Programa de Pós-Graduação em Farmacologia e Bioquímica - FARMABIO/ICB

URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/13298

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Avaliação anticonvulsivante e pró-convulsivante de óleos essenciais de Lippia origanoides e Rosmarinus officinalis em ratos: um estudoeletrofisiológico
(Universidade Federal do Pará, 2025-05) ARAÚJO, Daniella Bastos de; HAMOY, Moisés; http://lattes.cnpq.br/4523340329253911; https://orcid.org/0000-0002-2931-4324
Epilepsy is a neuronal disorder characterized by abnormal brain excitability, leading to seizures. Only about 66% of epileptic patients respond adequately to treatment with existing conventional anticonvulsants, making it necessary to investigate new antiepileptic drugs. The growing research on natural products and their pharmacological properties has become increasingly promising, particularly in the study of essential oils, already widely used in popular culture for the treatment of several diseases. The present studies evaluated the anti- and pro-convulsant effects of the essential oils of Lippia origanoides (LOEO) and Rosmarinus officinalis (EORO) in Wistar rats. We evaluated the essential oil of Lippia origanoides (LOEO) (100 mg/kg i. p.) in comparison with diazepam (DZP) (5 mg/kg i. p.) and the combined administration of these two substances to control seizures induced by pentylenetetrazole (PTZ) (60 mg/kg i. p.). This evaluation was carried out using 108 male Wistar rats, which were divided into two experiments. Experiment 1 – Behavioral evaluation and Experiment 2 – Electrocorticographic evaluation. With rosemary essential oil, we evaluated high doses in 54 Wistar rats, weighing between 180 and 200 g. The study consisted of three experiments: 1) behavioral monitoring of the animals after administration of 500 mg/kg i.p.; 2) electrocorticographic recordings after drug administration; 3) reaction to anticonvulsant drugs, where phenytoin, phenobarbital and diazepam (10 mg/kg i.p.) were administered. With LOEO, the animals presented a more intense decrease in respiratory rate when combined with LOEO + DZP. EEG recordings showed a reduction in firing amplitude in the groups treated with LOEO. Combined treatment with diazepam resulted in increased anticonvulsant effects, while with EORO, the results demonstrated an increase in the latency time for the onset of isolated clonic seizures without loss of the postural reflex. The animals showed a more intense decrease in respiratory rate when combined with LOEO + DZP. EEG recordings showed a reduction in firing amplitude in the groups treated with LOEO. Combined treatment with diazepam resulted in increased anticonvulsant effects. Treatment with LOEO was effective in controlling seizures, and its combination with diazepam may represent a future option for the treatment of difficult-to-control seizures, while treatment with EORO demonstrated an excitatory activity related to the reduction of GABAergic activity.
Acesso aberto (Open Access)
Efeitos citotóxicos e mecanismo de ação da eleuterina isolada de Eleutherine plicata em modelo in vitro de células c6
(Universidade Federal do Pará, 2024-05) SHINKAI, Victória Mae Tsuruzaki; NASCIMENTO, José Luiz Martins do; http://lattes.cnpq.br/7216249286784978; https://orcid.org/0000-0003-3647-9124
Glioblastoma multiforme (GBM) is the most prevalent malignant primary tumor of the central nervous system (CNS). GBM cells are characterized by rapid proliferation and aggressive migration. There is growing demand for new therapies to treat this tumor, due to current therapeutic limitations. Quinone derivatives from plants have received increased interest as potential antiglioma drugs due to their diverse pharmacological activities such as inhibition of cell growth, inflammation, tumor invasion and promotion of tumor regression. The herb Eleutherine plicata, popularly known as Marupazinho, is widely used in popular medicine due to its pharmacological properties, containing quinone derivatives, more specifically naphthoquinones. Previous studies have demonstrated the antiglioma activity of Eleutherine plicata, which is related to three main naphthoquinone compounds – eleutherine, isoeleutherine and eleutherol – but mechanism of action remains unclear. Thus, the objective of this study was to investigate the potential cytotoxic and antiproliferative effect of eleutherin in an in vitro model of glioblastoma (C6 lineage). In vitro cytotoxicity was assessed by the MTT assay; Morphological changes were assessed by phase contrast microscopy. Apoptosis was determined by the annexin V-FITCpropidium iodide assay, and antiproliferative effects were assessed by the colony formation assay. Protein kinase B (AKT/pAKT) expression was measured by western blot, and telomerase reverse transcriptase mRNA was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The results obtained indicated that eleutherin, isolated from the Hexanic fraction, had a cytotoxic effect on the C6 lineage. Structural changes were observed by image capture, with a significant reduction in colony formation, induction of apoptosis, inhibition of pAKT and reduction in telomerase expression after treatment. Thus, our study showed that the eleuterin molecule has cytotoxic activity in C6 lineage glioma.

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