Teses em Ciência Animal (Doutorado) - PPGCAN/Castanhal
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/4665
O Doutorado em Ciência Animal teve início em 2008, com início da primeira turma em 2009 junto à CAPES/MEC e funciona no Programa de Pós-Graduação em Ciência Animal (PPGCAN) do Campus Universitário de Castanhal (CCAST) da Universidade Federal do Pará (UFPA), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) e Universidade Federal Rural da Amazônia (UFRA).
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Navegando Teses em Ciência Animal (Doutorado) - PPGCAN/Castanhal por Autor "DOMINGUES, Sheyla Farhayldes Souza"
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Item Acesso aberto (Open Access) Análise imunohistológica e desenvolvimento folicular após auto-transplante de tecido ovariano de macaca de cheiro Saimiri collinsi(Universidade Federal do Pará, 2014-12-11) SCALERCIO, Sarah Raphaella Rocha de Azevedo; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149; SANTOS, Regiane Rodrigues dos; http://lattes.cnpq.br/0500967766886604The main objective of the present work was to study ovarian folliculogenesis in Saimiri sciureus. Therefore, we evaluated the expression of intra-ovarian factors related with the follicular development, inhibition and cell proliferation, as well as investigation the effects of pretreatment with vitamin E analogue, Trolox, on the viability and follicular function, apoptosis index, cell proliferation, vascularization and fibrosis after auto-transplanted of fresh ovarian tissue. It was possible for the first time imuno localize proteins expressed as: growth and differentiation factor -9 (GDF -9), c- kit/Kit Ligand and Ki-67 and confirm the expression of anti-Mullerian Hormone (AMH) in different stages of follicular development in squirrel monkey ovary. Regarding pre-incubation with the Trolox antioxidant, it can improves follicular survival after autotransplantation, decrease apoptosis rates in stromal cells, although increase areas of fibrosis after graft. Our results suggest that fresh ovarian tissue can be grafted and incubated without major impact on early follicular growth and morphology after subcutaneous autotransplantation for a short-term. We believe that our results provide an important contribution to understanding the process of folliculogenesis in neotropical monkeys. This knowledge is an important tool to evaluate follicular viability and functionality after cryopreservation of ovarian tissue transplantation and in vitro culture of early follicles, especially for animals at risk of extinction and women that are undergoing to chemotherapy and radiation with risk of loss fertility.Item Acesso aberto (Open Access) Biometria testicular, caracterização e congelamento de sêmen de macacos-de-cheiro de vida livre (Saimiri vanzolinii, S. cassiquiarensis e S. macrodon) e cativeiro (S. Collinsi) em água de coco em pó (ACP-118®)(Universidade Federal do Pará, 2014-06-24) OLIVEIRA, Karol Guimarães; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149Recently, Saimiri sciureus taxon (squirrel monkey) was revised and some subspecies are now classified by some authors as species, for example: Saimiri collinsi, S. cassiquiarensis and S. macrodon. Phenotypically these species are well determined. However, information on reproductive aspects is still quite scarce. The objective of the first phase of this study was to describe and correlate the testis with the seminal quality from captive (S. collinsi) and free-living (S. vanzolinii, S. cassiquiarensis and S. macrodon) squirrel monkeys. Length, width, height, circumference and testicular volume were measured as well as motility, vigour, plasma membrane integrity and morphology, for correlation data. No difference was observed between the right and left testicles within species, neither difference between species concerning to testicular measures. Semen, which was collected by electroejaculation with rectal probe, was constituted by a coagulated fraction, a liquid fraction or both. Seminal quality was similar among species. There is no correlation between testicular volume and seminal volume (of liquid or coagulated fraction) in S. collinsi and S. vanzolinii. The second phase of this work aimed to propose a semen freezing protocol in an extender based in ACP- 118® (powdered coconut water), testing two glycerol concentrations (1.5 and 3%) for captive S. collinsi. The third phase aimed to test a semen cooling protocol without addition of egg yolk (extracellular cryoprotector) followed by freezing with the best glycerol concentration observed in the preceding experiment and apply these protocols, previously tested in captive S. collinsi, in free-living males from S. vanzolinii, S. cassiquiarensis and S. macrodon in field conditions. Semen from S. collinsi could be cooled in ACP-118® without egg yolk. The freezing protocol developed proved to be useful for semen cryopreservation of S. Collinsi, although requiring improved to allow better maintenance of sperm quality after thawing. The procedure proved to be also applicable under field conditions on free-living animals, providing very encouraging results for the formation of genebanks for species vulnerable to extinction as the S. vanzolinii.Item Acesso aberto (Open Access) Incubação de ovos e criação de filhotes de guará (Eudocimus ruber) no parque Mangal das Garças: uma ferramenta para a conservação da espécie(Universidade Federal do Pará, 2015-06-12) MIRANDA, Stefânia Araújo; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149The population of scarlet ibis is considered to be extinct in several regions. Captive breeding techniques are complementary tools for species conservation. The objectives were to develop a feeding protocol and an ambient environment for the rearing of scarlet ibis chicks, to intensify reproduction in captivity and increase the number of ibises in the colony, describe the developmental stages of chicks and develop growth estimation equations. The chicks were fed with three diets: Diet C (commercial feed), S (commercial ration and shrimp) and F (commercial ration and fish). The body condition and weight were taken daily, and the body measurements every seven days. The mortality rate under Diet C was 100%, and 0% under Diets S and F. The mean body weight and bones lengths were higher with Diet F and the mean length of the unpigmented portion of the beak did not differ among the diets (P<0.05). Diet F had higher nutritional contents. Rearing was artificial during year I and natural during year II at one of the areas of the park. The limbs development and body condition were evaluated daily, and the weight every seven days during artificial rearing. The mortality rate was lower and the number of births and survival rate were higher during artificial rearing. All of the young exhibited weight gain (P<0.05), good body condition and no dystrophies were observed. The development stages of chicks and the weight were monitored daily, and the measurements of the beak and bones were taken every seven days. The eyes opened on day 4.73±0.12, the nestlings moved within the nest on day 6.31±0.18 and left the nest on day 15.3±0.68. The means weight and measurements of the bones increased (P<0.05). The means of beak despigmentation decreased (P<0.05). The beak pigmentation started on day 7º and on day 35º was completely pigmented. The correlation between all parameters and the determination coefficients of regression equations were high. Diet F was found to be best for the artificial rearing. The artificial rearing protocol provided an adequate environment and feeding, intensified the reproduction and increased the number of scarlet ibises. Growth estimation equations were developed to assess the chicks growth and was possible to describe the developmental stages. Beak pigmentation was found to be a useful parameter for estimating the age.