline solution and was inoculated subcutaneously into five horses, at the doses of 0.12mg/kg (one horse), 0.066mg/kg (two horses) and 0.03mg/kg (two horses). The venom caused death of the horse that had received 0.12mg/kg, and of one horse of the two that had received the dose of 0.066mg/kg. The clinical course varied from 27h27min to 52h29min. The second horse inoculated with 0.066mg/kg recovered within 12 days after inoculation. The dose of 0.03mg/kg had a course of 6 to 10 days but did not cause fatal envenomation. The clinical picture in the horses was characterized by swelling of the inoculation site and adjacent areas, by apathy and lowered head, locomotory alterations shown by dragging of the hoves on the ground, decubitus and difficulty to get up, reduction of auricular, palatal, upperlip and threat reflexes, and increase of heart and breathing frequency. The laboratory examination revealed leukocytosis and lymphocytosis in two horses. There was increase of the creatine-kinase (CK), lactic dehydrogenase (DHL) and ureia; reduction in the seric levels of calcium, phosphorus and magnesium. The time of activated partial tromboplastina (TTPA) increased in the horses that died. Post mortem findings were edema of the subcutaneous tissue at the inoculated shoulder, suffusions in the epicard of left and right heart ventricles, bladder replete with urine containing a white-yellowish sediment, and hemorrhagic areas in its mucosa. Histopatologic examination revealed the liver parenchyma with diffuse moderate vacuolation affecting predominantly the intermediate area of the hepatic lobe, and slight dilation of the sinusoides in some areas; in the kidney slight dilation of the uriniferous tubules mainly in the cortex.Item Acesso aberto (Open Access) Determinação dos níveis de fósforo, cobre, cobalto e zinco em bubalinos criados nos municípios de Soure, Salvaterra, Breves e Cachoeira do Arari, na ilha de Marajó, estado do Pará(Universidade Federal do Pará, 2010-05-31) PINHEIRO, Cleyton Prado; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557We studied 104 buffaloes, adults, without distinction of race and sex, reared extensively, without supplementation, in native pastures of low nutritional quality, in the municipalities of Breves, Cachoeira do Arari, Salvaterra and Soure, Marajo Island, Pará. Held collection of liver, bone and blood of 26 animals in the municipality of Salvaterra, 38 animals in the municipality of Soure, 20 animals in the municipality of Breves and 20 animals in the municipality of Cachoeira do Arari. Determination was performed to determine the levels of phosphorus in the blood serum and bone ash and specific gravity on bone and cobalt, copper and zinc in liver tissue. It was observed that the average phosphorus concentrations in blood serum (6.26 mg/dl) and bone (10.77 %), the percentage of ash (60.87 %) and specific gravity (1.59 g/ml) of bone were lower than the critical levels established for cattle, characterizing phosphorus deficiency. The average concentrations of copper (5.57 ppm) and zinc (27.05 ppm) was considered low when compared with reference values, characterizing disability for these elements. In the case of cobalt when considering the values detectable and undetectable by the methodology it was observed that 51.92% of the animals had levels below the reference, indicating the occurrence of cobalt deficiency in these animals. It is emphasized that the deficiencies of copper and zinc showed a more severe since all animals studied showed low levels of these elements.Item Acesso aberto (Open Access) Encefalomielite equina Leste na Ilha de Marajó, Pará(Universidade Federal do Pará, 2012-02-29) CAMPOS, Karinny Ferreira; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557Nine cases of equine encephalomyelitis were studied in the Marajó Island, State of Pará, Brazil. The animals had difficulty in maintaining a station, walk in a circle, marked depression, eyelids closed, tongue paralysis, muscle tremors, bruxism, anorexia and dehydration. Some had their ear and eyelid reflexes diminished, decreased tongue tone and tachycardia. Position of self-hearing was observed frequently. The animals were often found leaning on tree trunks and fences to keep themselves on station. At necropsy, they showed hemorrhage of the meninges and spinal cord, and some animals also showed adhesion of the meninges. Histologically there was diffuse encephalitis affecting mainly the gray matter, with meningitis and choroiditis. It was observed the presence of perivascular cuffs consisting of mononuclear inflammatory cells. In two animals it was possible to identificate the Eastern equine encephalitis virus by semi-nested reverse transcription polymerase chain reaction (semi-nested RT-PCR).Item Acesso aberto (Open Access) Estudo das deficiências minerais em vacas em lactação da bacia leiteira do município de Rondon do Pará, estado do Pará(Universidade Federal do Pará, 2014-12-10) BOMJARDIM, Henrique dos Anjos; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557We conducted a study of mineral deficiencies in lactating cows from dairy herds belonging to 13 properties of the milk basin of Rondon do Pará, in the state of Pará. We determined the levels of phosphorus (P), specific gravity and percentage of ash in the bone and levels of copper (Cu), cobalt (Co), selenium (Se), zinc (Zn), iron (Fe) and manganese (Mn) in the liver of 47 dairy cows in lactation 2. These samples were collected by means of biopsies performed in the top third of the 12th rib on the right side and the caudal edge of the caudate lobe of the liver, respectively. The herds were composed of crossbred (Holstein x Zebu), kept in extensive production system on Brachiaria brizantha cv Marandu and received mineral supplementation. The mineral mixture in 12 properties were commercial type, called "full" and the owners added a quantity of salt above the manufacturer's recommendations in ten properties. In addition, the mineral mixtures were provided in troughs without covering in seven properties and in other eight, the providing of the mineral mix was not performed daily. In 11 properties there were clinical histories consistent with mineral deficiencies in livestock and placenta retention and osteophagia were the most frequently reported ones. The results of chemical analysis showed deficiency in P in five properties, in Co in two properties, in Se in eight properties, in Zn in eight properties. It is concluded that the deficiency of P, Se and Zn occurs in a greater proportion and the deficiency of Co occurs in a lesser proportion and the mineral supplementation performed in the properties do not supply the daily demands of P, Se and Co, based on the estimated daily consumption of 30 g of NaCl per animal and the troughs little adequate or inadequate for proper supplementation, as well as inconstant supply of mineral mixtures contribute to the deficiency of one or more minerals.Item Acesso aberto (Open Access) Freqüência de anticorpos anti-Borrelia burgdorferi em eqüinos na mesorregião metropolitana de Belém, Estado do Pará(Universidade Federal do Pará, 2006) GALO, Katiany Rocha; FONSECA, Adivaldo Henrique da; http://lattes.cnpq.br/4411441162862608; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557Lyme borreliosis is a multisistemic disease caused by the spirochete Borrelia burgdorferi latu sensu and transmited by ixodide ticks, affecting both humans beings and domesticated animals and having wild animals as Its natural reservoirs, besides of being a zoonosis of wide geographic distribution. We picked 300 blood samples of apparently health horses from the cities of Ananideua, Belém, Benevides, Castanhal, Marituba and Santa Izabel of the Pará, all included in the metropolitan mesoregion of Belém – Pará. The samples were picked up by the jugular vein and the serum analised through indirect Enzyme-Linked Immunosorbent Assay (ELISA) in the Laboratory of Parasitic Diseases of the Federal Rural University of Rio de Janeiro. The aim of this work was to verify the serum epidemiology of Borrelia burgdorferi in the metropolitan mesorregion of Belém. The frequency of serum positivity was 26,67% (n=80), being 72 (24%) with title of 1:800, six (2%) with 1:1600 and two (0,6%) with 1:3200. The serum frequency by gender was approximately 13,67% of females and 13% of males. The thoroughbred and the half-breed animals had 9% and 18% of frequency, respectively. There was no significative difference in the serum positivity with regard to cities, gender, race and age. The frequency found corroborates the hypothesis of occurrence of Borrelia sp. in the studied region.Item Acesso aberto (Open Access) Lesões traumáticas na pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos(Universidade Federal do Pará, 2012-02-24) REIS, Alessandra dos Santos Belo; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557We studied traumatic injury of the skin in horses caused by traumatic plants, popularly known as "sleep-mary", "Poppy", "scraper", "malice" and "do not touch me". The study was conducted on a farm in the district of Castanhal, northeastern of the state of Para, where there were technical visits, epidemiological study, blood samples, biopsies of affected skin and collection of plants. The study included 25 horses, 14 males and 11 females, aged between six months and eight years. The pasture consisted of Brachiaria humidicola and was heavily invaded by traumatizing plants. The animals showed ulcerative lesions of irregular borders, on the head (nose, muzzle, upper and lower lips and chamfer), oral cavity (buccal vestibule and gum) and limbs (billets, metacarpals and metatarsals and scapular-humeral joint). The histopathological examination revealed foci of cutaneous erosions, characterized by loss and epidermal necrosis with spongiosis and vesicular degeneration of the remaining epidermis and mild inflammatory infiltrate in the underlying dermis, consisting predominantly of macrophages and, to a lesser degree, eosinophils. We identified two plants, Mimosa pudica and Mimosa debilis, both from the Leguminosae Mimosoideae family. Based on these results we can conclude that the skin lesions were caused by the traumatic action of Mimosa pudica and Mimosa debilis.Item Acesso aberto (Open Access) Prevalência da anemia infecciosa equina na Ilha de Marajó, estado do Pará, Brasil(Universidade Federal do Pará, 2014) FREITAS, Nayra Fernanda de Queiroz Ramos; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557The aim of the study was to determine the prevalence of equine infectious anemia in the municipalities of Cachoeira do Arari, Salvaterra, Santa Cruz do Arari and Soure, in the Marajó Island, State of Pará, Brazil. For serological survey were selected 349 samples collected from June 2012 to March 2013, tested by agar gel immunodiffusion. Blood count was performed in 65 samples and biochemical examinations in 70, in which was researched urea, creatinine, aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, alkaline phosphatase, total bilirubin and direct bilirubin. A prevalence of 24.06% (84/349) was verified. The average number of red blood cells was significantly lower in seropositive animals in relation to the seronegative ones and there was no significant difference in mean results of hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentration, platelets, in the white blood cell count, as well as in the biochemical examination. The clinical observed was poor nutritional status, apathy, pale mucous membranes, dehydration and elevation in heart and respiratory rate. At necropsy, the primary findings in all horses were jaundiced housing, small accumulation of fluid in the abdominal cavity, as well as hepatomegaly and splenomegaly. The histopathological examination showed spleen and liver with hemosiderosis. The equine infectious anemia is endemic in the cities studied.Item Acesso aberto (Open Access) Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará(Universidade Federal do Pará, 2014-06-26) LIMA, Danillo Henrique da Silva; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557The aim of the study was to estimate the prevalence of Babesia bovis and Babesia bigemina in water buffaloes of the Marajó Island, State of Pará, Brazil. We used an indirect enzyme-linked immunosorbent assay (iELISA), with total antigen containing proteins outer surface, and polymerase chain reaction (qPCR), involving the use of SYBR Green based on amplification of a small fragment of the cytochrome b gene. The prevalence of positive animals in iELISA to B. bovis B. bigemina and mixed infection was 24.87% (199/800), 20.75% (166/800) and 18.75% (150/800), respectively. Using the PCR, the presence of B. bovis was detected in 15% (18/199) and B. bigemina in 16% (19/199) of animals, and of these, 58% (11/19) presented co-infected by the two agents. The results show a low prevalence of an- tibodies anti-B. bovis and anti-B. bigemina in water buffaloes from Marajó Island. However, it was observed that the agents of bovine babesiosis circulate in buffaloes, and these may act as reservoirs.