Programa de Pós-Graduação em Neurociências e Biologia Celular - PPGNBC/ICB
URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/2374
O Programa de Pós-Graduação em Neurociências e Biologia Celular (PPGNBC) é parte integrante do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA), sendo constituído por: Mestrado e Doutorado em Neurociências e Biologia Celular, com área de concentração em Neurociências ou Biologia Celular.
Navegar
Navegando Programa de Pós-Graduação em Neurociências e Biologia Celular - PPGNBC/ICB por Afiliação "UEPA - Universidade do Estado do Pará"
Agora exibindo 1 - 3 de 3
- Resultados por página
- Opções de Ordenação
Item Acesso aberto (Open Access) Método baseado me cromatografia líquida de alta eficiência para analisar a atividade dos transportadores de GABA no sistema nervoso central(Universidade Federal do Pará, 2022-08) MORAES, Edinaldo Rogério da Silva; SILVA, Anderson Manoel Herculano Oliveira da; http://lattes.cnpq.br/8407177208423247The GATs are the membrane proteins responsible for the uptake of GABA in the central nervous system. Alterations in GAT activity are implicated in several neurological diseases, including retinopathies. The present study describes an alternative method to determine GAT activity in tissue preparations of the central nervous system, using high performance liquid chromatography (HPLC) with fluorescence detection. The GABA concentration in the medium was determined using the o-phthaldehyde (OPA)-derivation protocol validated by the Brazilian Health Regulatory Agency (ANVISA) and the United States Food and Drug Administration (US-FDA). The GAT activity in the retinal preparations was determined through the evaluation of the GABA uptake, which was measured by assessing the difference between the initial and final concentrations of GABA in the incubation médium (Δ𝐺𝑎𝑏𝑎 = [𝐺𝑎𝑏𝑎] 𝑡_0 − [𝐺𝑎𝑏𝑎] 𝑡_𝑒𝑛𝑑). The evaluation of the GAT kinetics returned values of Km = 382.5 ± 32.2 μM and Vmax = 34 nmol/mg of protein. The data also demonstrated that the GABA uptake was predominantly Na+ and temperature-dependent, and was also inhibited by incubation with nipecotic acid, a substrate of GABA transporters. Taken together, these findings confirm that our approach provided a specific measure of GAT activity in retinal tissue. The data presented here thus validate, for the first time, an alternative, simple and sensitive method for the evaluation of GAT activity using high performance chromatography on preparations of the central nervous system.Item Acesso aberto (Open Access) Pesquisa de hanseníase em doadores de sangue(Universidade Federal do Pará, 2024-11) JORGE, Erika Vanessa Oliveira; PALMEIRA, Mauricio Koury; http://lattes.cnpq.br/2785104508455046; SALGADO, Claudio Guedes; http://lattes.cnpq.br/2310734509396125Hansen’s disease (HD) is an infectious disease with active transmission in endemic areas such as Brazil, which accounted for over 90% of the new HD in the Americas in 2021, and the second highest in the global scale. Clinically is characterized by the appearance of nerve or skin lesions with altered sensitivity, that in advanced cases, can cause deformities and physical disabilities. Its transmission occurs mainly through Mycobacterium leprae, through respiratory droplets of sick people, and has a prolonged incubation period. The diagnosis of leprosy is clinical, with multidrug treatment PQT (polychemotherapy) being crucial to avoid the chain of disease transmission. Suspected cases should be attended by a qualified professional in basic health units or active case finding campaigns. Although leprosy patient is considered definitively unfit for blood donation process, according to Consolidation regulation nº 5, of October 3, 2017, leprosy investigation occurs only through a clinical interview, without laboratory evaluation. At Hemocenter from Pará, 500 donors were selected for the study and blood was collected for serological investigation of anti-PGL-I antibodies. Of the selected donors, 4% (20/500) had positive serological results greater than or equal to 0,750 optical density among only eight agreed to be clinically examined and underwent other laboratory tests: bacilloscopy and qPCR. Among donors with clinical evaluation, more than half were diagnosed with leprosy, 62.5% (5/8), with the following cases: one individual with the primarily neural clinical form, three with borderline-tuberculoid, one lepromatous. of peripheral blood, the presence of the bacillus in two individuals was confirmed, suggesting a possible route of transmission of the disease. We emphasize that the prevalence of subclinical leprosy infection due to its etiological agent among the study population is very high, but compatible with the hidden prevalence already recorded in the state of Pará.Item Acesso aberto (Open Access) Semelhanças cognitivas inesperadas entre idosos e jovens: variabilidade e desempenho cognitivo(Universidade Federal do Pará, 2023-08) JARDIM, Naina Yuki Vieira; TORRES, Natáli Valim Oliver Bento; http://lattes.cnpq.br/1927198788019996; https://orcid.org/0000-0003-0978-211X; DINIZ, Cristovam Wanderley Picanço; http://lattes.cnpq.br/2014918752636286Interindividual variability in cognitive performances has been investigated as they may provide important clues about the multivariate age-related cognitive decline. In the present work, we searched for cognitive variability, similarities, and differences between older and young people. For this, we used hierarchical cluster and canonical discriminant function analysis of cognitive scores using specific and sensitive tasks from the Cambridge Neuropsychology Test Automated Battery - CANTAB. Among 415 tested volunteers, three distinct cognitive groups were found, mainly based on working memory and episodic memory scores: group 1 (94% young adults) was composed almost exclusively of young adults, while groups 2 (82% older adults) and 3 (95% older adults) were composed predominantly of older adults. Although group 1was the youngest group with the highest level of education compared to the other groups, 18% of young people shared similar performances with older group 2 while 5% shared cognitive similarities with group 3. As compared to group 1, predominantly older groups 2 and 3 had equally lower scores in working memory, but as compared to group 3, group 2 showed greater performances in reaction time, sustained attention, and episodic memory. When the hierarchical cluster and discriminant function analyzes were limited to the same age group, we found 4 and 5 distinct clusters among young adults and older people respectively. Episodic memory, sustained attention, and reaction time most contributed to group formation in the older, while working memory and sustained attention contributed to cluster formation of young adults. Cognitive variability across subjects showed significant dispersion in rapid visual processing, spatial working memory, reaction time, and paired associated learning. The comparative analysis of these differences showed that they do not occur in the same direction and magnitude between individuals, cognitive domains, and tasks. We found that older adults with greater education and a more active lifestyle must have greater cognitive reserve and, therefore, deviated less from the reference group of young adults. Taken together, our data highlight the importance of studying variability as an instrument for the early detection of subtle cognitive declines and to interpret results that deviate from normality.