Dissertações em Neurociências e Biologia Celular (Mestrado) - PPGNBC/ICB

URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/2375

O Mestrado Acadêmico pertence ao Programa de Pós-Graduação em Neurociências e Biologia Celular (PPGNBC) do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA).

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Agora exibindo 1 - 14 de 14

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Análise citogenética de profissionais de serviços de radiologia clínica expostos à radiação ionizante na cidade de Belém, Pará, Brasil
(Universidade Federal do Pará, 2011-05-06) CUNHA JUNIOR, Luiz Raimundo Campos da Silva e; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Acesso aberto (Open Access)
Análise imunológica e genotóxica em Rattus Novergicus da linhagem wistar tratados com ciclofosfamida
(Universidade Federal do Pará, 2016-08-11) CARVALHO, Heleniana Maria Miranda de; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
The development of this work has given up due to the need to better understand the immune system, taking into account the diversity of experimental immunosuppression models as well as the variety of immunological responses and genotoxic differences these, related species, the drug and doses used. Thus, aim of this study was to analyze the effects on the immune system and genotoxic effects in Rattus norvegicus Wistar, after inoculation of the alkylating agent cyclophosphamide (CY). The administration of 50 mg / kg in rodents CY, possible to observe a significant decrease in the parameters of cellularity and relative weight of lymphoid organs. The humoral immunity of rodents has undergone deletion, since the analysis of the antibody titration was performed on the test plate forming cells and hemolysis testing. four inoculations that immunosuppressant and the intervals between the inoculations was determined by recovery of normal levels of the above parameters were performed. Both times the drug was administered, there was a reduction in the number of lymphocytes and neutrophils subsequently decreased, but only the second contact CY was observed immunosuppression. The analysis of the genotoxicity of cyclophosphamide (CY) was analyzed using the comet assay and was of paramount importance because dectamos genomic damage occurring in DNA exposed to different doses of cyclophosphamide (CY), which were 50 mg / kg in the first two phases and 25 mg / kg during the last two phases of the experiment. Furthermore, it was found that the genotoxic effects are cumulative with each CY dose applied, because even being administered in the third phase, the middle concentration (25 mg / kg) of the two inoculations initial CY the damage index does not correspond to half damage indices of the first and second vaccination. However, the analysis and immunologically genotoxicamente rodents, our work will enable testing new therapeutic immunosuppression regimens.
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Avaliação de microRNAs circulantes na esquizofrenia: da desregulação epigenômica a potenciais biomarcadores
(Universidade Federal do Pará, 2021-03) RODRIGUES, André Luiz de Souza; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099; https://orcid.org/0000-0002-4872-234X
Introduction: Schizophrenia is a serious and complex pathology that affects about 0.5-1% of the world's population. For the clinical diagnosis of schizophrenia, there are clinical criteria to be evaluated, which include both positive and negative symptoms. In the origin of the disease, there is a close relationship between environmental stimuli, and strong evidence shows that these stimuli have the ability to act on epigenetic mechanisms, which act in the regulation of gene expression. MicroRNAs (miRNAs) are stable and potentially reliable biomarkers, and some miRNAs have been previously identified as potential biomarkers for schizophrenia in peripheral samples. Objective: To evaluate the expression profile of circulating miRNA's in patients with schizophrenia (hsa-miR-34a, miR-449a, miR-564, miR-432, miR-548d, miR-572 and miR-652) in relation to control individuals negative for the disease. Methods: Analytical, case-control, cross- sectional study using samples previously collected from patients diagnosed with Schizophrenia (N = 650) and control group (N = 924), who adequately met the inclusion criteria. The samples were analyzed after RNA extraction through its quantification and techniques for obtaining reverse transcriptase reaction and real-time quantitative polymerase chain reaction. All data were analyzed using IBM SPSS22 statistical program. Results: Using the peripheral blood collection method with the intention of finding possible biomarkers for schizophrenia, an increased expression of the miRNA’s miR-34a, miR-449a, miR-564, miR-432, miR-548d, miR-572 and miR-652 was observed in several scenarios analyzed, confronting the case and control groups, as well as variables within the case-group, demonstrating potential diagnostic value.
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Avaliação seriada do perfil hematológico e bioquímico de primatas não humanos da espécie Sapajus apella tratados com lde-oleato de paclitaxel como instrumento para a terapêutica do câncer
(Universidade Federal do Pará, 2017-02-23) OLIVEIRA, Nayara Cristina Lima de; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Study of a chemotherapy delivery system, called LDE, with lipid composition similar to the natural low density lipoproteins of the body, denominated by the acronym LDL. LDEs have advantages over commercial chemical forms, since it is able to concentrate in the neoplastic tissues after injection into the circulatory chain, thus being able to target the tumors. LDE can be used as a "carrier" of paclitaxel (PTX) for possible reduction of toxicity and increase of its therapeutic action. The use of non-human primates as in vivo experimental models are of great importance in human health applications due to their anatomical, biochemical and phylogenetic similarities with human primates, generating results that can be interpreted more closely and safely to The phenomena in humans. The aim of the project was to evaluate the chronic toxicity of nanoparticles associated with to chemotherapy Paclitaxel (LDE-PTX) in individuals of the Sapajus apella species, based on the determination of hematological and biochemical parameters and their possible alterations. During the research 15 animals were used, divided into groups: Negative control (CN); Experimental (EXP1 and EXP2) where the animals received LDE-PTX intravenously two different doses of 175 mg / m2 and 250 mg / m2 respectively; and positive control (CP1 and CP2) where the animals intravenously received the drug in commercial form at the same doses used in the experimental group, respectively. Primates were accompanied for 6 cycles of chemotherapy, with interval of 3 weeks. Hematological and biochemical analysis was performed at each cycle through erythrogram and leukogram, alkaline phosphatase, total protein, albumin and globulin, total bilirubin and fractions, glycemia, amylase and serum lipase. E of the sodium and potassium eletrolytes were carried out in the serum of the animals during the collection days. Data were expressed as mean ± standard deviation and submitted to analysis of variance ANOVA, with Bonferroni post-test with significance for p <0.05, through BioEstat®5.3. The obtained results demonstrated advantages of the use of LDE-PTX, since the hematological tests demonstrate that there was a lower toxicity in all the chemotherapeutic cycles and the non-alteration of the majority of the biochemical parameters, demonstrate that the toxicity of the tested drug associated to LDE present smaller Effect toxic than its commercial version. It was concluded from the analysis of the results that hematological and biochemical toxicity was lower in treatment with PTX associated with LDE than treatment of PTX in its commercial form.
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Comparação cromossômica intrapopulacional da espécie Tayassu tajacu criada em cativeiro
(Universidade Federal do Pará, 2005-04-13) SOUZA, Patrícia Carvalho de; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
The collared peccary (Tayassu tajacu) is found from southern United States to northern Argentina. In Brazil, it ranges all over the country and is a source of meat for local people. Thus, a cytogenetic analysis is an important tool to evaluate reproductive efficiency at animals breeding in captivity that in order to commercialization. The aim of this study was to determine the chromosomal number of this specie from animals breeding in captivity of different populations. The animals utilized in this work were from different Brazilian populations: 6 animals from Mossoró (RN), 1 from Ipixuna (PA) and 4 from Uruará (PA). Metaphase chromosomes were prepared from cultured blood lymphocytes, following standard procedures. The results showed the same karyotypic pattern found for others authors (2n = 30 chromosomes and FN = 48), although, not any differences were found among these populations. But, they showed the same South American pattern, however, chromosomal polymorphism were found when compared to others populations, in Brazil and others countries (United States and French Guiana).
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Correlação entre medidas quantitativas de espessura retiniana, concentração de metabólitos encefálicos e funções neuropsicológicas de pacientes com transtornos neurocognitivos
(Universidade Federal do Pará, 2017-10-17) WAN-MEYL, Fabio da Silva; TEIXEIRA, Cláudio Eduardo Corrêa; http://lattes.cnpq.br/7448998858430931; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Currently, with the increase in life expectancy of human populations, neurodegenerative diseases, commonly occurring with the advancing age, have become a source of serious concern. Thus, research into new ways of early diagnosis of neurocognitive disorders associated with diseases such as Alzheimer's and Parkinson's, as well as the improvement of the understanding of currently available clinical neurological diagnostic methods, has been promoted throughout the world. In this sense, this work proposes to investigate the existence of possible correlations between the results of exams used in clinical neurological investigation of patients diagnosed with neurocognitive disorder associated with these important neurodegenerative diseases (Alzheimer's and Parkinson's diseases). The results show that the measurement of the thickness of the nerve fiber layer of the perimacular retina, performed by optical coherence tomography, is a parameter that may not differ significantly between groups of patients and healthy subjects. On the other hand, the measurement of the amplitude of the spectroscopic signals generated by encephalic metabolites, performed by magnetic resonance proton spectroscopy, reveals encephalic changes that vary from region to region. In addition, the neuropsychological measure of cognitive functions, performed by the automated CANTAB battery, reveals that several aspects of these functions are impaired in these patients. Finally, Principal Component Analysis shows that, considering the set of variables obtained by tomographic and neuropsychological measurements, it is possible to observe a correlation between several of these variables. Thus, it is concluded that correlating the results obtained by different approaches may add potential in the interpretation of this casuistry, which would not be possible if we consider such data in an isolated way.
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Estudo da influência do uso de agrotóxicos e de polimorfismo do gene GSTT1 na etiologia de fissuras labiopalatais em pacientes do Estado do Pará
(Universidade Federal do Pará, 2014-08-28) MARTINS, Cláudia Maria da Rocha; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Birth defect or congenital malformation is any anatomic, metabolic or functional abnormality, inherited by a mechanism of Mendelian transmission or caused by a new mutation, a chromosomal alteration or physical aggression by an infectious, chemical or the fetus or embryo development . Its causes may be genetic or environmental, and, most often, are multifactorial origin, through the genetic predisposition factors interact with environmental factors triggers. In Pará state, a large number of individuals affected by Oral clefts are from rural areas, mainly in the northeastern state where it is notoriously indiscriminate use of pesticides harmful to human health, many of which have high potential teratogenic. The objective of our study was to investigate the association between the polymorphism (rs4630) in the GSTT1 gene and exposure to pesticides in etiology of oral clefts and analyze the pattern of changes in speech of patients according to the type of cleft. Eight three patients with cleft palate or lip, and of both sexes, and 83 mothers of these patients, all from the state of Pará, residing in rural and capital area were analyzed. Speech therapy and analyzes were performed with the blood of these individuals, the molecular analysis was performed. Statistical analysis was performed using the statistical software SPSS v.. 12.0 and BioEstat v. 5.0. Tests included testing multiple logistic regression test x2e the Fisher exact test. Our result consists of five different molecular analyzes. We found that the presence of the C allele in the genotype of the individual can influence the metabolism of xenobiotics and increase the risk to develop oral clift.
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Expressão dos genes C-MYC, HER-2 e receptores hormonais como preditores de resposta à quimioterapia neoadjuvante em câncer mamário
(Universidade Federal do Pará, 2010-08-02) PEREIRA, Cynthia Mara Brito Lins; DEMACHKI, Samia; http://lattes.cnpq.br/7568391537270652; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Breast cancer is a higher incidence of tumors in women, and therefore many studies have been performed since the assessment of the epidemiological characteristics, the dynamics biocelular and treatment of this disease. In evaluating responses to treatment, the risk factors are markers that help in choosing the best drug to use. This work aimed to evaluate the genes of estrogen and progesterone receptors, HER-2 and C-MYC in locally advanced breast tumors as predictors of response to neoadjuvant chemotherapy. We studied fragments of mammary malignancy in 50 patients with infiltrating ductal carcinoma, with clinical stage III and E-treated with neoadjuvant chemotherapy. We used the techniques of immunohistochemistry and fluorescence in situ hybridization (FISH). The analysis of hormone receptors showed no statistically significant difference comparing patients with satisfactory response to chemotherapy, the poor and the analysis of HER-2 showed significance only for satisfactory answers, where there was poor amplification of this gene. Regarding C-MYC was observed a statistically significant difference comparing the high amplification of this gene to a poor response to chemotherapy. The study concluded that the C-MYC gene may be an important marker for predicting the treatments used in neoadjuvant chemotherapy for breast cancer.
Acesso aberto (Open Access)
Homocisteína vitamina B12 e ácido fólico como biomarcadores de triagem no diagnóstico precoce e monitoramento do câncer gástrico
(Universidade Federal do Pará, 2018-10-11) ALCÂNTARA, Fernanda Farias de; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Gastric cancer in the last decades has shown a decrease in the number of cases, which is much due to the progress in sanitary health, and the greater access of the population to educational policies. However, it remains the third leading cause of death worldwide between men and women. Such deaths are usually linked to late diagnosis. The present study intends to establish a profile of screening biomarkers by the homocysteine, vitamin B12 and folic acid dosages, which can be inserted in the routine routine of examinations, aiming the rapid diagnosis of the disease. A total of 207 control and 207 cases of gastric cancer patients were analyzed, both of which were biochemical measurements of homocysteine, folic acid and vitamin B12, matched in relation to age, tumor location, subtype, tumor classification, EBV (Epstein-Barr Virus), and Helicobacter pylori. For the triad dosage, chemiluminescence techniques were used, and the other variables were obtained by hospital information. As results, significant differences were found between the means of the triad of cancer patients in relation to the control, in all paired variables. In conclusion, our study showed that the triad analysis (homocysteine, vitamin b12 and folic acid) has its value in the diagnosis of gastric cancer, and may in the future be an effective marker of screening for this type of cancer.
Acesso aberto (Open Access)
Imunoexpressão da proteína PTEN em amostras de carcinoma epidermoide bucal e sua correlação com características clínico-patológicas e sobrevida
(Universidade Federal do Pará, 2016-06-03) KATO, Valdenira de Jesus Oliveira; PONTES, Hélder Antônio Rebelo; http://lattes.cnpq.br/8076555757131891; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Oral Squamous Cell Carcinoma (OSCC) is the most common malignant neoplastic that affects the oral cavity, accounting for 90% of all cases diagnosed in this anatomic site. Despite the recent advances in the treatment, the survival rate varies from 30 to 50%. Molecular mechanisms which elucidates the agressive behaviour of these lesions help to identify new chemotherapeutics that might be used in the treatment in order to improve the survival rates. The aim of this study was investigating the imunoexpression of PTEN protein through the immunohistochemical technique (IHC) in CEB samples and relate them with clinicopathological features, histological grades and survival and in addition, evaluate the presence of allelic deletion through the Fluorescent In Situ Hybridization technique (FISH). Our results showed that a total from 119 cases of CEB, 31 cases were negative to expression of protein PTEN and 88 cases were positive for PTEN, from which 15 (17,05%) were well differentiated, 43 (48,86%) moderately differentiated and 30 (34,09%) were poorly differentiated. Considering the clinicophatological features, there were not statistically significant correlations with the IHC expression of PTEN. Regarding the survival rates, it was observed that patients presenting lymph node infiltration (N) = 2 or 3 has 4 times greater risk of dying than those presenting N = 0 or 1. Finally, there was a significant association between expression by IHC negative and the result of FISH technique regarding the deletion, and between the positive PTEN expression and the non-deleted result of FISH technique.
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Prevalência de Helicobacter pylori e vírus Epstein-barr em crianças e adolescentes
(Universidade Federal do Pará, 2013-03-13) OLIVEIRA, Kátia Soares de; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Introduction: Infections by Helicobacter pylori (HP) and Epstein-Barr virus (EBV) are common worldwide, although HP is the highest factor in gastroduodenal diseases, its percentage of association with EBV is uncertain. Both EBV and HP are classified are class 1 carcinogens by the World Health Organization, and a substantial number of individuals become co-infected in adulthood. These two pathogens may have synergic potential to cause perpetual chronic gastritis. The purpose of this paper was to verify the prevalence of HP and Epstein-Barr virus in children and adolescents. Material and Methods: Transversal descriptive study. The gastric mucosa of 64 children and adolescents was analyzed through the Urease Test to diagnose HP and the PCR technique to detect H. pylori’s cagA strain, the in situ hybridization technique to detect EBV and the pathological analysis to determine the histopathological characteristics. Results: The prevalence of HP and EBV found by this study was 53.1% and 3.1, respectively. Most of the patients infected by HP (94.3%) presented gastritis in the upper gastrointestinal endoscopy, with enanthemathous gastritis being the most commonly found type. In the histopathological analysis, most patients (97.1%) presented some level of gastritis, 80% of which classified as moderate chronic gastritis. Positive cagA strains were found in 64.7% of the patients infected with HP and all of them had gastritis, with predominance of moderate chronic gastritis (54%); however, there was no statistically significant correlation between these findings. There was also no statistically significant association between infection by HP and EBV in the studied population. The low prevalence of EBV in this analysis suggests that this virus is not an etiological agent in gastric mucosa lesions. To our knowledge, this is the first study that relates these two infectious agents in the gastric mucosa of children and adolescents in northern Brazil. Conclusion: Most of the findings in this study are in line with the literature; however, it is necessary to conduct larger studies, involving aimmunocompetent pediatric population in order to determine whether there is a correlation between infection by HP and EBV in our region.
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Prevalência do gene TSPY em pacientes com anomalias do desenvolvimento sexual no Pará
(Universidade Federal do Pará, 2014-04-14) GARCIA, Lena Stilianidi; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Patients with disorders of sexual differentiation (DDS) present higher risk of neoplasies. The most common neoplastic changes in these patients are: the gonadoblastoma, carcinomain situ and cell germ tumors of intra-tubular unclassified. The type II germ cells are precursors these lesions in most cases. The gonadoblastoma is a benign tumor that no metastasizes, but the high prevalence and risk of progression to malignant forms of gonadal neoplasms, deserves special attention. In a close to the centromere on the short arm of the Y chromosome region, the TSPY gene was isolated, counted as the gonadoblastoma gene. Expressed in large amounts in cells that constitute the gonadoblastoma. DDS 47 patients were evaluated in their karyotypes and research investigated the prevalence of TSPY PCR. The analysis reveled that 50% of patients with Turner syndrome, even without the Y chromosome, righteous or not, evident in the karyotype, were positive for the presence of the TSPY gene. Evidencing the importance of the gene in the monitoring and guidance of gonadectomy in patients with DDS.
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Prevalência e associação da infecção gástrica por Helicobacter pylori e do vírus Epstein-Barr em casos de gastrite na população do Amapá
(Universidade Federal do Pará, 2017-11-03) ALVES, Nélisson Clei Ferreira; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
The epidemiology of HP and of the Epstein-Barr virus (EBV) is worldwide. The prevalence of both carcinogenic agents, in the world human population is about 45%. A recent study suggests that EBV coinfection with HP cagA positive increases the oncogenic potential of this bacterium. The objective of this study was to identify the prevalence of the bacterium HP and of the virus EBV and the association of those pathogens and of the cagA gene in patients with gastritis in the population of Amapá. A descriptive study was accomplished, of the traversal type, where 292 samples of gastric mucous of the patients were analyzed with gastritis submitted to the endoscopy, age group between 14 and 83 years. For detection of HP, Urease test and Polymerase Chain reaction were used; this methodology was also useful to reveal the positive cagA of the bacterium. Additionally, the technique of in situ hybridization was used for detection of EBV and the microscopic analysis that determined the histopathological characteristics of the gastric mucous. Results: The study showed high prevalence of cases of HP in patients with gastritis with a relative frequency of 87,67% of the 292 analyzed cases, a higher incidence, of HP positive cases, in female, 88,27%. The incidence of the cagA gene in samples of positive patients for HP was 72,66%, higher prevalence in female, 75,32%. In the present study 8,59% of the patients were found with viral infection caused by EBV in positive samples for HP with bigger prevalence in male, 9,18%. According to the age group, the study showed higher prevalence of the gene cagA and of EBV in positive patient for HP in the age group between 44 and 54 years, with 23,12% and 36,37%, respectively. Conclusion: Most of the findings of this study are similar to the reports from the literature, however, it is necessary other studies in order to explain if there is or there is no correlation between the infection for HP and EBV in the north of Brazil.
Acesso aberto (Open Access)
Valor prognóstico do SNP TP53 ARG72PRO na susceptibilidade ao desenvolvimento e na sobrevida de pacientes brasileiros com meduloblastoma
(Universidade Federal do Pará, 2011-12-22) CARVALHO, Raimundo Miranda de; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Medulloblastoma is a highly cellular malignant embryonal neoplasm, and it is the most common malignant pediatric brain tumor, comprising 20 to 25% of pediatric central nervous systen tumors. To investigate the role of the TP53 Arg72Pro single nucleotide polymorphism (SNP) on medulloblastoma risk, medulloblastoma prognosis, and adjuvant therapy response, we performed a case-control study with 122 patientes and 122 healthy controls from Brazil. Compared to Arg/Arg, which is the most common genotype in the study population, both the Arg/Pro and Pro/Pro genotypes did not influence the medulloblastoma development risk (OR= 1.36 and P= 0.339 for the Arg/Pro genotype; OR=1.50 and P=0.389 for the Pro/Pro genotype). With regard to prognosis, the disease-free survival was not significantly different among the TP53 Arg72Pro SNP genotypes (P > 0.05), but the less frequent genotype, Pro/Pro, was associated with a shorter overall survival of medulloblastoma patients (P= 0.021). These data suggested that although there is no association between the TP53 Arg72Pro and medulloblastoma risk, the Pro/Pro genotype is associated with a shorter overall survival of patients submitted to adjuvant therapy. Nevertheless, due to the interethnic composition of the Brazilian population, future studies on larger populations from other parts of the world are essential for a definitive conclusion of the function of the TP53 Arg72Pro SNP

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