Navegando por Orientadores "SILVA FILHO, Manoel da"

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Análise de mutações no gene GJB2 em indivíduos com deficiência auditiva neurossensorial não sindrômica
(Universidade Federal do Pará, 2012-11) PAULA, Danilo Monteiro de; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Deafness is the most frequent sensorial defect in human beings and it may have different causes since environmental to hereditary. In developed countries the estimates suggest that in each 1000 births some kind of deafness is expressed and more than 60% of the cases have a genetic origin. In Brazil, the hereditary deafness is not well-known. It is believed that four in each thousand newborns express some kind of hearing defect and that the frequency of deafness caused by genetic factors is estimated in 16%, while the 84% remaining cases are caused by environmental factors and have an unknown etiology. The many forms of hereditary deafness already identified are very rare, except for the one which is caused by mutations in the GJB2 gene which codifies the connexin 26. The connexins represent a class of a protein family which is responsible for the formation of communications channels between adjacent cells (Gap Junctions), this communication is fundamental for the growth and differentiation of the tissues. Until now there have been described 102 mutations of GJB2 gene which are associated to the hereditary deafness. Three mutations stand out because they have high frequency in specific population groups: 35delG among Europeans and Brazilians, 167delT among Ashkenazi Jews, and 235delG among Asians. In this study, we performed a molecular analysis of the entire coding sequence of the GJB2 gene (Connexin 26) in a population sample consisted of 30 unrelated individuals with prelingual nonsyndromic sporadic deafness from the population of Belém do Pará. DNA was obtained by peripheral blood samples and analyzed by the conventional PCR followed by automatic sequencing. Mutations in the Connexin 26 gene were found in 20% of the sample (6/30). The mutations 35delG and R143W were observed in one patient (1/30), both in the heterozygous and related to the patient’s deafness. Two additional mutations were observed in different individuals: G160S in a patient corresponding to 3.3% (1/30), and V27I was observed in 4 patients with allele frequency of 0.08; however mutations G160S and V27I are not related deafness. In this work the observed frequencies of mutations are equivalent to the frequencies observed in other populations previously studied. These results indicate that mutations in the GJB2 gene are important causes of deafness in our region and it cannot be excluded that the possibility of deafness presented by some individuals may be caused, mainly, by environmental factors such as infectious processes occurring during pregnancy or the first months of life.
Acesso aberto (Open Access)
Aspectos morfológicos comparativos entre neurônios da camada I do córtex visual de duas espécies de roedores: Cavia porcellus e Rattus norvegicus
(Universidade Federal do Pará, 2014-08-20) MOREIRA, Thayana de Nazaré Araújo; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
The layer I has as main characteristic the low number of neurons and a high density of nerve fibers. The morphology of neurons of layer I is still understudied, so that in studies evaluating the morphology of these neurons has not yet reached a consensus on the forms and functions of these neurons. This study evaluated the morphology of neurons in layer I of the visual cortex of two rodent species: Cavia porcellus, popularly known in Brazil as a guinea pig and Rattus norvegicus, which is the rat and the Wistar strain was used, commonly used in scientific research. The guinea pig is a widely studied animal model used in several areas of science. Although this species is well studied, works on layer I of this animal are relatively rare, especially in relation to morphology and electrophysiology of neurons in this cortical region. Research in rats on neurons of layer I are more frequent, both in relation to morphology and electrophysiology. To discriminate the potential for differences in the morphology of neurons in layer I of the visual cortex of the guinea pig and mouse, this study classified these neurons according to the trajectory of their dendrites and dendritic measures analyzed using the technique of intracellular injection of biocytin. After classification of neurons comparisons were made between the same cell types of each rodent. 35 guinea India Dunkin-Hartley variety of short-haired of both sexes aged 4-5 days of postnatal life were used. As for the rats, 30 rats of Wistar variety of both sexes aged 14 to 21 days of postnatal life were used. The animals were anesthetized and had their brains removed, separated hemispheres and sections were made in the coronal plane in the occipital region where is located the visual area of rodents. Slices were maintained in artificial cerebrospinal fluid and then brought to the microscope to inject biocytin and subsequently were fixed and treated for mounting on slides and counterstained with Nissl for better viewing. Neurons found were classified as horizontal, ascending, descending and radial. The receptive field area, total and average dendritic length, total area of the cell body, dendrites number, distance from the pia mater and distribution analysis Sholl: The following dendritic measures were analyzed. Results of the most notable were the extent of dendritic branches and the size of the cell body of neurons of layer I of the guinea pig compared to rat. This suggests that in this species, a larger number of neural microcircuits can be established, and therefore greater metabolic rate justified by the size of the cell body.
Acesso aberto (Open Access)
Avaliação da marcha e do equilíbrio em pacientes portadores de síndrome lipodistrófica secundária à terapia antirretroviral
(Universidade Federal do Pará, 2013-09-25) LIMA, Ramon Costa de; CALLEGARI, Bianca; http://lattes.cnpq.br/0881363487176703; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
The Acquired Immune Deficiency Syndrome (AIDS ) is a disease that devastates the world's population decades and hear this diagnosis was like a " death sentence " . With the advent of new drug therapies, characteristic of the acute disease has become a chronic condition . However , the drugs used in antiretroviral therapy (ART ) have adverse reactions , especially when the patient is subjected to long-term use of so-called "cocktail " . One of the side effects of ART is lipodystrophy , which causes the molecular scale adpócitos apoptosis and mitochondrial alterations in the muscle fibers . Thus, the objective of this study was to investigate the effects of muscle Lipodystrophy and changing patterns of gait and balance of patients in this clinical contexto. Were evaluated 38 subjects of both sexes, divided into two groups: HIV positive with lipodystrophy (HIVL) and HIV positive without lipodystrophy (HIV). The balance test was used a force platform (EMGSystem of Brazil), which evaluates the displacement of the pressure Center (Cop) in the anteroposterior directions (AP) and mediolateral (ML) of the individual generating the variables the total linear displacement, total area of displacement and displacement velocity amplitude of the displacement at a time of sixty seconds for each collection. For the gait test we used the 8-channel Electromyograph (EMGSystem of Brazil) to capture the electrical signals of the muscles Rectus Femoral (RF), biceps femoral (BF), gastrocnemius Lateral (GL), anterior tibialis (TA) and Gluteus Médius (GMD) during ambulation and processing of electromyographic signal was made through the mathematical model Root Mean Square (RMS), and normalized by maximum voluntary contraction (MVC). The results of each group were expressed as mean and standard deviation and compared using the Student t test for parametric samples and the Mann-Whitney test for nonparametric samples. Analysis of the results in the two phases of the gait cycle showed significant differences. In the phase of support and swing phase electromyographic signals of GMD and TA muscle were higher in HIVL group for HIV group. As for the balance variables with statistical significance when comparing the groups were the total displacement and displacement area, both higher in HIVL group for HIV group. Thus we conclude that patients with lipodystrophy syndrome showed changing patterns of gait and balance.
Acesso aberto (Open Access)
Avaliação do método de fotooxidação do DiI por diodo emissor de luz (led): aspectos morfológicos de células horizontais da retina humana
(Universidade Federal do Pará, 2013-11-25) FARIAS, Flávia Moura Gaia; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
The analysis of cell morphology is a crucial aspect of neurobiology, since the relationship between form and function can define the physiological processes in health and disease. One of the main methods to evaluate cell morphology is through photooxidation of intracellular fluorescent markers, among which is the perchlorate of 1,1 '-dioctadecyl-3, 3,3', 3'-tetramethyl-indocarbocianina (DiI) a lipophilic carbocyanine that can make living or fixed cells. The DiI was chosen for this work because, among other factors, it is used for important studies of cell morphology. As a model for evaluating the quality of photooxidation of the apparatus constructed for this purpose, horizontal cells of human retina were elected in order to proceed with morphometric study these cells further, in view of the lack of studies with this approach in human retina. This study evaluated the quality of the DiI method photooxidation via LED and used the horizontal cells of the human retina as a model. The material was obtained from the Eye Bank of Ophir Loyola Hospital and sequence dissected, labeled with DiI crystals and photooxided with the LED device. The images resulting from the new method of lighting fluorescent tracer showed photooxidation of high-quality detailed neuronal morphology, similar to the results obtained in reactions of convencional photoconversion with a microscope, which indicates that the device maintains the efficiency of the photooxidation for revealing fine details of cellular morphology, including the advantages of larger areas of tissue processing and a considerable cost reduction for the use of microscopy dispensed into the process.
Acesso aberto (Open Access)
Caracterização vocal de pacientes portadores de Lúpus Eritematoso Sistêmico (LES)
(Universidade Federal do Pará, 2017-10-18) FREITAS, Milena Silva de; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Systemic lupus erythematosus (SLE) is a chronic disease characterized by progressive tissuedamage. In recent decades, novel treatments have greatly extended the life span of SLE patients. This creates a high demand for identifying the overarching symptoms associated with SLE and developing therapies that improve their life quality under chronic care. We hypothesized that SLE patients would present dysphonic symptoms. Given that voice disorders can reduce life quality, identifying a potential SLE-related dysphonia could be relevant for the appraisal and management of this disease. We measured objective vocal parameters and perceived vocal quality with the GRBAS (Grade, Roughness, Breathiness, Asthenia, Strain) scale in SLE patients and compared them to matched healthy controls. SLE patients also filled a questionnaire reporting perceived vocal deficits. SLE patients had significantly lower vocal intensity and harmonics to noise ratio, as well as increased jitter and shimmer. All subjective parameters of the GRBAS scale were significantly abnormal in SLE patients. Additionally, the vast majority of SLE patients (29/36) reported at least one perceived vocal deficit, with the most prevalent deficits being vocal fatigue (19/36) and hoarseness (17/36). Self-reported voice deficits were highly correlated with altered GRBAS scores. Additionally, tissue damage scores in different organ systems correlated with dysphonic symptoms, suggesting that some features of SLE-related dysphonia are due to tissue damage. Our results show that a large fraction of SLE patients suffers from perceivable dysphonia and may benefit from voice therapy in order to improve quality of life.
Acesso aberto (Open Access)
Complexidade semântica e habilidade de decodificação: um modelo quantitativo da compreensão de textos denotativos em língua portuguesa baseado na teoria da informação
(Universidade Federal do Pará, 2018-02-26) RIBEIRO, Louise Bogéa; RODRIGUES, Anderson Raiol; http://lattes.cnpq.br/4030747999301402; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Based on the principles of cognitive neuroscience and information theory, with emphasis on the work of Claude Shannon, a statistical analysis of 33,101 words was done from the collection of scientific texts of the Brazilian Digital Library Of Thesis And Dissertation and the Digital Library of USP, using the C # programming language and Microsoft Visual Studio 2012 as a code complement, including SQL Server Management Studio 2012 for database management, for the development of the information processing program titled CalcuLetra, with the purpose of measuring the difficulty of textual comprehension in Portuguese Language. From the premises that the learning of the meanings of letters, words and other symbols provides the development of the central nervous system of humans; that the reader's metacognitive behavior allows the response to stimuli coming from the reading process; And that the words of greatest occurrence in the bank represent those best known by their authors, the algorithm thus determines the degree of familiarity of the words according to the mathematical and statistical parameters of the bank. Therefore, when comparing non-literary or denotative texts with the probabilistic values found, it shows how comprehensible is the text inserted in the program, considering neurotypical readers and that its content has the necessary elements of textual cohesion, according to the grammatical rules of the language. Our results reveal groups of words that cause misunderstanding or make reading easier. Additionally, we show gaps in vocabulary and dictionary usage. Despite the preliminary findings, this study was more a proof of concept for the method employed, and demonstrated its potential for future research. The quantification model’s methodology presented in the present study can be adapted to other languages, and we hope it contributes to the development of objective evaluation of behavioral disorders (e.g., dyslexia), by quantitative classification of written incomprehension; and helps in the analysis of dissertations of vestibular, ENEM and other public examinations, whose evaluation is still of a subjective form.
Acesso aberto (Open Access)
Dinâmica de sucção disfuncional em lactentes com anquiloglossia
(Universidade Federal do Pará, 2023-11) CUNHA, Bruna Mendes Lourenço; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Introduction: Breastfeeding is widely recommended as an exclusive diet until 6 months of age and continued until two years or more. Its benefits are numerous and proven, including improving the immune system, a nutrient-rich diet and craniofacial development from the sucking movement. However, not all babies are able to breastfeed due to some difficulties, with ankyloglossia. Known as tongue tie, it can restrict tongue movements, making breastfeeding difficult and, in some cases, being responsible for early weaning. Objective: Compare infant suction in babies with and without ankyloglossia using a microprocessor-controlled pressure sensor coupled to a pacifier. Method: Fifty-five infants from 0 to 2 months of age underwent clinical examination for ankyloglossia, after which they were offered a silicone pacifier connected to the pressure acquisition device and suction activity was recorded. Thus, we extracted the frequency of sucks within a burst, the average suck duration, the burst duration, the number of sucks per burst, the maximum amplitude of sucks per burst and the inter-burst interval. Results: The key difference in newborns with ankyloglossia in relation to control was that they perform longer bursts of suction activity. Conclusion: The longer burst durations are likely a compensatory strategy and may underlie the pain reported by mothers during breastfeeding. We therefore propose a method for objectively quantifying some parameters of infant suction capacity and demonstrate its use in assisting the evaluation of ankyloglossia.
Acesso aberto (Open Access)
Dispositivo para avaliação postural em ambiente tridimensional
(Universidade Federal do Pará, 2018-07-17) NASCIMENTO, Nathalya Ingrid Cardoso do; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
The human posture can be defined as the balance between bone and muscle structures of the human body, through which it is possible to diagnose and treat various pathologies, so that science is in search of quantitative and precise methods for its analysis. A correct upright posture is considered a good health marker, making evaluation essential for a favorable therapeutic prognosis. As a result, it is necessary to develop new tools for postural evaluation with less mathematical and methodological errors to obtain accurate and reliable results for the clinic and research. The objective of this research is to present a new tool for postural evaluation, based on an electromechanical arm that allows measuring points in the three dimensional universe. The prototype was developed and improved in order to reduce errors at the time of data collection, for which a Hall effect sensor was used. The program allows to evaluate 24 relations between the points, in addition to the vertebral column analyzing the distance in millimeters and the angle in degrees between an anatomical accident and another in the anterior views, lateral right, posterior and left lateral. The initial tests were performed on the X and Y axes in an angular model, drawn at 10-degree angles and obtained low variability in their measurements, emphasizing the high accuracy of the prototype measuring system. Thus, it was possible to develop a new method of postural evaluation with low cost, high accuracy in its results for use in clinical practice, research and later insertion in the single health system.
Acesso aberto (Open Access)
Estruturas do olho do Macrobrachium amazonicum (Heller, 1862) (Crustacea, Decapoda, Palaemonidae): estudo utilizando microscopia óptica e de varredura.
(Universidade Federal do Pará, 2011-01-19) BOULHOSA, Silvio Márcio Pereira; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
The genus Macrobrachium contains more than 120 species and occurs in tropical and subtropical regions worldwide (VALENT, 1987). They are freshwater shrimp family Palaemonidae and order Decapoda (RAFINESQUE, 1815; LATREILLE, 1802). In Brazil there are 18 species, classified until now distributed throughout the Amazon Basin (MELO 2003). Among these, Macrobrachium amazonicum (HELLER, 1862) popularly known as shrimp quiet or shrimp cinnamon, widely used in shrimp farming. Crustaceans have sensory structures located on the cephalothorax, which allow then to receive the stimulus means to locate and capture food (BARNES, 1998). The compound eyes are present in all classes of crustacean. Thus, this research hypothesis was that in Macrobrachium amazonicum, these eyes are kind of overlay reflective, where the dioptric apparatus and rhabdom extend in layers and are separated by a clear non-pigmented area. In this work we have the following objectives: To evaluate the morphological aspects of the eye of Macrobrachium amazonicum scanning electron microscopy; characterize the morphology of the photoreceptor cells; describe the morphological structures of the eye of M. amazonicum; characterize the morphometric relationships between the eye and other structures of M. amazonicum. The animals were acquired in the district of ‘Mosqueiro’ in the rainy season, in March 2009 and March 2010, with fishermen in Santa Barbara city, the metropolitan area of Belém, and transported to the laboratory in polystyrene boxes, being held in quarantine in a vessel containing a solution of potassium permanganate at 1.3 mg / L (CARNEIRO et al., 2005). The animals were fixed in Davidson Karnovisky then the eyes of each animal were carefully sectioned and placed in plastic. Relations were performed in biometric analysis of variance with α = 0.05, was performed with Bio Stats 5.0 for the lengths of the side of the eye and anterior-posterior of the four morphotypes, and SigmaPlot 11.0 and simple linear regression for the variables eye total and cephalothorax. It was observed that the average size of the cephalothorax, the body, the eye is respectively: 1.03 mm, 70.62 mm and 4.52 mm, and the average weight of shrimp was 7.97 g. The structures of these maximum values recorded were 31.95 mm for the cephalothorax; 100.10 mm body size, 6.80 mm for the size of the eye and 20.54 g for the weight of the shrimp. Histological analysis identified the following structures (Fig. 13:14 AB): cornea, crystalline cone, pigment distal shaft of the cone, clear zone, cuticle, retina, rabdoma, optic nerve fibers and laminates. With respect to scanning electron microscopy, were selected to observe the main structures and especially the arrangement of ommatidia square cross section (Figs. 16 AB). The morphological and morphometric analysis (by histology and scanning electron microscopy) showed characteristics of Macrobrachium amazonicum adults from the metropolitan region of Belém. These findings include a reflective optical superposition eyes fitted with the perception of light stimuli.
Acesso aberto (Open Access)
Estudo do desenvolvimento auditivo da habilidade de fusão binaural
(Universidade Federal do Pará, 2022-04) ARAÚJO, Francisca Canindé Rosário da Silva; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Introduction: Binaural Interaction (BI) allows the introduction of auditory information (in the brain as a function of differences in perception of intensity or time of acoustic stimuli). Allows you to assess the action and integrated co- operative of the brainstem in lower understanding. As the maturation of the central nervous system occurs in the craniocaudal direction, the response to this ability may change during the course of development. Objectives: To normalize and compare the development with increasing age of response in the binaural diffusion test (BPT) with digital low-pass (LP) and high-pass (HP) filters in normative listeners. Methods: Prospective, cross-sectional and observational study. A total of 120 years were evaluated, in different age groups (6 to 8 years old, 10 to 12 years old, 14 to 16 years old and 20 to 30 years old) with TFB, filter at Fc 500/1700 Hz digital Finite Impulse Response type order 4096, with null phase and 5000 between 18 and 30 years with unfiltered speech material. Results: a progressive improvement in performance with increasing age (ANOVA (one-way): p<0.0001). There was a significant difference between the filtered words, age and the unfiltered p < (Dunnet: any filtered words,01). The difference between the age groups was significant (Tukey: p<0.01), less for the results obtained in the age groups of 6-8 and 10-12 years and of 14-16 and 18- 30 years. Discussion: IB is a skill that evolves with age development and NC fabrication. Final considerations: The interpretation of the TFB should take into account the performance by age group of the patients. This is important for future applications of these tests in people with Auditory Processing Disorder.
Acesso aberto (Open Access)
O fator de crescimento neuronal na infecção por Schistosoma mansoni: estudo molecular, imunoenzimático e morfométrico em modelo permissível e não permissível à infecção
(Universidade Federal do Pará, 2013-07-03) SANTOS, Daniel Valle Vasconcelos; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Schistosomiasis is a tropical disease caused by Schistosoma mansoni. His occurrence affects 110 million people worldwide. The deposition of eggs of the parasite may occur - in ectopic form – in the central nervous system (CNS) which leads to the formation of granulomas with consequent production of nerve growth factor (NGF). Since several studies have demonstrated the importance of NGF in the development of visual cortical pathways, our study aimed at evaluating the possible changes in the NGF concentratons in the visual system as well as the impact of this on the pyramidal cell morphology in two animal models. The change in concentration of the nerve growth factor as well as neuronal morphology were evaluated in suscetible and non-suscetible animals (mice and rats) to infection. We used 174 rats (Hooded Lister) and 135 albino mice bred and kept in cages and fed ad libitum. These animals were infected shortly after birth, with 50 cercariae. Seventy seven rats and 73 mice were inoculated with saline and constituted the control group of the study. The infection covered a period of 48 weeks . Samples of liver and visual cortex were removed, extracted and quantified with immunoassay kit (ChemiKineTM Nerve Growth Factor (NGF) Sandwich ELISA Kit - Chemicon International). For the morphometric analysis we used the pyramidal cells of the visual cortex layer IV marked by extracelular injection of biotinylated dextran (10,000 kDa). The results were expressed as mean ± standard deviation. We used Student t test to determine statistical differences between groups. The average value of NGF found in the visual cortex of rats infected was 39.2% higher than in the control group (infected: 400.9 ± 143.1 pg/ml, control: 288 ± 31.9 pg/mL, p < 0.0001). In liver samples, the increase was 28.9% higher in the infected group (infected: 340.9 ± 103.9 pg/mL, p < 0.01, control: 264.4 ± 38.6 pg/mL). No significant increase was detected within a week of infection. Among the mice group, the increase of NGF in the visual area was 94.1% (infected: 478.4 ± 284 pg/ml, p < 0.01; control: 246.5 ± 76.8 pg/ml). In the liver of these animals the increase was 138.7% (infected: 561.8 ± 260.7 pg/mL, p < 0.01, control: 301.3 ± 134.6 pg/mL). In mice group we found significant differences in dendritic parameters evaluated. The number of dendrites was 11.41% higher in the infected group than in the control (control: 25.28 ± 5.19; infected: 28.16 ± 7.45, p < 0.05). The total length of dendrites was also affected (control: 4916.52 ± 1492.65 μm; Infected: 5460.40 ± 1214.07 μm; p < 0.05), representing an increase of 11.06%. The total area of the dendritic receptive field was increased by 12.99% (control: 29.346,69 ± 11.298,62 μm2; Infected: 33.158,20 ± 7.758,31 μm2, p < 0.05) while the area had a somatic reduction of 13.61% (control: 119.38 ± 19.68 μm2; infected: 103.13 ± 24.69 μm2, p < 0.001). When we evaluated the effects of increased NGF in rats infected we did not observe significant differences in dendritic parameters analyzed, compared to the control group, except for an increase in the area of the neuronal body of approximately 21.18% (control: 132,20 ± 28.46 μm2; infected: 160.20 ± 31.63 μm2, p < 0.00001). This work showed that the reaction production of NGF in the CNS during infection with Schistosoma mansoni occurs in greater magnitude than permissible in the model in the model impermissible. We also demonstrated that in mice the effects on neuronal morphology is dramatically affected when the body is subjected to an increase in the concentration of NGF as a result of infection by Schistosoma mansoni. Given these data, studies evaluating the potential impact of visual effects and also in cell physiology caused by schistosomiasis infection becomes necessary to assess the actual damage caused by this pathological increase of nerve growth factor in the visual pathways of mammals.
Acesso aberto (Open Access)
Função de recuperação do nervo auditivo após doze meses de uso do implante coclear
(Universidade Federal do Pará, 2022-03) YAMAGUCHI, Cíntia Tizue; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Introduction: Cochlear implant is the standard treatment for severe and profound bilateral sensorineural hearing loss. To optimize the adaptation of this device, especially in patients who do not have the ability to refer to the parameters necessary for programming, such as children and people with associated disabilities, objective tests based on the action potential of auditory nerve fibers have been studied as possible predictors. of these parameters to be used in cochlear implant programming. The auditory nerve recovery function is a test that measures the time the auditory nerve needs to recover from a stimulus (leave the absolute refractory period) to receive new stimulation and possibly be more responsive to the auditory sensation offered by the cochlear implant. Objective: Through a specific software for cochlear implants, with the objective of measuring the time constant (τ) in the intraoperative period and in the postoperative period after 12 months of cochlear implant use in children. Method: We recruited children with cochlear implant and intraoperative neural response, evaluated the recovery function using commercially available cochlear implant software. Data were collected intraoperatively and repeated twice 12 months after surgery. Results: We found that the recovery time of the auditory nerve increases after 12 months of cochlear implant use. Our results also show that the profile of temporal responses is significantly higher in the postoperative measurement than in the intraoperative one. The test-retest reproducibility of the composite evoked action potential recordings proved to be reliable and stable. Conclusion: There was a change in 12 months of cochlear implant use, in relation to the same measure at the intraoperative moment in the subjects of this study. The intraoperative τ measurement was faster, however, in the postoperative period the mean showed higher τ values. However, the current level was different intraoperatively and 12 months after use, needing to be further explored. There was no statistical difference regarding the test-retest in the postoperative period, showing reliability and reproducibility of the measurement. Possibly it would be the beginning of the study of a responsiveness profile in relation to the recovery time of the auditory nerve.
Acesso aberto (Open Access)
Influência da facilitação neuromuscular proprioceptiva na alteração da fibra do músculo rectus femoris vista através da eletromiografia de superfície e dinamometria analógica
(Universidade Federal do Pará, 2013-09-23) PIN, Alessandro dos Santos; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
The PNF - Proprioceptive Neuromuscular Facilitation - is a technique that is increasingly being used in muscle training of healthy people and athletes. Studies have shown that resistance exercise, including PNF, are able to convert the trained muscle fiber type. This research aimed to verify the effectiveness of PNF in increased muscle strength and check for non-invasive methods would be indicative of conversion of muscle fiber type after training. A sample group of 22 young, female university students aged between 18 and 25 years, physically active, was divided into a control group (CG n = 10) and experimental group (EG n = 12). It was collected first the Maximum Volunteer Contraction MVC of Quadriceps muscle for analogic dynamometry and root mean square - RMS by surface electromyography (EMG) of all subjects. After the first data collecting GE conducted FNP based training in the dominant lower limb to 15 sessions in 5 weeks. Before the training sessions, new MVC and RMS data were collected of all samples. Regarding muscular strength, there was an increase in both groups, significant in CG (p< 0.01) and GE (p <0.05) for RMS and MVC time, there wasn´t significant increase in GE, but the interaction Vxt was significantly increased for this group. The results corroborate the literature by showing that muscles with a predominance of resistant fibers (fiber I / IIA) have greater contraction time with more electrical activation and that the FNP is able to type IIB fibers II A. In conclusion the training sample was efficient to increase muscle strength and EMG data presented show strong evidence of the conversion of muscle fibers trained.
Acesso aberto (Open Access)
Mapeamento de regiões de ativação cerebral durante tarefas deglutórias por imagens de ressonância magnética funcional
(Universidade Federal do Pará, 2013-11-25) SAAVEDRA, Adriana Di Paula Leopoldino; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
INTRODUCTION: Swallowing is a complex physiological process that happens for a motor sequence automatically regulated by a complex mechanism that neuromotor and neuromuscular starts consciously and is the result of anatomical and functional integrity of different facial structures. It is of utmost importance for the nutrition of the organism as a whole. And one of the biggest challenges in the field of science is to identify the neural substrates of physiological parameters, including the process of swallowing. The development of technology for functional neuroimaging in recent years is causing a rapid advancement in the knowledge of brain function, resulting in an explosion of new findings in neuroscience. OBJECTIVE: To map the regions of brain activation during swallowing phenomenon by examining fMRI. METHOD: The study included four females, aged between 18 and 30 years old without neurological, structural and food. After approval of the institution (Clínica Lobo), the Committee of Ethics and Research Institute of Health Sciences (ICS) and the written approval of each patient through the term of free and informed consent, underwent four swallowing tests deglutórias using the technique functional MRI. RESULTS: It was possible to determine the activation of the cerebral and cerebellar hemispheres and specific areas that compose them. Even with a small sample, the results of the individual analysis showed patterns according to the literature, together with new data. DISCUSSION: The cerebellum is responsible for the coordination of motor action and maintaining harmony of movements, position and balance of the bolus, the medulla along with the brainstem is the center of activity reflex that controls automatic functions or bodily responses like swallowing; midbrain is the part of the brain that coordinates the visual information; thalamus forwards almost any type of sensory information to specific areas of the cerebral cortex, hypothalamus, important in testing pleasure sensations, regulates the homeostatic functions of the body, taste , olfaction, salivation, interacting with the autonomic nervous system and the limbic system is connected to the control and direction of emotional reactions, under the action of the amygdala in processing odors and storing memory contents, here through the hippocampus. CONCLUSION: The act of swallowing is a complex process, activating many brain areas, among which we highlight the taste, mental / visual and olfactory and that starts long before the mechanical processes involved, as demonstrated by the cortical and subcortical areas activated. The olfactory area was most notably highlighted in the pictures taken by Rmf.
Acesso aberto (Open Access)
Parâmetros vocais acústicos de sujeitos do sexo masculino antes, durante e após o processo de muda vocal
(Universidade Federal do Pará, 2013-04-12) GUIMARÃES, Michelle Ferreira; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
The voice and the vocal ability are different in every individual and in every stage of life. There is a limited number of studies on the vocal quality of children and adolescents during puberty. The aim of this study was to quantify the following parameters of acoustic analysis of voice: fundamental frequency, jitter, shimmer, harmonic-to-noise ratio and intensity. To understand the relationship between the voice and the development of children and adolescents, these parameters were correlated with each other and with the degree of pubertal development in male subjects. Methods: The subjects of the study were 110 males, aged between 11 and 20 years, students from three Macapá schools, where the data collection was carried out. The subjects were divided into 4 groups, 32 subjects aged between 11 and 12 years composed the Group I, 29 subjects aged between 13 and 15 the Group II, the Group III consisted of 30 subjects aged between 16 and 18 years and Group IV by subjects aged between 19 and 20 years. All subjects underwent computer-based voice recording using a unidirectional microphone. The following phonation tasks were requested: saying sustained vowel /é/, counting from 1 to 10 and reading a pre-established paragraph. Then, the subjects were assessed by a physician for characterization of pubertal development according to the stages described by Tanner. The voice analysis was performed using the voice acoustic program Vox Metria ®. Results: The subjects showed the following F0 average during sustained vowel: 223.28 Hz, 249.86 Hz, 122.63 Hz and 127.61 Hz for Groups I, II, III and IV respectively. The mean of F0 during the sequential speech was 217.09 Hz, 246.18 Hz, 117.27 Hz and 123.42 for Groups I, II, III and IV respectively. Shimmer showed increased values for the four groups. Jitter, intensity and harmonic noise ratio remained within normal limits established by the acoustic voice program used. Most subjects were in G3 (n = 38, 34.5%) and G4 (n = 42, 38.2%) and P3 (n = 34, 31%) and P4 (n = 36 , 32.7%) stages of pubertal development. The degree of pubertal development was correlated with F0 during speech (p <0.001) and with F0 during sustained vowel (p <0.001) and this correlation was statistically significant between G2 and G5, and G3 and G5. Conclusion: Subjects aging up to 15 years showed acoustic vocal parameters typical of the childish voice. The decrease of F0 occurs between 16 and 20 years old, but the voice is still in process of stabilization, with increased values of shimmer. F0 is the only parameter correlated with the degree of pubertal development. The last stage of voice change, in the studied population, is a late event in relation to pubertal development.
Acesso aberto (Open Access)
Reabilitação de pacientes com implante coclear utilizando uma nova abordagem na análise da percepção auditiva
(Universidade Federal do Pará, 2018-03-28) RODRIGUES, Ana Paula Sirotheau Corrêa; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Communication is the way we have to interact with the world, this communication can be verbal or nonverbal. For the satisfactory progress of oral communication, it is necessary that the auditory pathway, from the central portion to the peripheral portion, is functioning properly. When there is a change in the auditory pathway, it is necessary to use devices that aid the perception of sound, among these instruments we highlight the cochlear implant, but for the proper use of this resource, an efficient rehabilitation is essential. Objective: To develop a cochlear implant simulator with the purpose of enabling the rehabilitator to identify the stimulus received by the hearing impaired, and thus to act in the schedules of the rehabilitation sessions, so that the sounds can be detected correctly, favoring the development or maintenance of the oral communication. Methods: For the development of the study, 3 groups of individuals were formed: the first group consisted of 6 implanted patients who received as pure sound stimulus. Groups 2 and 3 were composed of normo-listeners who received the sounds filtered by the cochlear implant simulator; group 2 with 22 individuals received filtered sounds for 22 activated channels (100%) and group 3 perceived filtered sounds with 17 channels activated (77%), corresponding to the number of channels activated in a cochlear implant. All participants were submitted to sessions with audiovisual stimuli in software running on a computer with a touch screen. The stimuli are 124 words extracted from a list used in the logoaudiometry exam, being 16 trisyllables, 46 disyllables and 62 monosyllables. The trisyllables words were generated synthetically and the others recorded in female voice of speech therapist. Results: It was observed that all groups presented greater difficulty in the detection of monosyllables, where the latency period was increased, and more errors occurred for sound perception, this is due to the reduction of the auditory track. Therefore, if there is an individualized rehabilitation treatment, it is believed that the development of the implanted hearing impaired is more efficient.
Acesso aberto (Open Access)
Registro do perfil de sucção em lactentes com e sem anquiloglossia por dispositivo microcontrolado
(Universidade Federal do Pará, 2018-08-09) BADARANE, Erika Baptista Luiz; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
In the first months of life, suction is the necessary function for oral feeding (VO) and adequate oral motor development. For this, it must be coordinated and harmonic, being necessary, among other factors: reflex of search and suction; lip sealing; adequate movement of the tongue and jaw; suction rhythm, sucking hatchings alternating with pauses; coordination between suction-swallowing-breathing. The adequate movement of the tongue has the function of performing the anterior (attached around the nipple) and posterior (attached to the soft palate and pharynx), nipple compression, and the organization and propulsion of the bolus. Considering that in the literature there is no consensus on the direct relationship between ankyloglossia and difficulty in breastfeeding or early weaning, the objective of this study is to evaluate the suckling profile in infants with and without ankyloglossia through a microcontroller device. For infants with and without ankyloglossia, a sterile silicone pacifier was attached to the suction device for two minutes. The device reads pressure variations from a sensor connected to a pacifier. The pressure data were processed using Kalman filters and neural network. The results showed that infants with and without ankyloglossia, when classified with a multilayer neural network - Perceptron Multilayer, with a topology of 5, 10 and 20 neurons in the hidden layer, did not present any segregation in the classified groups, that is, it was not found difference between them, with R (0.98) indicating a strong correlation between the groups. With this, we conclude that it is not possible to relate the presence of ankyloglossia with breastfeeding difficulty, emphasizing that this study used a more objective method of evaluation when compared to other studies in the literature.
Acesso aberto (Open Access)
Sistema robotizado para reabilitação de acometidos pelo acidente vascular encefálico - AVE
(Universidade Federal do Pará, 2017-08-03) ZAHLAN, Amir Samer; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Stroke is in the fifth place among the diseases that kill the most in the United States andis also one of the most leading causes of neurological disability in the world. Recently, robotic therapy has been more often used in the rehabilitation of individuals affected by stroke. In some cases, it proves more efficient than conventional therapy. There are few studies about the rehabilitation of the trunk and the equilibrium reactions with seated patient in individuals affected by stroke despite many daily activities are performed in this position. Robotic systems are used on stroke rehabilitation due it is possible to provide a high dosage and intensity of repetitive training aimed at specific activities. We propose a new platform to evaluate the reactions of individuals affected by stroke using robotic technologies. A model was constructed to simulate the movements of the system using pieces machined in plastic. A graphic control interface was developed to generate movement protocols where the physiotherapist can insert the parameters of these movements as commands, such as: angulation, speed and axis of the movement. It was possible to show that the platform moves accordingly to the programmed from repetitive tests of accelerometers data using moving average filter, expecting a precision of up to 1°. It is expected that this device may serve a large number of patients as an alternative in the rehabilitation of the stroke providing a safe and conducive environment for the rehabilitation.
Acesso aberto (Open Access)
Tecnologia de processamento de imagem para orientação de deficientes visuais dentro do espaço residencial
(Universidade Federal do Pará, 2024-03) ABDON, Leonardo Miranda; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
This work seeks to develop a product of assistive technology based on the Raspberry Pi platform in order to allow greater autonomy inside residential spaces for individuals with blindness. Therefore, it was assembled a wearable obstacle detection and path orienting system that uses a camera to capture images directed to the floor immediately in front of the user, in order to identify QR codes created specifically to generate responses within the written algorithm, so that responses are emitted through vibratory patterns in motors and recorded sentences through headphones. The system was developed in this way in order to allow autonomy in the choice of paths by the user with the aid of a low-cost assistive technology product, easily accessible and maintainable, and flexible in regard to the user's needs and preferences. The system’s capabilities were tested in a fixed setup inside a controlled study room without windows and in a movable setup in a controlled corridor, and the results were satisfactory regarding to the detection distances of paths and obstacles and the swiftness of emission of responses. Finally, a body vest made with bag straps was designed and the developed system was installed into it, to be used as a simple and nonintrusive wearable device.