Dissertações em Ciência Animal (Mestrado) - PPGCAN/Castanhal

URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/2337

O Mestrado em Ciência Animal teve início em 1999 junto à CAPES/MEC e funciona no Programa de Pós-Graduação em Ciência Animal (PPGCAN) do Campus Universitário de Castanhal (CCAST) da Universidade Federal do Pará (UFPA), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) e Universidade Federal Rural da Amazônia (UFRA).

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Acesso aberto (Open Access)
Produção in vitro de embriões bubalinos na ilha do Marajó
(Universidade Federal do Pará, 2022-02-24) RAMOS, Anelise de Sarges; SANTOS, Simone do Socorro Damasceno; http://lattes.cnpq.br/4573238690293498; MIRANDA, Moysés dos Santos; http://lattes.cnpq.br/3354029928888919; https://orcid.org/0000-0001-7771-8174
Aiming to study reproductive biotechniques that can improve in vitro embryo production in the Amazon. The experiment aimed to analyze the effects of rainfall levels, semen used, cryoprotectants (diluters) of semen, presence of the dominant follicle and corpus luteum on the quantity and quality of oocytes and on the rate of embryos The experiment was carried out on the Paraíso farm in the municipality of Cachoeira do Arari, Ilha do Marajó - PA, for one year. Follicles with a diameter greater than 4.0 mm were obtained by the technique of follicular aspiration – Ovum Pick-up (OPU), guided by ultrasound. Forty buffaloes were selected for OPU and three sires for seminal collection, all of the Murrah breed and of reproductive age. For cryopreservation, the BotuBov® and Tes-Tris extenders were tested. The cumulus oophorus complexes (CCOs) were processed in the in vitro production laboratory of the Escola de Ensino Técnico do Estado do Pará (EETEPA). The best ones (CCOs) were matured, fertilized and cultured in vitro, after six days, the embryos were qualified and quantified, using the technique developed by the FIV laboratory at UFPA. The BotuBov® extender was the extender used in the PIVE for presenting better progressive motility (p<0,05) (70,70% ± 10,90%) in relation to the Tes Tris (60,42%±6,00%). There was no statistical difference (p>0,05) in the means and standard deviation of total aspirated follicles (11,75±5,23), of aspirated follicles per animal (11,23±1,39), of total screened COCs (10,5±61,65) and of CCOs tracked per animal (5,96±1,56) between the rainy and less rainy seasons (total follicles aspirated 12,47±4,86, follicles aspirated per animal 11,74 ±1,04, total screened CCOs 118,5±12,02 and screened CCOs per animal 7,27±0,83) respectively. Likewise, there was no significant difference (p>0,05) in the presence of the corpus luteum and dominant follicles and in the body condition score (ECC). In terms of embryos, there was also no statistical difference (p>0,05), between the rainiest (25,25% ±0,64%) and less rainy seasons (19,37% ±14,06%), respectively. We did not find statistical differences in embryo rates (p>0,05) in relation to the semen used (59,33% ±22,27%, 38,29% ±20,35% 54,17% ±31,55%). Thus, we concluded that the variables studied did not influence the production of embryos.
Acesso aberto (Open Access)
Sistema reprodutor feminino de três espécies do gênero Saimiri Voigt, 1831 (Primates: Cebidae): observações macroscópicas e histológicas
(Universidade Federal do Pará, 2014-08-18) LOPES, Gerson Paulino; QUEIROZ, Helder Lima de; http://lattes.cnpq.br/3131281054700225; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149
The platyrrhine primates show variation in their reproductive aspects, their strategies, behaviors, physiology and morphoanatomy. Some of these variations may be consequences of the coevolution of the constituent elements of the genitalia of both sexes through sexual selection. Morphological differences may represent a high degree of specialization of the intraspecific genital organs, which entails a "lock-key" mechanism, which may constitute one of the mechanisms of copulatory and reproductive isolation, with implications in the speciation processes. In females, anatomical and physiological barriers within the vagina, cervix, uterus, uterus-tubal junction, and uterine tubes may be obstacles to male gametes towards fertilization and may influence the outcome of potential sperm competition. In neotropical primates, some morphological descriptions pointed out several similarities and differences between the components of the female reproductive system. Saimiri sciureus, Saimiri oerstedii and Saimiri collinsi were described in primates of the Saimiri genus. Recently, previously identified forms as S. sciureus and considered as subspecies of this, were elevated to the level of species. Among them are Saimiri macrodon and Saimiri cassiquiarensis. These species are widely distributed in the Amazon and occur in peripatria with Saimiri vanzolinii in the Mamirauá Reserve, Central Amazonia. The limits of the geographical distribution of the three species are well delineated, but the mechanisms that promote the reproductive isolation are still not clear. The possibility of hybrids and the progressive invasion of S. cassiquiarensis in the area of S. vanzolinii occurrence are of concern. Some mixed groups were observed in the contact zone between S. vanzolinii and S. cassiquiarensis. The absence of geographical barriers requires that segregation between these species be elucidated by other parameters, such as reproductive isolation due to morphological incompatibility. In this context, we describe the female reproductive system of these species in order to evaluate the possibility that genital morphology constitutes a mechanism of reproductive isolation between them, through the anatomical, topographic and histological description of the external genitalia and internal organs of the female reproductive system. We found that these species share many similarities in most of the organs analyzed. Although some important differences have been identified that may play a relevant role in the evolution of the components of the reproductive system of these species, these differences are not enough to compose a mechanism of reproductive isolation for these three species of Saimiri. Our descriptions provide important information that can assist in the construction of conservation strategies for these and other species of the Saimiri genus. As well as subsidizing the development of reproductive biotechnologies, especially endangered species such as S. vanzolinii, and elucidating questions about evolutionary aspects of the components of the reproductive system of these species and other primates.
Acesso aberto (Open Access)
Produção e características estruturais de capim-Tanzânia sob diferentes frequências de desfolhações em clima tropical Am
(Universidade Federal do Pará, 2016-12-09) MACEDO, Vitor Hugo Maués; CÂNDIDO, Ebson Pereira; http://lattes.cnpq.br/3646672308700905; SILVA, Wilton Ladeira da; http://lattes.cnpq.br/1593390000423230; RÊGO, Aníbal Coutinho do; http://lattes.cnpq.br/4330113577933018
This study determined the effect of different frequency of defoliation (FD) on the structural and production characteristics of Panicum maximum cv. Tanzania during the rainy season (RS) and dry season (DS) of 2015, in the Amazon region of Brazil. A randomized block design with six treatments and five replicates was used. The treatments corresponded to six FD (14; 21; 28; 35; 42 and 49 days). arranged in plots of 12 m2. Were evaluated the demographic pattern of tillering (DPT), tiller population density (TPD), canopy height (CH), leaf area index (LAI), , light interception (LI), total forage accumulation (TFA), and the leaf (LP), stem (SP) and dead material (DMP) proportions. The mean of the variables in each season was subjected to analysis of variance and the unfolding of the interaction between the different FD and the seasons were performed when significant by the F test. The means were analyzed by orthogonal polynomial contrasts using the PROC MIXED procedure of SAS software. Significant interaction (P<0.05) between FD and seasons was observed to the TPD, CH, LI, LAI and TFA variables. The TPD decreased (P < 0.0001) linearly from 891 to 560 tillers/m2 in the RS, and from 533 to 369 tillers/m2 in DS with FD from 14 to 49 days, respectively. Linear response was observed to the CH (P < 0.0001) characterized by the increase of its value with the decreasing of FD. The CH ranged from 58.72 to 117.42 cm with decreasing FD in the RS. In the DS the CH varied less than in the RS wit values between 37.69 and 49.03 cm, characterized for a quadratic adjustment (P = 0.0002). In the RS, the LAI and LI showed quadratic response (P < 0.05) with increasing from 3.60 to 6.05 and from 90.85% to 98.30% in FD from 14 to 49 days, respectively. The TFA which represents the sum of the cycles within each season, showed a quadratic adjustment (P = 0.0028) in RS with higher values for FD of 14 and 21 days, and a quadratic adjustment (P < 0.0001) in DS. In the rainy season it is recommended defoliation frequency around 21 days, corresponding to the 95% light interception and the canopy average height at 71 cm. In the dry season lower frequencies of defoliation are recommended such as every 49 days, due to high forage accumulation in relation to other frequencies in the dry season.
Acesso aberto (Open Access)
Caracterização dos sistemas de produção na bovinocultura leiteira nas mesorregiões sudeste e nordeste paraense
(Universidade Federal do Pará, 2014-02-25) DANTAS, Vanderson Vasconcelos; GODOY, Bruno Spacek; http://lattes.cnpq.br/4036516695601666; OAIGEN, Ricardo Pedroso; http://lattes.cnpq.br/8056365542183068
The dairy industry is of great importance in the regional economy in terms of employment and income generation , especially for family farming . Know the reality of these systems becomes essential so that they can generate specific grants for further development of the activity. In this context, studies on the different production systems emerge as an important tool. The objective of this study systems of milk production was characterized in the regions of Southeast and Northeast Pará through the use of multivariate analysis techniques . The survey was conducted through questionnaires with 112 properties located in the municipalities of Ulianópolis - PA and PA - Irituia . Data were tabulated in Microsoft Excel and processed using SPSS 18.0 software . Through frequency analysis revealed that the total area of varied properties 7-1600 ha . The owners were ranked according to the number of cows in the existing properties and the lowest represented by those with less than 10 cows, which corresponded to 5.35 % and higher over 50 ( 23.21 %). Regarding herd productivity observed an overall average of 4.34 ( L cow -1 day -1) . Feeding the flock is based on cultivated pastures , being the most used braquiarão . Only 19.60% of the properties held supplementation with concentrate and roughage with 13.39% . The reproductive management is performed in 86.6 % through natural mating without any control , which results in little specialized animals for dairy production . The management of milking presents itself as the poor hygienic and sanitary conditions , resulting in a poor quality product . Management practices show little expression among producers . With respect to the typology established through factor analysis revealed four factors that explained 66.99 % of the original variables , and the cluster analysis identified 4 groups of producers with similar profiles . The group III corresponded to the group of producers that had the highest adoption of technology such as supplemental feeding , health management , milking and activity management technology, thus having better performance with an average productivity of 5.02 ( L cow -1 day -1) . Groups I and II are characterized by family production systems with low specialization and low productivity and group IV consists of family farmers who presented with an incipient share of agriculture production , having the lowest production and economic returns.
Acesso aberto (Open Access)
Aspectos da reprodução de espécies de ciclídeos de importância econômica em áreas de várzea do médio solimões
(Universidade Federal do Pará, 2013-02-13) SILVA, Tania Cristiane Gonçalves da; QUEIROZ, Helder Lima de; http://lattes.cnpq.br/3131281054700225
The Cichlidae family plays an important economic role in the Brazilian Amazon, since many of its species are valued as food and also as ornamental fish. The family presents one of the largest parental behaviors among the teleosts, and one of the more diverse among all vertebrates. Amazon fish show a great diversity of reproductive strategies and tactics and ensure the survival of the greatest possible number of offspring, and represent the result of long processes of natural selection and adaptation to seasonal fluctuations of environmental variables. However, many reproductive aspects of this family are still unknown or understudied in the Amazon. This work aimed to study the reproductive ecology of seven species of the family Cichlidae: Aequidens tetramerus, Astronotus ocelatus, Acarichthys heckelli, Acaronia nassa, Hypsellecara temporalis, Mesonauta insignis and Pterophyllum scalare. The study was conducted with specimens collected at Mamirauá Sustainable Development Reserve (MSDR), in the várzea ecosystem, made up of white water flooded environments, and influenced by annual flood pulses. The reserve is a protected area near Tefé, at Amazonas State, Brazil. For each species were evaluated the lengthweight relationship, condition factor, sex ratio, type and spawning period (seasonality), variations of the gonadosomatic relationship, fecundity and size at first sexual maturity (L50). For that, were used 209 specimens of A. tetramerus, 168 of A. ocellatus, 170 of A. heckelli, 178 of A. nassa, 109 of H. temporalis, 380 of M. insignis and 264 of P. scalare. All species showed an isometric growth, with exception of males of A. ocellatus which showed a negative allometric growth. The species bred throughout the year, with spawning activity peaks detected during the rising of the water level, and some additional activity during the flooding period. These were corroborated by RGS and condition factor indices. The overall sex ratio of the species was balanced, 1:1. Except for the species A. nassa, that showed a predominance of males of 0.5:1. All species showed a low fecundity, between 700 and 1500 mature oocytes. The species A. tetramerus, A. ocellatus and H. temporalis showed a partial spawning strategy, and A. nassa, P. scalare and M. insignis showed a total spawning strategy. The asymptotic length (L ∞) and the average length at first sexual maturity (L50) for females showed that all species have an early sexual maturation. The cichlids studied here presented two tactics groups of the main reproductive strategies, K and r. For the management of these species, it should be considered that these are low productivity fish species, and their reproductive seasonal peak and minimum size of capture, based on their L50, should be used to limit the catch.
Acesso aberto (Open Access)
Ocorrência de anticorpos contra os vírus da leucose enzoótica bovina, rinotraqueíte infecciosa bovina e diarreia viral bovina em bubalinos (Bubalus bubalis) no Estado do Pará
(Universidade Federal do Pará, 2014-02-28) MONGER, Suellen da Gama Barbosa; PEREIRA, Washington Luiz Assunção; http://lattes.cnpq.br/9266281009742277
Bovine Leukosis, Bovine Viral Diarrhea and Infectious Bovine Rhinotracheitis are among the main viruses that lead to losses in productivity and reproductive performance of ruminants. Although cattle and buffalo are often created together, and despite the proven occurrence of these diseases in buffalo, papers about natural occurrence of these infections in this species are scarce. Thus, the objective of this study was to verify the occurrence of antibodies against the Bovine Leukosis Virus (BLV), Bovine Viral Diarrhea (BVD) and Infectious Bovine Rhinotracheitis (IBR) in buffalo creations located in the “Paraense” Northeast Mesoregion, Greater Metropolitan Belém and Marajó Mesoregion, Pará, in addition to identify the risk factors for BVD in creations located in “Paraense” Northeast Mesoregion and Greater Metropolitan Belém. In this study 350 buffaloes (Bubalus bubalis) sera were evaluated, coming from farms located in each mesoregion, that were subjected to serological techniques: immunodiffusion for (BLV) and serum neutralizing for BVD and IBR. Frequency of the respective diseases in the study population were: 0% (0/350), 53,71% (188/350) and 91,71% (321/350). Male buffaloes and older animals had higher seropositivity for BVD. The frequency of BVD seroreagents buffaloes was higher in Marajó Mesoregion compared to “Paraense” Northeast Mesoregion and Greater Metropolitan Belém, whereas for IBR the opposite occurred. Among the variables investigated, the type of milking was the only identified as a risk factor for the occurrence of BVD in buffaloes.
Acesso aberto (Open Access)
Utilização de derivado progesterônico na sincronização do ciclo estral em fêmeas bovinas e bubalinas utilizadas na inseminação artificial em tempo fixo (IATF)
(Universidade Federal do Pará, 2012-05-03) NOGUERA GUEVARA, Samuel Enrique; VALE, William Gomes; http://lattes.cnpq.br/7486151987920142
The use of intravaginal devices with progestin as protocol for synchronization of oestrus cycle for fixed time artificial insemination (FTAI) through “Pregna Heat - E'' protocol were used in 219 cows and 169 female buffaloes, belonging to six commercial farms located in the Tachira, Carabobo, and Portuguesa states, in the Bolivarian Republic of Venezuela with the aim to evaluate conception rates (CR) in beef cows and crossbred dairy cattle, milk buffalo and crossbred dairy cows, and crossbred beef and milk heifer as well as in milk buffalo heifers as well as, with the objective to evaluate several variables that can interfere with CR such as body condition score (BCS), number of births, birth interval treatment, degree of difficulty for AI in the farms in the Farm 1, whereas in other farms were evaluated only the CR. The farms adopted a semi-intensive management, with a diet based primarily on forage species introduced in a artificial pasture used in a rotational grazing system with supplementation on the field depending on the needs of each production system. On a Farm 1, located just south of Lake Maracaibo, Tachira State, were synchronized and subjected to FTAI, 22 crossbred dairy cattle heifers, 20 crossbred dairy cows in the postpartum period (PPP) and 18 crossbred buffalo dairy heifers inseminated when 27.2%, 30% and 0 (zero) CR were obtained; moreover in 24 buffalo cows in the PPP synchronized in the outbreeding season (February 2011), it was obtained a CR of 20.8%. For the Farm 2, located in the southern state Tachira, FTAI was performed in favorable season (November 2010) in 78 crossbred dairy buffalo cows in PPP it was obtaining a CR of 34.6%. In the case of Farm 3, located in Urama county in Carabobo State, 49 crossbred buffalo cows in the PPP, were subjected to FTAI when it was obtaining a CR of 42.8%; in Farm 4, located in Santa Ana county, Tachira state, 85 crossbred dairy cattle cows were used for FTAI with 40% CR obtained, whereas in Farm 5, located in Portuguesa state, 23 crossbred beef cows of dual purpose in PPP were subjected to FTAI and it was found47.8% CR; finally in the Farm 6 located in Portuguesa State 20 crossbred beef heifers and 49 crossbred beef cow, were subjected to FTAI were found a CR of 55 and 48.8%, respectively. Also on Farm 1 were compared variables such as CR and number of births, when it was clear demonstrated that CR in pluriparous beef cows with more than four parturitions the results obtained where quite higher when confronted with cows with 2-3 parturitions and primiparous (40.5 ≥ 4 births, 28.5% 2-3 births and 0% for primiparous), (P = 0.4130); when compared with CR and parturition interval and the use of FTAI protocol “Pregna Heat - E'', the CR is higher in cows between 60 and 200 days postpartum, than cows with the interval of more than 200 days, which showed a very low CR (9.0%) (P = 0.0241); regarding to ECC no significant statistical difference important were observed among females with BSC=3 submitted to FTAI when confronted to animals with higher BCS. In the case of Farm 3, located in Urama county in Carabobo State, 49 crossbred buffalo cows in the PPP, were subjected to FTAI obtaining a CR of 42.8%, whereas in Farm 4, located in Santa Ana county, Táchira state, 85 crossbred dairy cattle cows was used for FTAI, 40% CR obtained, and in Farm 5, located in Portuguesa state, where 23 crossbred beef cows of dual purpose in PPP subjected to FTAI, it was found 47.8% CR; finally in the Farm 6 located in Portuguesa State 20 crossbred beef heifers and 49 crossbred beef cow, were subjected to FTAI when was found a CR of 55 and 48.8%, respectively. Still in the Farm 1 were compared variables such as CR and number of births, when it was clear demonstrated that CR in pluriparous beef cows with more than four parturitions where quite higher when confronted with cows with 2-3 parturitions and primiparous (40.5 ≥ 4 births, 28.5% 2-3 births and 0% for primiparous), (P = 0.4130); when compared with CR and parturition interval and the use of FTAI protocol “Heat-pregna - E'' the CR is higher in cows between 60 and 200 days postpartum, than cows with the interval of more than 200 days, which showed a very low CR (9.0%) (P = 0.0241); regarding to ECC no significant statistical difference important were observed among females with BSC=3 submitted to FTAI when confronted to animals with higher BCS showing a very poor (P=0.1632). With reference to the degree of difficulty for AI, animals with 0 degree of difficulty for AI, presenting the highest conception rates compared to the animals of 1 degree (44.4 and 31.2%), respectively. The use of the protocol “Pregna Heat - E” for FTAI, can be recommended for both in cattle and buffaloes with satisfactory results, however the CR in the case of crossbred Bos taurus or crossed animals with more blood of this sub-species probably due the fact that are raised in hot and humid regions as in the case of Farm 1, it has been observed a decrease in CR, which can be attributed to environmental factors interfering negatively on this variable. Likewise it is important to note that female buffaloes must be synchronized at the favorable season, not in outbreeding season, since throughout this time there was a decrease in CR in buffaloes submitted to FTAI, 20.8 vs 34.6% and 42.8%, respectively. In the case of the use of synchronization protocols such as “Pregna Heat – E” for FTAI use in buffalo heifers in the present study showed to be inadequate, with zero of CR, probably due anatomical and physiological concerned to the reproductive tract (very small cervix), thus buffalo heifers should not be included in FTAI programs with conventional techniques. The protocol ''Pregna Heat - E” can be used as a viable tool for oestrus cycle synchronization and FTAI in both cattle and buffaloes, and in the present study with the bovine CR being higher than in buffaloes (40.1 vs 31, 9%) (Fisher = 0.9624, P = 0.0212, P <0.05). That being so the use of “Pregna Heat - E” protocol as was used in the present study needs to be further studied in buffalo to obtain higher conception rates.
Acesso aberto (Open Access)
O Caranguejo-Uçá, Ucides cordatus, (Linnaeus 1763): da captura à comercialização nas comunidades quilombolas cacau e terra amarela, Ilha de Colares, Pará-Brasil
(Universidade Federal do Pará, 2014-07-10) CARVALHO, Rogério Lopes; QUEIROZ, Helder Lima de; http://lattes.cnpq.br/3131281054700225
This study was conducted at the quilombola communities of Cacau and Terra Amarela, municipality of Colares, Pará State, Brazil. The objective was to describe and analyze the exploitation of the land crab, or mangrove crab (Ucides cordadus), in terms of some socioeconomic aspects, their techniques, strategies and areas of production adopted, and their forms of crab trade. And to compare these aspects with those from other traditional populations also dedicated to the same extractive activity. Semi-open questionnaires, direct observation in the field and trade and flowchart, were techniques applied in this research during 2013. In the communities studied, the extractive economy is predominant, and mangrove crab extraction is among the main activities. At Cacau and Terra Amarela, households have in average 4.3 and 4.9 members, respectively. Most of the families live in their own brickwork houses, built with conventional materials. Despite of this, these extractive households are poor and live in difficult conditions, with almost no access to public services. In general, the inhabitants stop attending school early in their lives, and most of them (72 to 82%) failed to complete the elementary school cycle. A low monthly household income was recorded; the majority of the households earn less than one minimum salary per month. The laço and the braceamento are the main techniques for obtaining the resource. The technique of braceamento is performed during the four days of work per week in average during the rainy months. The application of laço is performed in average during twelve days per month during the drought months. Cacau produces in average 52 (SD ± 15) crabs / work day / person and 110 (SD ± 56) crabs / work day / person in in the rainy and drought periods, respectively. In the Terra Amarela community these averages are 56 (SD ± 24) crabs / day / person and 150 (SD ± 72) crabs / day/ person. The inhabitants of these communities trade with intermediaries from the region. The crabs are sold in natura, whole and alive, transported in cofos in Cacau and bags in Terra Amarela. Are traded at prices that fluctuate throughout the year, from R$ 40.00 to R$ 80.00 each group of 100 individuals. The low pay for they work, together with low education level, deficiencies in infrastructure, low technology for production, and lack of local provision of public services are some of the aspects contributing to the low quality of life of these families in the communities. These factors also contribute for the maintenance of the engagement of local inhabitants in low value activities, such as the extraction of mangrove crabs. The conditions described for Cacau and Terra Amarela are quite similar to those observed in other traditional communities living and working in similar conditions in the coastal region of Pará State, Brazil. The extraction and trade are very similar to most of the traditional communities that exploit this resource in mangroves forests along the coast of Pará. No significant differences or peculiarities were observed in the extractive activity of these quilombola communities, in relation to other traditional populations performing this same activity in the area. However, differences were found between the two communities studied, which represent variations of this general pattern identified. Probably these differences are related to the technologies adopted, and also on the amount of crabs captured in the two locations. These impact the costs of the activity, its profitability, and the quality of life of inhabitants of Cacau and Terra Amarela.
Acesso aberto (Open Access)
Localização do receptor de melatonina Mel1a e da enzima NRH: quinona redutase 2 em embrião e retinas inteiros de Kinosternon scorpioides
(Universidade Federal do Pará, 2014-06-27) SILVA, Renata Nunes; SAMPAIO, Lucia de Fatima Sobral; http://lattes.cnpq.br/7145234400169550
The pineal and others tissues produced hormone melatonin synchronizes biological clocks with dark light environmental cycle. This hormone has functions on development via Mel1a melatonin receptor and NRH: quinona redutase 2 (QR2) enzyme. The present aim was to initiate an investigation about the localization of the both Mel1a melatonin receptor and QR2 enzyme melatonin binding site in vertebrate development. We taken as a model the fresh water turtle Kinosternon scorpioides (muçuã). Whole 21 days embryos (E21) and retinas from E21 and 60 days posthatched (PH) muçuãs respectively were assayed by immunoassays and immunohistochemistry, using commercial antibodies and the results were revealed by Texas red fluorescence. As Mel1a melatonin receptor as QR2 enzyme were localized in important external morphological characters in E21 and in retinas from both E21 and PH. Results showed Mel1a melatonin receptor in maxillary, mandibula, eye contour, Iris, choroide fissure, neck, elongated forelimb and hindlimb, developing carapace, beyond of the internal part of the tail bud. QR2 fluorescent signals in whole E21 embryos were in the following morphological external characters. In head, QR2 is at maxillary region; in the eye at lens and iris; in elongated forelimb and hindlimb; and in tail bud. No fluorescence was observed in negative controls incubated without the primary antibody. In conclusion, our achievers suggest that melatonin participates in Kinosternon scorpioides development as in ossification, hole of the Mella receptor, as in xenobiotic protection, hole of the QR2 enzyme. Melatonin functions on turtle development disclose are only starting, but we found some interesting answers and future investigations arise from these studies.
Acesso aberto (Open Access)
Composição e distribuição espaço-temporal das larvas de peixes do estuário do Rio Quatipuru - Nordeste Paraense
(Universidade Federal do Pará, 2011-10-25) SILVA, Potira Fernandes e; MELO, Nuno Filipe Alves Correia de; http://lattes.cnpq.br/4989238044542736; QUEIROZ, Helder Lima de; http://lattes.cnpq.br/3131281054700225
In Quatipuru Estuary, located in Northeast Pará two campaigns were carried out in dry season (November/2009) and rainy season (May/2010) with spatial and nictemeral collections. Larvae were collected using a plankton net, conical, with 60 cm mouth opening, 1.80 m long and 500 m mesh opening. The samples were screened and identified as to their stage of larval development and species level, some typed. The family Clupeidae was the most abundant in drags spatial-temporal in dry season and Scianidae (Cynoscion acoupa) characterized the rainy season. In dry season, were observed low density, highest value 8.50 ind./m³ (Clupeidae sp.3) and the lowest value 0.06 ind./m³ (Engraulididae sp.2). In the Quatipuru Estuary 65% of the larvae at the stage of pre-flexion, flexion 25% and 10% post-flexion. In rainy season were observed 64% in pre-flexion, flexion 14% and 22% in post-flexion. In cluster analysis (mode Q) distinguished two main groups according to the seasonal site, so R did not distinguish between major groups and the association of species. According Principal Component Analysis (PCA) the first two axes explained 82% of data variance, a trend can be observed according to the distribution of species richness. In nictemeral drag, the Clupeidae family was mean larval density in dry season (Clupeidae sp.1), and Scianidae family best characterized in rainy season (Cynoscion acoupa). Also very low density, the higher value of 2.96 ind./m³ during the rainy season and the lowest 0.01 ind./m³ in the dry season. During the dry season were 57% in the stage of preflexion, 26% flexion and 17% in post-flexion, since the rainy season 11% in pre-flexion, 38% flexion and 51% in post-flexion. The Q mode cluster analysis also distinguishes two groups according to seasonality (dry/rainy), the R mode also did not distinguish between major groups regarding the association of its kind. In PCA. the shaft 01 is responsible for 70% directly related to the richness and density of organisms, a tendency was observed of distribution according to species occurrence in the horizontal direction.
Acesso aberto (Open Access)
Caracterização do sistema produtivo da aquicultura da microrregião de Cametá – Pará
(Universidade Federal do Pará, 2013) FERREIRA, Palmira Francisca Gonçalves; FUJIMOTO, Rodrigo Yudi; http://lattes.cnpq.br/9538142371454660
This research is aimed to present a diagnosis of aquaculture activity in the micro region of Cametá - Pará, emphasizing the factors that influence the development of activity and the level of competitiveness through the Competitive Performance Index (CPI) in order to provide indicators that can facilitate decision making in the areas of promoting aquaculture establishment as an economic activity. Data collection was performed by using questionnaires that sought to investigate the aspects relevant to productive activity. The data was processed and analyzed using the statistical SPSS software, version 13. This software made it possible to obtain a reduced linear structure of the original data set, through the summarization of the information contained in the variables creating smaller variable sets lalled factors, to identify the Competitive Performance Index (CPI) with respect to the variables studied. The results showed that 70.50% of the region's farmers have a primary education and only 1.44% attended or initiated higher education. 91,37% of the employees are members of the farm owners family. The average area intended for cultivation was 500 m2. The largest was found in Oeiras do Pará, which is also the largest municipality in area. The means of production include the creation of ponds in lowland areas (78.05%), in net-cage 13,3% and conventional production system with 8,65%. The prevalent mode of cultivation is the monoculture, especially the round fish tambaqui and tambacu (54%). Factor analysis extracted four factors representative of the development in the region: the productive system dimension, which explained about 27% of the total variance; commercial dimension, accounting for 15.15%; efficiencydimension explained 13.3% of the total and work dimension that explained 12.5% of the total variance. These factors influence the development of activity and should direct the actions of rural entrepreneurs and policy with the aim of improving fish farms so that they can boost production and meet growing demands for food quality.
Acesso aberto (Open Access)
Prevalência da anemia infecciosa equina na Ilha de Marajó, estado do Pará, Brasil
(Universidade Federal do Pará, 2014) FREITAS, Nayra Fernanda de Queiroz Ramos; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557
The aim of the study was to determine the prevalence of equine infectious anemia in the municipalities of Cachoeira do Arari, Salvaterra, Santa Cruz do Arari and Soure, in the Marajó Island, State of Pará, Brazil. For serological survey were selected 349 samples collected from June 2012 to March 2013, tested by agar gel immunodiffusion. Blood count was performed in 65 samples and biochemical examinations in 70, in which was researched urea, creatinine, aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, alkaline phosphatase, total bilirubin and direct bilirubin. A prevalence of 24.06% (84/349) was verified. The average number of red blood cells was significantly lower in seropositive animals in relation to the seronegative ones and there was no significant difference in mean results of hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentration, platelets, in the white blood cell count, as well as in the biochemical examination. The clinical observed was poor nutritional status, apathy, pale mucous membranes, dehydration and elevation in heart and respiratory rate. At necropsy, the primary findings in all horses were jaundiced housing, small accumulation of fluid in the abdominal cavity, as well as hepatomegaly and splenomegaly. The histopathological examination showed spleen and liver with hemosiderosis. The equine infectious anemia is endemic in the cities studied.
Acesso aberto (Open Access)
Determinação da ocorrência de anticorpos anti-Leptospira spp. em búfalos (Bubalus bubalis) criados no bioma Amazônico Paraense
(Universidade Federal do Pará, 2015-12-04) LEAL, Mario Arthur da Costa; SALVARANI, Felipe Masiero; http://lattes.cnpq.br/3339195791140438
This study investigated the occurrence of Leptospira spp. in buffaloes in the Amazon biome of Pará, as well as identify the higher incidence of serotypes in the region. They tested 387 serum samples of buffalo nine Pará municipalities, Cachoeira do Arari, Santa Cruz do Arari, Salvaterra, Soure and Chaves located on Marajó Island, Abaetetuba, Ipixuna, Nova Timboteua and Paragominas, located on the mainland. Eight serotypes were tested: Bataviae, Bratislava, Hardjobovis, Hardjo (OMS), Hardjo (CTG), Icterohaemorrhagiae, Pomona and Hardjo (Bolivia), through the microscopic agglutination test method (SAM). Of the total samples tested 354 (91.50%) were reactive serum for at least one serovar and 33 (8.50%) were negative. Three serotypes of serogroup Sejroe were more frequent, Hardjo (Bolivia) (79.30%), Hardjo (OMS) (64.8%) and Hardjobovis (64.10%). It is important to know the higher incidence of serotypes in the region studied so that the directions of combat and infection control are efficient, minimizing the potential risk of contamination and maintenance of leptospirosis in the herd.
Acesso aberto (Open Access)
Expressão gênica e viabilidade de folículos pré-antrais submetidos à vitrificação do córtex ovariano de Sapajus apella (macacas-prego)
(Universidade Federal do Pará, 2012-05-25) SANTANA, Luana de Nazaré da Silva; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149; SANTOS, Regiane Rodrigues dos; http://lattes.cnpq.br/0500967766886604
Vitrification is a biotech that has been increasingly showing promise in several species, among them domestic ruminants (such as sheep, goats and cows) and in nonhuman primates (NHP) (as cynomologus and rhesus), and consists in reducing ultra-rapid temperature by the presence of high concentrations of cryoprotective agents (PCA's) in liquid nitrogen. Thus the aim of this study was to develop a methodology for cryopreservation by vitrification of preantral follicles (PF's) in Sapajus apella. For this purpose, we used females (n = 9) adult Sapajus apella squad belonging to the National Primate Center (CENP). Samples were taken from the ovarian cortex by laparotomy so as not to destabilize the animals reproducibly. The fragments exposed to 8 different treatments: Ethylene glycol (EG) + 40% Sucrose (Sac) dissolved in 0.5 M TCM-199, added to Selenium (2,5, 5 or 10 ng / ml) or Trolox (25, 50 or 100 mM). Following exposure to cryoprotective agents was analyzed follicular viability before and after vitrification, from the follicular morphology, expression of Hsp70 genes, Erp29, Erp60 SOD1 and through the analysis by Polymerase Chain Reaction (PCR), in addition to performing measurement of oxidative stress thru the TEAC (English Total Equivalent Antioxidant Activity). The analyzes showed that vitrification allowed the maintenance of follicular viability by previous exposure to concentrations of ACP's alevadas, especially when supplemented with 50 mM trolox (which resulted in high follicular survival rates) and increased expression of the antioxidant enzyme SOD1. While in the absence of it was observed an increase in rates of follicles degenerate and vacuolated, and reduced expression of the antioxidant enzyme SOD1 and increased expression of chaperone Erp29.
Acesso aberto (Open Access)
Cadeia produtiva da bovinocultura leiteira no município de Tailândia, estado do Pará: sistemas de produção e tecnologia
(Universidade Federal do Pará, 2015) NERES, Lilaine de Sousa; SENA, Ana Laura dos Santos; http://lattes.cnpq.br/6603890343726711; LOURENÇO JÚNIOR, José de Brito; http://lattes.cnpq.br/2919433679918544
This dissertation aims to characterize the productive chain of dairy cattle in the municipality of Thailand, Pará, Mesoregion Northeast Pará. Were evaluated secondary data on government and institutional sites, and primary data through interviews with producers, community leaders and experts from public institutions. The milk production chain suffered changes due to changes in the local economy, characterized by pressure on environmental enforcement and introduction of new crops. The median age of producers was 52.5 years, with low educational level and performance in the business for over ten years. Milk production is the main income-generating activity, among other rural and urban character. The properties using traditional system with reduced use of forages and supplemental feeding. As health measures, there is compliance on the implementation of FMD vaccine, under control and supervision of the Agricultural Protection Agency of Pará, as well as prevention against parasites and worms, however, there is no control and reproductive planning. Milking is done manually in a covered corral, once a day, and only 34.7% of the properties have piped water on site to hygiene of food handlers, utensils and animals. Because of the suspension of activities of the municipal dairythe production milk and dairy products is piped to the informal trade, which configures a public health problem. The properties had a high technological level, reflecting in animal productivity, with an overall average of just 4.59 liters / cow / day. In this context, it is important that public policies promote sustainable production systems through technology transfer, the development of production of dairy products, with certification of quality and food safety, bearing in mind the preservation of social, economic cultural and of family farmers.
Acesso aberto (Open Access)
Caracterização estrutural e ultraestrutural das células do epitélio gonadal de Hypophthalmus Marginatus (Valenciennes 1840) durante a espermatogênese
(Universidade Federal do Pará, 2014-09-05) GONÇALVES, Liziane Amaral Barbosa; ROCHA, Rossineide Martins da; http://lattes.cnpq.br/4371300451793081
Hypophthalmus marginatus is a siluriforme, belonging to the family Pimelodidae. This species is highly consumed in eastern Amazonia and has a wide socio-economic importance to the region. Studies on the histological characterization of the reproductive system are still unknown in this species. Therefore, this study serves as a tool for biotechnology techniques, necessary for the production in captivity, since this species is one of the fishing resource most intensively exploited by artisanal fisheries. The aim of this study was to characterize the structural and ultrastructural features of germinal epithelium cells of Hypophthalmus marginatus during spermatogenesis. 168 specimens were collected from December 2011 to December 2012. Macroscopically were identified 69 adult males. In field, ventral incision was held in fishes and fragments of testicles from the median region and semen were removed. Then the material was subjected to processing and analysis of light microscopy, transmission electron microscopy and scanning. The data revealed that the germ cells proliferate inside the spermatic cyst synchronously. Spermatids enter at the spermiogenesis process through three distinct stages of cell differentiation. The nucleus does not move relative to the axis flagellar and the centrioles don't migrate to the nucleus. The spermatozoids showed a spherical head with the highly condensed nucleus and no acrosome. The intermediary piece was short containing a single long flagellum. The axoneme presents the structure of microtubules within the pattern (9 + 2). The nuclear fossa housed the proximal centriole partially. The presence of spermatogenic cells in the same subphase of meiosis inside the cyst is characterized as cystic spermatogenesis, with synchronous growth of the cells. This suggests that spermiogenesis of H. marginatus is of type III and the presence of nuclear fossa is considered a distinctive feature for the species, which differs from other pimelodids.
Acesso aberto (Open Access)
Ocorrência de micoplasmas hemotrópicos em caninos e felinos domésticos na região de Belém, PA
(Universidade Federal do Pará, 2014-02-07) MORAES, Leopoldo Augusto; GONÇALVES, Evonnildo Costa; http://lattes.cnpq.br/8652560763793265; PEREIRA, Washington Luiz Assunção; http://lattes.cnpq.br/9266281009742277
Hemotrophic mycoplasmas comprise a group of bacteria that can cause hemolytic anemia and several chronic diseases in animals. In cats three infecting species of hemoplasmas are reported: M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, the first being considered the most pathogenic, whereas the dogs are normally infected by at least two mycoplasma species M. haemocanis and ‘Candidatus M. haematoparvum’. In order to assess the occurrence of hemotrophic mycoplasmas in feline and canine populations of Belém city were analyzed, by PCR, the genetic material from blood samples of 299 domiciled and stray animals. In 8.03% (24/299) of DNA samples of hemotrophic mycoplasmas was amplified, 1.78 % (3 /169) from infected dogs and 16.15% (21 /130) from infected cats. Among the cats the highest rates of infection were found in domiciled animals 22.86% (16/70) and in males 29.51% (18/61), the species ‘Candidatus M. haemominutum’ 8.46 % (11/130) get the higher incidence when compared to species Mycoplasma haemofelis 6.92 % (9/130). The infection in dogs was only identified among stray animals, with the ‘Candidatus M. haematoparvum’ being the only species identified with a rate of 1.78 % (3/169). It was possible to assess that hemotrophic mycoplasmas circulate in both species in the present report, domiciled felines and males formed the group at highest risk for infection and the agent ‘Candidatus M. haematoparvum’ seems to be the only one in circulation in the canine population.
Acesso aberto (Open Access)
Biometria e barimetria da tartaruga da Amazônia, Podocnemis expansa (SCHWEIGGER, 1812), em sistemas comerciais
(Universidade Federal do Pará, 2008) PRINTES, Lanna Daniella dos Santos; ARAÚJO, Cláudio Vieira de; http://lattes.cnpq.br/5049897507837031
The Chelonians represent one of the main bilological resourses of the Amazon region and stand as a important food source for the people that live by the rivers. For that reason, the possibility of commercial rising came forth. Biometries have been made to develop barometric equations in order to determinate the biometry of the amazon turtle and evaluate functions that can be.used for the barometry of the animals at two commercial rinsing fields. Living weight records was used separate heavy and light samples to each site. Because the great variation of the living weight, it was converted to logarithmic scale. To compare two different levels of years and sites, a multivariated variance analysis was performed. A discriminating analysis was adopted by using a canonic variable. The great living weight variation in each year and site reveals that in samples with same age and origin, the animals show great heterogeneity for living weight. The result of the canonic correlation for two rising fields indicates that the living weight can be explained in 86% and 96% when a linear relation is established. Excepting the head width and tail length variables, all others demonstrated highly correlated to the living weight. Therefore, there are a high variation of the living weight among animals created in captivity. The biometries are highly related to the living weight and should be included in studies that seek barometry.
Acesso aberto (Open Access)
Encefalomielite equina Leste na Ilha de Marajó, Pará
(Universidade Federal do Pará, 2012-02-29) CAMPOS, Karinny Ferreira; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557
Nine cases of equine encephalomyelitis were studied in the Marajó Island, State of Pará, Brazil. The animals had difficulty in maintaining a station, walk in a circle, marked depression, eyelids closed, tongue paralysis, muscle tremors, bruxism, anorexia and dehydration. Some had their ear and eyelid reflexes diminished, decreased tongue tone and tachycardia. Position of self-hearing was observed frequently. The animals were often found leaning on tree trunks and fences to keep themselves on station. At necropsy, they showed hemorrhage of the meninges and spinal cord, and some animals also showed adhesion of the meninges. Histologically there was diffuse encephalitis affecting mainly the gray matter, with meningitis and choroiditis. It was observed the presence of perivascular cuffs consisting of mononuclear inflammatory cells. In two animals it was possible to identificate the Eastern equine encephalitis virus by semi-nested reverse transcription polymerase chain reaction (semi-nested RT-PCR).
Acesso aberto (Open Access)
Manejo de Scaptotrigona Sp. (Hymenoptera, Apidae, Meliponini) para polinização da rambuteira (Nephelium Lappaceum L.)
(Universidade Federal do Pará, 2014-07-31) LEÃO, Kamila de Sousa; CONTRERA, Felipe Andrés León; http://lattes.cnpq.br/3815182976544230; VENTURIERI, Giorgio Cristino; http://lattes.cnpq.br/7180149611727426
Meliponini bees can be easily kept and they are important crop pollinators. This study aimed to manage a Scaptotrigona species to improve pollination in rambutan trees (Nephelium lappaceum L.). The first chapter analysed the phenological development (flowering and fruit phases) in rambutan crop. Two blossom periods per year were identified. The average cycle duration (from anthesis to harvest fruit point) was 123-128 days. The objective of the second chapter was evaluate the effect of Scaptotrigona sp. nests in rambutan fruit set. There were not significantly differences on rambutan fruit set upon bee presence or absence, although Scaptotrigona sp. presented high index of pollen fidelity to rambutan. The last chapter focused on a specific question about stingless beekeeping. It was tested whether a rational hive model (Embrapa) was better than a traditional hive (cabocla) for Scaptotrigona sp. management, by evaluating certain aspects, such as biological adaptation and management, . It was found that the studied hive (Embrapa) is a suitable model to rational creation of Scaptotrigona.