Teses em Ciência Animal (Doutorado) - PPGCAN/Castanhal

URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/4665

O Doutorado em Ciência Animal teve início em 2008, com início da primeira turma em 2009 junto à CAPES/MEC e funciona no Programa de Pós-Graduação em Ciência Animal (PPGCAN) do Campus Universitário de Castanhal (CCAST) da Universidade Federal do Pará (UFPA), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) e Universidade Federal Rural da Amazônia (UFRA).

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Acesso aberto (Open Access)
Infecção por Brucella abortus em búfalas (Bubalus bubalis)
(Universidade Federal do Pará, 2016) SOUSA, Melina Garcia Saraiva de; SALVARANI, Felipe Masiero; http://lattes.cnpq.br/3339195791140438; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557
The objective of this study was to detect Brucella abortus and injuries caused by the bacteria in fetal membranes and fetuses, and in lymph nodes of buffaloes as well as to describe the lesions caused. Twenty buffaloes serologically positive for brucellosis were used and subjected to stamping for collection of material from the closed uterus of several months gestation. Fragments of lymph nodes were collected. Fetal age was determined by ultrasound examination and the size of fetuses was measured at necropsy. The samples were subjected to histopathology and qPCR. From the second month of pregnancy on it was possible to detect the presence of B. abortus DNA in amniotic fluid, allantoic liquid and uterus, and from the fifth month on in placenta, heart, spleen, kidney, lung, intestine, liver and lymph nodes of the fetuses. The main pathological findings were fibrinous suppurative necrotic placentitis, and chronic endometritis. The detection of B. abortus DNA in the lymph nodes was checked from the fourth month of pregnancy in seven buffaloes and in a post-calving buffalo. Acute to chronic lymphadenitis was histologically diagnosed. B. abortus DNA was detected in all evaluated groups of lymph nodes; the mammary lymph nodes were the most affected.
Acesso aberto (Open Access)
Intoxicação por sal em ovinos e caprinos no estado do Pará
(Universidade Federal do Pará, 2014-06-30) DUARTE, Marcos Dutra; BARBOSA NETO, José Diomedes; http://lattes.cnpq.br/1516707357889557
This paper reports the first outbreaks of salt poisoning in sheep and goats in Brazil on a farm in the state of Pará. The evaluation of the facilities and the handling indicates as predisposing factors the excessive intake of mineral mixture and water restriction. The first outbreak occurred in sheep, eight animals (1,46%) were affected from a total of 545 animals and four (50%) of them died. The second and third outbreaks occurred in a herd of 191 goats. In the second outbreak, four animals (2,09) were affected and none died. In the third outbreak, eight goats (4,19%) were affected and two (25%) of them died. In sheep the main clinical signs were recumbency, decrease or absence of cutaneous sensibility, absence of auricular, palpebral and menace reflexes, mydriasis, nystagmus, opisthotonus, spasticity of limbs, somnolence and stupor. Beside this signs observed there were bloat, diarrhea, tachypnea, tachycardia, dehydration and polyuria. The clinical course of the disease in sheep that died ranged from 2 and a half hours to 48 hours. The main clinical signs in the goats were muscle weakness, abnormal posture of the head and neck such us staying with his head down, head and neck rotation, neck extension and lateral deviation of the neck, sternal and lateral recumbency, mydriasis, absence of pupillary reflex to light, stay with pelvic and thoracic limbs abduced or in broad-based, increased flexor reflex, polyuria and intense thirst were observed. They were observed also rumen hypomotility, tachycardia, decreased skin turgor. The clinical course in goats of second outbreak varied from 4 to 24 hours. In the goats of third outbreak who died the evolution ranged by six to eight hours and two to three days in the goats who have recovered. The medium of serum concentration of sodium and potassium of 31 sheep from the same group affected on samples collected during the outbreak revealed hypernatremia (190 mEq/l) and hyperkalemia (8.2 mEq/l), respectively. The mean serum concentrations of sodium and potassium of 36 goats from the same lot of animals affected in the second outbreak in samples taken two days before the onset of clinical signs revealed hypernatremia (167 mEq/l) and the average level of potassium (4.7 mEq/l) within the reference values for the species. The averages of these same parameters in samples from goats in the third outbreak with clinical signs of intoxication (143,7 mEq/l for sodium and 3.9 mEq/l for potassium) were within the reference values. However, the averages of these same parameters in samples of goats in the third outbreak, with clinical signs of intoxication (143.7 mEq/l for sodium and 3.9 mEq/l for potassium), were within the reference values. At necropsy in only one of four sheep there was flattening of the cerebral gyri. Microscopically in this sheep there was a vacuolization of the neuropil in the cerebral cortex with moderate intensity in the intermediary surface and mild on the superficial, with increased of perineural and perivascular spaces. In these areas there was still markedly swollen nucleus of astrocytes and acute neuronal necrosis. Noteworthy macroscopic or microscopic changes were not observed in the goats. The dosages of sodium showed high values in the brain of the poisoning animals, in an ovine value of 3,513 ppm and in two goats, values of 3,703 and 3,675 ppm. Five goats of the third outbreak were treated with dexamethasone and thiamine, intramuscularly, twice daily for two days, and water was offered in small amounts three times a day. All treated animals were recovered in three days. The diagnosis of salt poisoning was based on epidemiology, clinical signs, in the lesions, in the dosages of sodium in serum and brain and in the response to treatment in five goats.

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