Navegando por Orientadores "CORVELO, Tereza Cristina de Oliveira"

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Avaliação do estado secretor ABH e Lewis em mulheres não grávidas com e sem risco de desenvolver vulvovaginite por Candida sp
(Universidade Federal do Pará, 2003) CARNEIRO, Ligia Maia; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
Na epidemiologic study of Candida sp infection prevalence in non-pregnant women was performed in a population of the north of Brazil (Belem-Pa, 2002). This paper aims to contribute to clear up the infection mechanisms of Candida sp and its possible association to ABO and Lewis group blood antigens and ABH substances secretory status. Such paper comprehended a total of 165 women admitted at the out-patient clinic of "Hospital da Policia Militar" and "Laboratorio de Analises Clinicas do Centro de Ciencias Biologicas da Universidade Federal do Para", who came to those units to undergo gynaecologic examinations, where blood, salive and vaginal mucus samples were taken. Candida sp infection presence was determined through the examination of fresh vaginal secretion and bacterioscopy of vaginal secretion. The identification of ABH and Lewis phenotipes in the blood was determined through direct hemaglutination tests and, in the salive, by Elisa Dot-blot. It was performed a standard form for the obtaining of epidemiologic information. Candida sp prevalation was 47.9%. Among the most commom signals and symptoms associed to Candida sp infections are pruritus and gleet. Women under 40 who did not use condoms and who have already had previous infections presented a higher risk of vulvo-vaginal infection caused by Candida sp. Infected and non-infected women with Candida sp had similar distribuition to the phenotypes of ABO, Lewis blood groups and ABH substances secretory status. And, comparing the expressions of Lewis antigens in the blood, save and vaginal mucus, it was verified that both secretions expressed Lewis antigens without any close relationship to erithrocitic Lewis phenotypes. In such context, it is considered that the nature of genetics diversity in the interaction between Candida sp and host requires more studies involving the identification of different types to determine their probable associations among ABH and Lewis blood groups phenotypes with cadidiasis.
Acesso aberto (Open Access)
Características epidemiológicas relativas à doença dental e infecção por Helicobacter pylori na cavidade oral de estudantes em Belém-Pará
(Universidade Federal do Pará, 2009) MATOS, Gyselly de Cássia Bastos de; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
The infection by Helicobacter pylori is one of the most common in humans and despite having tropism by stomach, can be found in the oral cavity, maintaining a commensal relationship with the host, while dental caries is also an infectious disease and results from the metabolism of the bacterial plaque. Both infections are highly prevalent in developing countries, since these populations are more exposed to environmental risk factors, and are usually acquired during childhood. The prevalence of these infections was investigated in the oral cavity of school children with no symptoms of gastric diseases, from a population of Belém-Pa, in relation to some parameters of oral hygiene and health, socioeconomic conditions and genetic susceptibility factors like the ABO and Lewis blood groups. Were investigated 104 patients, with average age of 17 years. Of all the participants were collected saliva samples and dental plaque. Saliva was collected to identify the ABO and Lewis state secretor and estimation of salivary parameters, and both, saliva and plaque samples were collected for molecular analysis of 16S rRNA genes of H. pylori and FUT2. H. pylori was detected in 79.8% of the students, with a frequency of 66.35% in dental plaque and 58.65% in saliva. The caries prevalence was 82.8% in the population studied. The clinical evaluation of oral health showed that the average CPO-D found was 3.53. It was observed that the caries experience tends to increase as in addition to age and the H. pylori infection was higher in early childhood. The education level and number of dentist visits showed significant differences in relation to the presence of H. pylori. The phenotypic distribution of ABO and Lewis blood groups did not differ significantly between infected and uninfected individuals, explaining there is greater genetic susceptibility to infection by H. pylori and dental caries. Throughout this analysis, the high frequencies found prove the need for care and treatment of dental diseases, such as caries and it is suggested that H. pylori in the oral cavity can contribute to infection and re-infection of the stomach after treatment.
Acesso aberto (Open Access)
Comparação das cepas de Helicobacter pylori na placa bacteriana dental e mucosa gástrica
(Universidade Federal do Pará, 2006) ASSUMPÇÃO, Mônica Baraúna de; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
Helicobacter pylori infection is extremely frequent over the world, mainly in development countries, including Brazil. It’s associated to chronic gastritis, duodenal and gastric ulcer, and is considered as an important risk factor for gastric cancer and gastric MALT lymphoma. The transmission routes remain unclear, but oral-oral and fecal-oral routs seem to be the most probable ones. The value of the presence of the bacteria on the dental plaque also remains unclear, and it maybe a source for gastric infection. Aiming in identifying and correlating the H. pylori stains found in gastric mucosa and dental plaque of 99 adult dyspeptic patients submitted to upper digestive endoscopies at Hospital Universitário João de Barros Barreto, in 2005 were evaluated. Samples from dental plaque were collected by sterile sticks and urease test and polymerase (PCR) chain reaction were undertaken. During the endoscopic procedure 6 pieces were collected from antrun and investigated by urease test, histopathology and PCR, after obtaining informed consent. The results were analyzed using BioEstat 3.0 package. The bacteria was found in 96% of gastric samples and in 72% of dental plaque samples, and this difference was statistically significant (p<0,001). There weren’t statistically significant differences related to age or gender. Every patient presented gastric diseases. In 18% of the cases lessons considered as of higher severity such as ulcers or pre-malignant lesions, as intestinal metaplasia, were found, and, among these, there were 82.4% of cases with both gastric and dental plaque infection. PCR was the most efficient test either on dental plaque and gastric mucosa samples. Among the 71 cases where the dental plaque samples were positive for the presence of the bacteria, the stains were identical to the gastric mucosa H pylori stains in 89%. The most common genotype was s1bm1cagA positive, either at dental plaque and gastric mucosa. The type 1 strains, considered the most pathogenic ones, were found in 63 patients on gastric mucosa and in 58 patients on dental plaque. The high frequency of H. pylori found on dental plaque might indicate the oral cavity as a colonizing locus for this bacteria and a risk factor for gastric infection.
Acesso aberto (Open Access)
Diversidade genética das espécies de micobactérias não tuberculosas identificadas em laboratório de referência para o diagnóstico da tuberculose na região Norte
(Universidade Federal do Pará, 2009-02-20) COSTA, Ana Roberta Fusco da; LIMA, Karla Valéria Batista; http://lattes.cnpq.br/9795461154139260; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
Recent years has seen an increase in reports of infections associated with nontuberculous mycobacteria (NTM). However, knowledge of the frequency and the species involved in pulmonary and non-pulmonary infections in Brazil are limited. We evaluated the occurrence of species of NTM isolated Mycobacteria Laboratory of the Instituto Evandro Chagas, Regional Laboratory of Public Health of the North. We analyzed all NTM isolates from pulmonary clinical specimens of individuals with infection, according to the criteria of the American Thoracic Society and the Department of Health between the years 2004 to 2007. The NTM were characterized by PCR-restriction analysis (PRA-hsp65) and 16S rRNA, hsp65 e rpoB genes sequencing. We identified 51 patients with infection caused by NTM, which the M. abscessus (n=2), M. bolletii (n=4), M. massiliense (n=9), M. avium (n=5), M. colombiense (n=5), M. fortuitum (n=4), M. simiae (n=2), M. interjectum (n=4), M. intracellulare (n=5), M. kansasii (n=1), M. scrofulaceum (n=1) e M. terrae (n=1) species that involved in lung infection. In eight individuals the NTM isolates were not identified at the species level, which may represent new taxonomic entity from M. simiae complex. This study describes the diversity of NTM isolated from pulmonary clinical specimens in the state of Pará, Brazilian Amazon Region. The finding cases of lung infections diagnosed and treated unsuccessfully for several months as tuberculosis suggest the need for isolation and identification of mycobacteria involved before establishment of treatment failure.
Acesso aberto (Open Access)
Febre tifóide: a experiência do Instituto Evandro Chagas
(Universidade Federal do Pará, 2005) RAMOS, Francisco Lúzio de Paula; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
Typhoid fever is an infection disease of world occurrence, but as it is strictly related with low socioeconomic levels it occurs most frequently in developing countries. In this work we present the experience of Instituto Evandro Chagas (IEC) with that disease since 1987 up to 2004, period in which a number of 443 cases was accumulated, all diagnosed by Salmonella Typhi’s isolation from blood or feces, being some of them complemented with Widal serologic reaction. The case were proceeding from Belém and the municipal districts of Pará state conduced to IEC by the respective Units from Health System for etiologic diagnosis of fever syndrome, most of them of persistent course, here designated as “spontaneous” demand, at that time detected during outbreak investigations occurred in the municipal districts. We discussed aspects related to the clinical presentation, with emphasis to the atypical manifestations, the distribution according to sex and age group, the seasoning, the distribution by origin area, identifying the municipal districts of larger prevalence, and the distribution by districts, in relation to the cases coming from Belém. We also discussed aspects related to the laboratorial diagnosis emphasizing the application of the cultivation methods (coproculture and hemoculture) comparing their performance concerning the time of disease and the relationship of those tests with Widal reaction, by measuring the value of these as auxiliary or complementary method in the diagnosis of the disease. Clinical, epidemiological and laboratorial analyses were made, using Bio Estat version 3.0 program by applying qui-square and G test and the statistic significance was accepted at 95% level. The results allowed us to have the following conclusions: 1) in situations of epidemic normality the typhoid fever affects more the young adult group, while, in the epidemic situations, the affected age group is the infantile; 2) there is homogeneity among the samples from cases of outbreaks and the spontaneous demand according to the gender distribution, but males are more exposed to typhoid fever; 3) in municipal districts of the State the disease was shown to be more frequent in the areas where it concentrates larger number of populations who live near rivers and, in relation to the capital, it showed larger prevalence in jurunas district; 4) the illness has a seasonal profile, which shows the largest occurrence in the second half of the year, probably favored by geo-climatic and cultural factors; 5) the typhoid fever constitutes serious problem of public health en the State do Pará, confirming its narrow relationship with the high poverty levels; 6) the leukocytic formula revealed normal pattern or leucocytosis compared to leucopenia; 7) in the laboratorial approach it should not dispense the assays that seek to the isolation in detriment of the serological test, and the coproculture and the hemoculture should be requested in all the cases without considering the time of evolution of the clinical situation; 8) in our area, where there is not availability of cultivation tests, Widal reaction can be an alternative of value diagnosis; 9) it is illness of persistent clinical course and with atypical clinical manifestations (pneumonias and cholestatic hepatitis) whose sings/symptoms, when presented, should raise the suspicion by the investigator; 10) in the areas high endemicity, the typhoid fever can cause a negative impact in the economy not only for the costs generated with laboratorial exams, treatment, hospitalizations and eventual surgical interventions, but also because it can let the worker to be away from work for a prolonged period of time.
Acesso aberto (Open Access)
Infecção por Helicobacter pylori: transmissão intradomiciliar e os fenótipos de grupos sanguíneos ABO e Lewis como marcadores de predisposição entre as famílias residentes as margens do Rio Tocantins, no município de Imperatriz – MA
(Universidade Federal do Pará, 2012-09-13) BARBOSA, Marluce Sampaio Nobre; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
The present study aimed to analyze the relationship between infection by Helicobacter pylori in children and their parents through diagnostic laboratory and epidemiological information, helping to clarify the possible etiological factors of this infection. A descriptive and analytical cross-sectional was conducted, from March to June 2012. The study population included 48 families living on the river in areas of the river Tocantins, in the municipal of Imperatriz-Maranhão, registered and assisted by the family health team operating in that area. Form epidemiological applied and biological material collected from children under 12 years corresponded to samples of feces and saliva, while the parents or guardians and children from 12 years corresponded to samples of blood and saliva. Serum samples were screened for the presence of anti-H. pylori by immunoassays (ELISA) was used in saliva DOT-ELISA technique on nitrocellulose membranes for identifying phenotypes ABH and Lewis, feces were used for the detection of H. pylori antigens using immune chromatographic assay qualitatively. The overall prevalence of infection in children under 12 years was 69,23%, with onset before the first year of life. The prevalence of infection in mothers and fathers was 76.60% and 59.09% respectively; between infected mothers 77,27% of the children were also infected. The prevalence of infection by H. pylori, among the members of the families studied showed no associations with the phenotypes of blood groups ABO, Lewis and secretor status. Socioeconomic aspects are suggestive of interfamilial transmission that can be facilitated by poor environmental conditions, with lack of sanitation, hygiene and poverty.
Acesso aberto (Open Access)
Marcadores imunológicos na exposição mercurial na Região Amazônica
(Universidade Federal do Pará, 2007) ALMEIDA, Sintia Silva de; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
Immune dysfunctions can appear by combination between genetic susceptibility and environmental factors. There are evidences, in humans exposed to total mercury (Hgt), of alterations in immune response, by Hgt induced autoantibodies. This work investigated the occurrence of Hgt-induced autoimmunity (HgtIA) among human being exposed to Hgt in the Tapajós region (Barreiras, Brasília Legal, São Luís do Tapajós), as well as in the riverines from Tocantins region (Panacauera) without exposition to Hgt. The of hair and blood samples were collected from 236 individuals during June of 2004 to December of 2006, in which the total mercury in hair was determined by atomic absorption spectrophotometer, and in the serum, autoantibodies were analyzed by indirect immunofluorescence microscopy, using human epithelial cells (HEp-2) as substrate. The highest Hgtotal levels was found in São Luís do Tapajós (11.24 ± 2.23 μg/g), following for Brasília Legal (10.00 ± 0.99) and Barreiras (8.64 ± 1.13), and the lowest were found in Panacauera (2.98 ± 0.20). When considering the variable sex, association with the levels of Hgtotal in hair was observed only in Brasília Legal. Regarding mercury exposure, the autoantibodies in serum was more frequent in riverine of the Tapajós (79.65%) than in the control group (31.25%). Most of the autoantibodies patterns identified by immunofluorescence were: mixed (50.96%), nuclear (31.21%), nucleolar (14.65%) and mitotic apparatus/cytoplasmatic (3.18%). The mixed and nuclear autoantibodies patterns were observed in exposed communities (p<0.01). In addition, various widely recognized autoimmune disease specific autoantibodies were also found, which occurred more frequently as following: NuMa1, PM/Scl, Ssa-Ro, rRNP/Sm, golgi/Ssa/Ro, PCNA, rRNP, Ku, besides other autoantibodies without defined specificity. The intensity of immunofluorescence was more reactive in riverine of the Tapajós (p<0.0001). Analysis by regression logistics multiple indicated that the risk of autoantibodies present in serum was twice higher in riverines exposed to mercury (>10μg/g) with age group above 50 years (p>0.01). Finally, additional studies are indispensable to confirm the specificity of these autoantibodies associated with the mercurial exposure, as well as to elucidate the mechanisms immunotoxicologicals of action of the mercury on the human immune system.
Acesso aberto (Open Access)
Parasitismo intestinal: estudo epidemiológico em dois grupos sócio-econômicos distintos de uma população infantil de Porto Velho-RO
(Universidade Federal do Pará, 2003-12-15) AQUINO, Eulália Gonçalves de; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
Parasitic infections are present in endemic ways in several areas of Brazil. The investigation estimated the prevalence of enteroparasites in 200 children between 2 and 13 years belonging to two distinct social-economic classes, middle and low, in a period from April to June in 2001 in the city of Porto Velho, RO. And it also analyzed the contextual risk factors of greater epidemiologic importance. The samples of the feces were examined through direct and spontaneous sedimentation method. The population of middle social-economic level (A) made up of 100 individuals, presented a positivity rate of 18 %, being the most prevalent parasite Giardia lamblia (52,4 %), followed by Ascaris lumbricoids (19 %), Trichuris trichiura (14,3%), Enterobius vermicularis (4,8%), Hymenolepis nana (4,8%) and Endolimax nana (4,8%). In the group of low social-economic level (B) the positivity reached 56 % of the individuals also being Giardia lamblia (28,9%) the most prevalent, followed by Entamoeba coli (22,7%), A. lumbricoides (14,4%), T. Trichiura (8,3%), E. vermicularis (7,2%), H. nana (4,1%), E. nana (4,1%), E. histolytica (4,1%), Ancylostomídeos (3,1%) and leaven (3,1%). The occurrence of more than one parasite per individual was more prevalent in among the children of group B (46,4%), being that the parasitism was more prevalent among children with gastrointestinal symptoms in both groups A and B. The intestinal parasitosis affected boys and girls equally; Negroid and Caucasian and occurred equally in both two age group of children under and above five years, however, children under the age of five of group A presented preponderance of infection by protozoan of the specie G. lamblia. Low income and houses with a great number of people cohabiting and without water supply net constituted social-economic risk factors that support the high prevalence intestinal parasitosis. Anemia and nutritional state conditions were also evaluated and did not show significant relations with enteroparasitosis, in the different social-economic groups. Therefore, high prevalence in children, especially of low social-economic level, with intestinal parasitism, anemia and moderate acute malnutrition, reflect the precarious public healthy conditions in the city of Porto Velho, RO.
Acesso aberto (Open Access)
Prevalência e aspectos epidemiológicos das enteroparasitoses e sua relação com o estado nutricional em crianças residentes no Bairro Beira Rio de Imperatriz, MA em 2011
(Universidade Federal do Pará, 2012) PIRES, Renata de Cássia Coêlho; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
Intestinal parasites are considered a public health problem, that despite all the advances in technology and medicine, are still strongly incidents today, being able to trigger different consequences to their patients, especially in children, who are in growth phase and development and may have harmed this time delay through the physical, mental and social. Thus, this research aims to understand the prevalence and epidemiology of intestinal parasites and their relation to nutritional status in children living in the neighborhood Beira-Rio, aged 01-10 years, who are accompanied by the Family Health Strategy of that district. Therefore, we analyzed 102 records concerning children attending the Basic Health Unit (BHU), through the care offered by the health team in the period between 2011 and underwent stool testing, hemoglobin and serum iron, beyond measure anthropometric. A prevalence of 60% for intestinal parasites, particularly Ascaris lumbricoides (20%), Giardia lamblia (14%) and Endolimax nana (14%). Regarding sex was not identified significant differences in the prevalence, however, was observed an association between intestinal parasites and serum iron anemia, unlike the analysis of hemoglobin. Nutritional assessment showed that (50.98%) of the children were with adequate nutritional status (eutrophic) and (34.32%) had abnormal underweight and nutritional risk, and of these (57.14%) were parasitized, the alerting for greater attention to this audience because of diseases and complications that may result from this condition. In epidemiology there was a significant relationship between low education and intestinal parasites, as well as disability treatment of water consumed, which reinforces that preventive measures are essential to control the condition, as well as the damage to health, reflect conditions of life to which the community is exposed as inadequate sanitation and poor quality of life, especially for children.
Acesso aberto (Open Access)
Soroepidemiologia da Helicobacter pylori em crianças e suas mães: avaliação dos fatores de risco
(Universidade Federal do Pará, 2003-04-29) CARTAGENES, Vivian Lúcia Aslan D' Annibale; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
An investigation with the objective of studying seroprevalence of infection by the bacterium Helicobacter pylori was carried out with 100 children, between 1 and 12 years of age at the João de Barros Barreto University Hospital in Belém, Brazil, and with their respective mothers. Possible risk factors related to infection were analysed and possible associations of infection between mothers and their children, including the CagA strains. Blood and saliva samples were collected from the participants and stool samples from the children. Anti-H. pylori serology was done using the indirect haemoagglutination method and anti-CagA was done by Elisa. The ABH and Lewis phenotypes in blood were determined with the direct haemoagglutination test and in saliva by Elisa dot-blot. Antigens of the bacteria were studied in 79 stool samples from the children by Elisa capture. Personal and family information was obtained using a standard questionnaire. Seroprevalence among children was 50.0% and 86.0% among mothers. Seroprevalence among children increased with age (p < 0.05) and the habit of attending schools or creches (p < 0.05). The diagnostic methods Elisa capture and indirect haemoagglutination showed similar performance in children, with greater discordance observed in the 1 to 4 year age group (p < 0.05). Infected mothers represented a risk factor for infection in their children (p < 0.05), above all mothers with CagA strains (p < 0.05). The fact of coming from municipalities with 100 thousand or more inhabitants (p < 0.05), piped water (p < 0.05), absence of sanitary installations (p < 0.05) and sanitation in homes (p < 0.05) represented a risk for family infection. Transmission of H. pylori was facilitated by precarious hygiene and sanitation conditions, urban conglomerations and by human contact between children and their mothers, through faecal-oral, oral-oral and/or gastro-oral routes.
Acesso aberto (Open Access)
Soroepidemiologia da sífilis em gestantes e seus recém-nascidos
(Universidade Federal do Pará, 2003) RASSY, Maria Elizabete de Castro; CORVELO, Tereza Cristina de Oliveira; http://lattes.cnpq.br/7253864056606024
Syphilis is a systemic infectious-contagious disease caused by Treponema pallidium, a parasite that is exclusive to humans and whose transmission is essentially carried out through sexual contact. The incidence of congenital syphilis has reached truly epidemic proportions, although measures that could control this disease are known. The investigation described pregnant women with syphilis hospitalised at the FSCMPA during the period of January 2001 to June 2003, as to socio-demographic characteristics, behaviour for promoting sexual and reproductive health and serological and clinical data related to syphilis in the mother-newborn relationship. The study is of a crosscutting, analytical and descriptive nature, with 245 pregnant women. Medical records of these patients were reviewed, with positive serology for syphilis and the newbom, tested by the VDRL method. The incidence of syphilis in pregnant women was 1.73% of the total of patients hospitalised in the last two and a half years. The epidemiological profile demonstrated that the main risk factors for acquiring syphilis during pregnancy were: absence of prenatal accompaniment and treatment, including for the sexual partner. According to postpartum maternal seropositivity, it was found that the possibility of a newborn having syphilis, if VDRL positive, was 98.68%. Jaundice and low weight were the most common clinical signs of syphilis, and prematurity was also a clinical sign observed. These analyses reveal the need for educational campaigns of a broader scope related to risk factors for acquiring this infirmity and better care for pregnant women during the prenatal period, with accompaniment and diagnostic control for the pregnant women.