Dissertações em Ciência Animal (Mestrado) - PPGCAN/Castanhal
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/2337
O Mestrado em Ciência Animal teve início em 1999 junto à CAPES/MEC e funciona no Programa de Pós-Graduação em Ciência Animal (PPGCAN) do Campus Universitário de Castanhal (CCAST) da Universidade Federal do Pará (UFPA), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) e Universidade Federal Rural da Amazônia (UFRA).
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Navegando Dissertações em Ciência Animal (Mestrado) - PPGCAN/Castanhal por Orientadores "DOMINGUES, Sheyla Farhayldes Souza"
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Item Acesso aberto (Open Access) Criopreservação do sêmen de macaco-prego (Sapajus apella Linnaeus, 1758): avaliação de diferentes diluidores, concentração de glicerol e antioxidante catalase(Universidade Federal do Pará, 2015-02-24) LEÃO, Danuza Leite; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149The main objective of the present study was to establish an efficient semen cryopreservation protocol in Sapajus apella for maintaining sperm viability. For this, we compared the performance of TES-TRIS, CWS and ACP-118® extenders during the seminal coagulum dissolution and cooling. Then, in order to improve the sperm parameters, we also determined the glycerol concentration (3; 5 and 7%) as well as to evaluate the effect of catalase antioxidant (10 µg and 50 µg). Therefore, six adult males of S. apella from the National Primate Center were used. Semen was collected by electro-ejaculation with rectal probe after chemical restraint of animals with ketamine and xylazine hydrochloride. In the first phase of the project, the semen obtained was diluted in TES- TRIS, CWS and ACP®-118 extenders, kept in a water bath at 37 °C. After coagulum dissolution, the semen was cooled in the refrigerator at 4 °C for 90 minutes and evaluated after 28h for motility, vigor and plasma membrane integrity percentage before and after the cooling. ACP®-118 was the best extender to preserve the motility sperm and plasma membrane integrity after 28 hours of incubation. Based on these results, cryopreservation was performed by evaluation of different concentrations of glycerol (3, 5, and 7%). The cryopreserved sperm to 3% glycerol had better results. In the second project phase was performed S. apella semen cryopreservation in ACP®-118 extender supplemented or not with catalase antioxidant (10 µg/mL and 50 µg /mL). All treatments were effective in maintaining sperm parameters and was possible to recovery the post-thaw motility. The catalase 50 treatment was the best for maintenance of the vigor after cooling and plasma membrane integrity in post-thaw sperm. So, we concluded that ACP-118® extender can be efficiently used for S. apella semen cryopreservation added 3% glycerol, moreover, the addition of the catalase antioxidant showed beneficial effect during this process.Item Acesso aberto (Open Access) Expressão gênica e viabilidade de folículos pré-antrais inclusos em fragmentos de córtex ovarianos cultivados in vitro de macaco-prego (Sapajus apella)(Universidade Federal do Pará, 2011) BRITO, Adriel Behn de; SANTOS, Regiane Rodrigues dos; http://lattes.cnpq.br/0500967766886604; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149The aim of the present study was to investigate the stability of three reference genes in the ovarian tissue of capuchin monkeys (Sapajus apella) and to develop a short-term in vitro culture system for the activation and growth of preantral follicles from capuchin monkeys. To this end two experiment were conducted as follow. Experiment I: Fresh and cryoprotectant exposed ovarian biopsies were used. Both fresh and exposed ovarian tissues were subjected to total RNA extraction and synthesis of cDNA. After amplification of cDNA by real-time PCR, the GeNorm, Bestkeeper and Normfinder software were used to evaluate the stability of glyceraldehyde-2-phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT1) and TATA-binding protein (TBP). Experiment II: Ovarian tissue from four healthy mature females were collected and divided into nine ovarian cortical pieces of 1 mm³. One ovarian fragment (control) was immediately divided in two pieces, which were subjected to viability analysis or qRT-PCR. The remaining 8 fragments were individually cultured in vitro in a medium consisting of TCM supplemented with 100 ng/mL EGF (T1), either or not added with 10 μM BME (T2), 100 ng/mL BMP4 (T3), 25 IU PMSG (T4), 10 μM BME and 100 ng/mL BMP4 (T5), 10 μM BME, 25 IU PMSG (T6), 100 ng/mL BMP4, 25 IU PMSG (T7) or 10 μM BME, 100 ng/mL BMP4, 25 IU PMSG (T8). Results demonstrated that, in the ovarian tissue from capuchin monkeys, HPRT1 and TBP were the most suitable reference genes and thus could be used as parameters to normalize data in future studies. In contrast, GAPDH appeared as the least stable gene among the tested reference genes. After in vitro culture, all treatments resulted in similar percentages of viable preantral follicles. Ovarian tissue cultured in the presence of EGF + BME/BMP4/PMSG resulted in an increased rate of follicular activation and growth, as well as in the up regulation of AMH, BMP15 and GDF9, specific markers of follicular development. In conclusion, HPRT1 and TBP are the most stable reference genes in fresh and cryoprotectant exposed ovarian tissue from capuchin monkeys. Preantral follicles are able to develop in vitro when cultured in a medium supplemented with PMSG, BME and BMP4. Follicular viability, however, was maintained independently on the culture medium. The use of growth factors as markers of follicular development was crucial to identify the best culture medium.Item Acesso aberto (Open Access) Sistema reprodutor feminino de três espécies do gênero Saimiri Voigt, 1831 (Primates: Cebidae): observações macroscópicas e histológicas(Universidade Federal do Pará, 2014-08-18) LOPES, Gerson Paulino; QUEIROZ, Helder Lima de; http://lattes.cnpq.br/3131281054700225; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149The platyrrhine primates show variation in their reproductive aspects, their strategies, behaviors, physiology and morphoanatomy. Some of these variations may be consequences of the coevolution of the constituent elements of the genitalia of both sexes through sexual selection. Morphological differences may represent a high degree of specialization of the intraspecific genital organs, which entails a "lock-key" mechanism, which may constitute one of the mechanisms of copulatory and reproductive isolation, with implications in the speciation processes. In females, anatomical and physiological barriers within the vagina, cervix, uterus, uterus-tubal junction, and uterine tubes may be obstacles to male gametes towards fertilization and may influence the outcome of potential sperm competition. In neotropical primates, some morphological descriptions pointed out several similarities and differences between the components of the female reproductive system. Saimiri sciureus, Saimiri oerstedii and Saimiri collinsi were described in primates of the Saimiri genus. Recently, previously identified forms as S. sciureus and considered as subspecies of this, were elevated to the level of species. Among them are Saimiri macrodon and Saimiri cassiquiarensis. These species are widely distributed in the Amazon and occur in peripatria with Saimiri vanzolinii in the Mamirauá Reserve, Central Amazonia. The limits of the geographical distribution of the three species are well delineated, but the mechanisms that promote the reproductive isolation are still not clear. The possibility of hybrids and the progressive invasion of S. cassiquiarensis in the area of S. vanzolinii occurrence are of concern. Some mixed groups were observed in the contact zone between S. vanzolinii and S. cassiquiarensis. The absence of geographical barriers requires that segregation between these species be elucidated by other parameters, such as reproductive isolation due to morphological incompatibility. In this context, we describe the female reproductive system of these species in order to evaluate the possibility that genital morphology constitutes a mechanism of reproductive isolation between them, through the anatomical, topographic and histological description of the external genitalia and internal organs of the female reproductive system. We found that these species share many similarities in most of the organs analyzed. Although some important differences have been identified that may play a relevant role in the evolution of the components of the reproductive system of these species, these differences are not enough to compose a mechanism of reproductive isolation for these three species of Saimiri. Our descriptions provide important information that can assist in the construction of conservation strategies for these and other species of the Saimiri genus. As well as subsidizing the development of reproductive biotechnologies, especially endangered species such as S. vanzolinii, and elucidating questions about evolutionary aspects of the components of the reproductive system of these species and other primates.