Dissertações em Doenças Tropicais (Mestrado) - PPGDT/NMT
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/3559
O Mestrado Acadêmico em Doenças Tropicais iniciou em 2004 e pertence ao Programa de Pós-Graduação em Doenças Tropicais do Núcleo de Medicina Tropical (NMT) da Universidade Federal do Pará (UFPA).
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Item Acesso aberto (Open Access) Avaliação da susceptibilidade de camundongos BALB/c e Swiss, hamster e Proechimys roberti à infecção por Leishmania (Viannia) Naiffi e Leishmania (Viannia) Lindenbergi(Universidade Federal do Pará, 2005) SODRÉ, Roberta Nice Salgado; ISHIKAWA, Edna Aoba Yassui; http://lattes.cnpq.br/3074963539505872Leishmania (Viannia) naiffi and Leishmania (Viannia) lindenbergi are species that cause cutaneous leishmaniasis in Amazonia and present great similarity in its isoenzymatic profile, monoclonals antibodies and production of unapparent infection in hamsters. The fact of not having a highly susceptible experimental model to the infection for L. (V.) naiffi and L. (V.) lindenbergi, the objective of this study was evaluate the susceptibility of BALB/c and Swiss mice, hamsters and Proechimys roberti to the infection for those two species. It was prepared inoculums with salivary glands and without glands for each group of animals, associated to the promastigotes. The experimental animals, of both sexes, were inoculated intradermally in the dorsal surface of back feet and they were observed for 90 days. In the period of 30, 60 and 90 days after inoculation, the animals were sacrificed and different fragments of skin of the inoculation place were used in the culture, microscopic exam and polymerase chain reaction (PCR). It was not possible to observe lesions in the animals inoculated with L. (V.) naiffi and L. (V.) lindenbergi even at the presence or absence of salivary glands. As well as, forms amastigotes during 30, 60 and 90 days after the inoculation. In the culture, all the animals inoculated with L. (V.) lindenbergi haven’t developed promastigotes. For the other hand, BALB/c mice inoculated with L. (V.) naiffi presented positively when sacrificed 30 days after inoculation. PCR presented low sensibility compared to the culture. This way, we concluded that L. (V.) naiffi and L. (V.) lindenbergi is species that present low infectivity and none of the animals used in the study experimental model can be considered highly susceptible to the infection for those two species.Item Acesso aberto (Open Access) Avaliação externa da qualidade das baciloscopias para tuberculose, realizadas nas unidades laboratoriais públicas no município de Belém (Pará)(Universidade Federal do Pará, 2010-10-04) BRITO, Michele Lima de; LIMA, Karla Valéria Batista; http://lattes.cnpq.br/9795461154139260The sputum examination is the most widely used for diagnosis and control of tuberculosis (TB), as a method of simple execution, low cost, but has low sensitivity and specificity. For the effectiveness and efficiency of this method are met, it is necessary for the establishment and application of standard rules of execution and reading of slides. Thus, it is essential to know the quality of smear microscopy available in a laboratory network. The aim of this study was to evaluate the quality of sputum for diagnosis and control of TB conducted in Units Laboratory (UL) belonging to the public laboratories in Belém, Pará, in 2007, 2008 and 2009. The evaluation was divided into three stages, corresponding to the year of manufacture of the sputum blades. Participants UL 18 that sent regularly to the Central Laboratory of Para, all smear slides prepared by them for External Quality Assessment of Smear (AEQB), which uses the method of blind rereading of smears. This method involves a technical evaluation of the smears, describing them as "Adequate" and "Inappropriate", besides the analysis of discrepant results False Positive (FP) and false negative (FN). During the study period, 4117 samples were processed by UL. In the first stage of evaluation, all 18 UL had blades that were classified as inadequate, and this number decreased to 12 UL in the 2nd stage of evaluation and UL for six at the 3rd step. Regarding disagreements, disagreements had 03 UL 03 UL FP and FN. The results obtained in this study reinforce the need to maintain and expand AEQB for all UL performing smear in the network of laboratories.