Teses em Ciência Animal (Doutorado) - PPGCAN/Castanhal
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/4665
O Doutorado em Ciência Animal teve início em 2008, com início da primeira turma em 2009 junto à CAPES/MEC e funciona no Programa de Pós-Graduação em Ciência Animal (PPGCAN) do Campus Universitário de Castanhal (CCAST) da Universidade Federal do Pará (UFPA), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) e Universidade Federal Rural da Amazônia (UFRA).
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Navegando Teses em Ciência Animal (Doutorado) - PPGCAN/Castanhal por Autor "FREITAS, José de Arimatéa"
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Item Acesso aberto (Open Access) Avaliação dos perigos microbiológicos em uma indústria de beneficiamento de pescados e sugestão de um sistema de gestão de qualidade(Universidade Federal do Pará, 2012-09-21) LIMA, Consuelo Lucia Sousa de; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769; FREITAS, José de Arimatéa; http://lattes.cnpq.br/2382745365421156From among products of animal origin, fish are the most susceptible to deterioration and may represent a risk to health if they are contaminated by pathogenic micro-organisms. Therefore the fish industries must take special care during ali stages of processing. This study evaluated microbiological hazards in the processing line and products in a fish industry and proposes its adaptation to the necessary prerequisites for implementation of Hazard Analysis and Critical Control Points (HACCP) system. Were carried out microbiological and physicochemical analysis of 144 samples (fillets and flitch) of frozen fish of different species collected directly in the industry, were also submitted to microbiological testing 12 ice samples, 24 water samples and 340 samples from surfaces (equipment, tools and gloves handlers). The Good Manufacturing Practices (GMP) were evaluated using a checklist applied during five surveys in the industry. Trainings were carried out and evaluated temperatures of raw materials and semi finished products. It was then proposed a HACCP plan for processing line of frozen fish fillet. Among the pathogens evaluated only coagulase positive staphylococci were detected in 2.7% of fish samples, within the limit established by Brazilian law, but above the values established by national legislation for pH and BVT were detected in 18.1% and 3.5% of the samples, respectively. The samples of ice and water were not detected coliforms at 35°C and 45°C. From samples of equipment and utensils analyzeds, 50% and 81.82% were within the recommendations established by OPAS of 1.7 log CFU/cm2 for aerobic mesophilic and coliforms at 45°C, the first and second sampling period, respectively. The gloves, the first sampling period showed counts above 4 log CFU / glove of mesophilic aerobic microorganisms in 76.67% of the samples and in the second period in only 21.67%. In the evaluation of GMP in the first two surveys the industry was classified as High Risk for having submitted the conformities percentage below 50%, and as Medium Risk in subsequent surveys, for answering 51-75% of the recommended items in the check list. With these results it was found that GMPs are not fully implemented in the industry analized, detecting several non-conformities. The development of the HACCP plan was carried out according to the following steps: formation of the team, product description, development of production flowchart, hazard analysis, identification of Critical Control Points (CCPs), establishment of critical limits and corrective actions, and monitoring, records and verification procedures. The proposed implementation of the HACCP plan is applicable and may contribute significantly to the development of fish products with higher quality and food safety.Item Acesso aberto (Open Access) Distribuição espacial de anticorpos IgG para Toxoplasma gondii em um estudo soroepidemiológico realizado em bovídeos no estado do Pará(Universidade Federal do Pará, 2014) OLIVEIRA, Jefferson Pinto de; CASSEB, Alexandre do Rosário; http://lattes.cnpq.br/6525374874057337; FREITAS, José de Arimatéa; http://lattes.cnpq.br/2382745365421156This study aims to determine the prevalence of antibodies immunoglobulin class G (IgG) specific of this disease in bovine created, by contributing to the understanding of the epidemiology of this zoonosis in the state of Pará. The study was conducted in all Para meso, they were harvested blood samples from animals in 2070, with 1750 and 320 bovine and buffalo, respectively, coming from 52 municipalities and 100 properties, a survey of the epidemiological aspects influencing the onset of the infectious agent. Sera of animals underwent the test of ELISA Kit – Enzyme-Linked Immunosorbent Assay and indirect immunofluorescence - IFA for the detection of specific IgG antibodies to T. gondii cattle and buffalo the Imunodot® company. The animals were considered positive in the IFAT test from the dilution 1:64. It was considered statistically significant difference p 0.005. The buffaloes had higher seropositivity that the bovine, as well as the IFA test was higher than the ELISA test, and Mesoregion more seropositive was the Lower Amazon. There were no significant differences in the frequency of T. gondii, in relation to sex, type of exploitation, but no significant association for age, size of property, number of animals in the property, creation cycle, and the properties that presented greater amount of cats and dogs were the ones that had higher sororreagência the anti - T. gondii.Item Acesso aberto (Open Access) Fauna microparasitária de Brachyplatystoma rousseauxii e Mugil curema desembarcados na Amazônia oriental(Universidade Federal do Pará, 2013-11-29) DIAS, Lilian de Nazaré Santos; MATOS, Edilson Rodrigues; http://lattes.cnpq.br/7895814591867510; PAIVA, Rosildo Santos; http://lattes.cnpq.br/0510818763187669; FREITAS, José de Arimatéa; http://lattes.cnpq.br/2382745365421156The gilthead bream Brachyplatystoma rousseauxii and white mullet Mugil curema are species of fish consumption and considerable commercial value found in estuarine cost of Pará The microparasites phyla Apicomplexa, Microspora and Myxozoa are organisms that can be found parasitizing vertebrates and invertebrates, including the fish, some pathogenic potential and even zoonotic and economic impact. To discover the fauna microparasites affecting fish , 62 specimens of B. rousseauxii and 58 M. curema estuarna captured on the coast of the city of Vigia de Nazare and Mosqueiro District, in Belem, Para state were examined as methods and techniques for morphological analysis (microscopy light), ultrastructural (transmission electron microscopy, TEM) and molecular biology (phylogenetic analysis). It was observed that there are three phyla (three in B. rousseauxii and two in M. curema) captured hosts in the two locations , plus the largest parasitic index was determined in specimens of both captured on the coast of the estuarine Vigia de Nazare , higher incidence of co microparasites phyla Apicomplexa and Myxozoa, beyond multiparasitism in B. rousseauxii Morphological analysis revealed the presence of Calyptospora sp. (Apicomplexa), Ellipsomyxa sp., Henneguya sp., Myxobolus sp. and Meglishcha sp. (Myxozoa) and Kabatana sp. (Microspora) in B. rousseauxii and Ellipsomyxa sp., Myxobolus sp. and Microspora phylum microparasito in M. curema. The data analysis of morphological and ultrastructural Myxospora found in B. rousseauxii and M. curema are suggestive of new species microparasites these two hosts. Phylogenetic analysis of the data did not provide results that allow the classification of Kabatana sp. (Microspora) and Henneguya sp. (Myxozoa) found parasitizing B. rousseauxii microparasites as new species in this host due to low bootstrap value, but by analyzing the distance p was possible to suggest that these are new species. Studies about microparasites in Amazonian fish are needed for the knowledge of the occurrences, characterization d new species, the pathogenic potential hosts and possible risk to the consumer.