Navegando por CNPq "CNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA::FISIOLOGIA DOS GRUPOS RECENTES"

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Atributos funcionais como características distintivas de comunidades: o que diferencia anuros do Cerrado e da Caatinga?
(Universidade Federal do Pará, 2019-01) SANTOS, Mayra Caroliny de Oliveira; COSTA, Maria Cristina dos Santos; http://lattes.cnpq.br/1580962389416378
Environmental conditions influence the distribution of organisms and modify functional characteristics. These characteristics may be morphological, behavioral, and physiological with a function. We evaluated the taxonomic and functional diversity of anurans in Cerrado and Caatinga environments in Piauí, Brazil. The anurans were collected in Floriano Municipality in the Cerrado area with cerradão phytophysiognomy and in Alvorada do Gurguéia municipality in the Caatinga with a arboreal caatinga phytophysiognomy. The sampling method was visual encounter and acoustic survey between January and April of 2018. We established six plots at least 500 meters distant between them in each location. The species composition was verified by PCoA and PERMANOVA analysis. For functional diversity, it was used the Rao’s quadratic entropy. The RLQ and the fourth-corner method related the traits to the environmental characteristics. The results showed separation and difference in the composition of the species according to the different areas. Cerrado and Caatinga phytophysiognomies showed differences in functional diversity values of anurans. Cerrado area had relation with the temperature and precipitation and the Caatinga area with the litter. The anuran composition was affected by environmental variables, but the influence on functional traits composition was not significant. Then, the composition was influenced by the environment and the distinctions between phytophysiognomies, such as the rainy season and quantity of microhabitats, were important for the differences in functional traits of anurans species, and consequently on functional diversity
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Ensaios estereológicos e morfologia tridimensional na formação hipocampal de aves migratórias marinhas: análise quantitativa da imunomarcação seletiva de neurônios e micróglia em Calidris pusilla e Actitis macularia
(Universidade Federal do Pará, 2013-08-14) DINIZ, Cristovam Guerreiro; SHERRY, David Francis; DINIZ, Cristovam Wanderley Picanço; http://lattes.cnpq.br/2014918752636286
The aim of this report is to describe the hippocampal formation of two migratory birds, Calidris pusilla and Actitis macularia, that leave the cold regions of the Canadian Tundra, escaping the winter, towards the coast of South America and the Caribbean Islands where they remain until winter ends returning to the Northern hemisphere. We intend to describe the qualitative and quantitative morphological organization of the hippocampal formation, using cytoarchitetonics with cresyl violet and immunostaining for neurons and microglia, followed by stereological estimates of the total number of cells identified with selective markers. We intend as well to compare the three-dimensional morphology of hippocampal microglia of these species with those of the dentate gyrus of Wistar rats and Capuchin monkey. The shorebirds used in the characterization were captured in Canela Island, Bragança City, State of Pará, Brazil (0°47'21.95"S and 46°43'7.34"W), as well as in Canada, in the Bay of Fundy, near Johnson's Mills, New Brunswick (45° 50' 19.3" N 64° 31' 5.39" W). The hippocampal formation limits definition was performed employing Nissl staining and immunostaining for NeuN. For the objects of interest definition of stereological estimates and three-dimensional reconstructions we used immunostaining with anti NeuN for neurons and anti-IBA-1 for microglia respectively. The stereological results revealed similar number of neurons in both species whereas the number of microglia in Actitis macularia was 37% higher than in Calidris pusilla. Furthermore, it was found that the hipocampal formation average volume in Actitis macularia was 38% higher than that found in Calidris pusilla. Comparative studies of the microglial morphology with mammals (Rattus novergicus and Cebus apella) revealed significant morphological differences that indicate microglia in birds shows on average lower complexity (smaller fractal dimension), smaller tree volumes and areas and thinner branches than rat and monkey microglia.
Acesso aberto (Open Access)
Estruturas do olho do Macrobrachium amazonicum (Heller, 1862) (Crustacea, Decapoda, Palaemonidae): estudo utilizando microscopia óptica e de varredura.
(Universidade Federal do Pará, 2011-01-19) BOULHOSA, Silvio Márcio Pereira; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
The genus Macrobrachium contains more than 120 species and occurs in tropical and subtropical regions worldwide (VALENT, 1987). They are freshwater shrimp family Palaemonidae and order Decapoda (RAFINESQUE, 1815; LATREILLE, 1802). In Brazil there are 18 species, classified until now distributed throughout the Amazon Basin (MELO 2003). Among these, Macrobrachium amazonicum (HELLER, 1862) popularly known as shrimp quiet or shrimp cinnamon, widely used in shrimp farming. Crustaceans have sensory structures located on the cephalothorax, which allow then to receive the stimulus means to locate and capture food (BARNES, 1998). The compound eyes are present in all classes of crustacean. Thus, this research hypothesis was that in Macrobrachium amazonicum, these eyes are kind of overlay reflective, where the dioptric apparatus and rhabdom extend in layers and are separated by a clear non-pigmented area. In this work we have the following objectives: To evaluate the morphological aspects of the eye of Macrobrachium amazonicum scanning electron microscopy; characterize the morphology of the photoreceptor cells; describe the morphological structures of the eye of M. amazonicum; characterize the morphometric relationships between the eye and other structures of M. amazonicum. The animals were acquired in the district of ‘Mosqueiro’ in the rainy season, in March 2009 and March 2010, with fishermen in Santa Barbara city, the metropolitan area of Belém, and transported to the laboratory in polystyrene boxes, being held in quarantine in a vessel containing a solution of potassium permanganate at 1.3 mg / L (CARNEIRO et al., 2005). The animals were fixed in Davidson Karnovisky then the eyes of each animal were carefully sectioned and placed in plastic. Relations were performed in biometric analysis of variance with α = 0.05, was performed with Bio Stats 5.0 for the lengths of the side of the eye and anterior-posterior of the four morphotypes, and SigmaPlot 11.0 and simple linear regression for the variables eye total and cephalothorax. It was observed that the average size of the cephalothorax, the body, the eye is respectively: 1.03 mm, 70.62 mm and 4.52 mm, and the average weight of shrimp was 7.97 g. The structures of these maximum values recorded were 31.95 mm for the cephalothorax; 100.10 mm body size, 6.80 mm for the size of the eye and 20.54 g for the weight of the shrimp. Histological analysis identified the following structures (Fig. 13:14 AB): cornea, crystalline cone, pigment distal shaft of the cone, clear zone, cuticle, retina, rabdoma, optic nerve fibers and laminates. With respect to scanning electron microscopy, were selected to observe the main structures and especially the arrangement of ommatidia square cross section (Figs. 16 AB). The morphological and morphometric analysis (by histology and scanning electron microscopy) showed characteristics of Macrobrachium amazonicum adults from the metropolitan region of Belém. These findings include a reflective optical superposition eyes fitted with the perception of light stimuli.
Acesso aberto (Open Access)
Estudo sobre o crescimento do tambaqui (Colossoma macropomom) submetido à dieta suplementada com camu camu (Myrciaria dubia) em água corrente e aquecida
(Universidade Federal do Pará, 2018-09) CANTO, Miguel Angelo de Oliveira; SALGADO, Claudio Guedes; http://lattes.cnpq.br/9601463988942971; DINIZ, Domingos Luiz Wanderley Picanço; http://lattes.cnpq.br/9601463988942971
This study investigated the growth of the tambaqui (Colossoma macropomum) (Cm), an caracid teleost in the Amazon region, in the laboratory. Juvenile fish were submitted to the simulation of the natural conditions of feeding, climate and running water, corresponding to the flood and dry periods of the Amazonian hydrological cycle. In the flood, its diet is predominant of fruits and seeds, the environment is mild (28 ± 2°C) and the current is higher (0.2 to 0.3 m/s). In drought, the feed is based on animal protein, there is no current and the average temperature rises (34 ± 2°C). Therefore, the aim of this study was to investigate the effects of Myrciaria dubia (Md) on the growth of Cm in the drought or flood simulations with respect to the mild (28 ° C) or heated (34 ° C), standing or running water (0,2 or 0.3m/s), and diet with higher (45%) or lower (32%) crude protein (CP) content. For this, juveniles of Cm were acclimatized (70 or 126 days) in tanks of (310 or 500 liters) according to the experimental protocol. Protocol I: diet with fractioned daily supply (3x/day) and supplemented with Md; crude protein (45 or 32%); running water (0.2 m/s) or stopped at 28 or 34°C; analysis of muscle contents of IGF1 and total lipids. protocol II: single or fractioned offer (3x / day); standing or running water (0.3 m/s), intercalated, (12 hours) or continuous; quantification of the cavity fat mass. The results are presented on average plus or minus the standard error of the mean and compared by ANOVA plus Bonferrone post-test. Correlation test for weight, length or cavity fat versus running water; in the conditions of single or fractional supply was performed to verify interrelations between the phenomena studied. A potentiation of body mass expansion rather than length occurred by the diet supplemented with Md in standing and heated water. In contrast similar potentiation occurred for running and heated water in the 45% CP diet. In turn, lower growth performance (weight and length) was observed in the group submitted to a 32% CP diet. The running water also potentiated the accumulation of cavitary and muscular fat of total lipids, suggesting that the swimming effort demands accumulation of potential energy possibly related to the preservation of protein anabolism, since the protein content in the muscle tissue was not altered. On the other hand, the single daily supply of food was not enough to maintain the resulting growth performance to sustained swimming (running water). In the fractioned supply, the group submitted to continuous running water showed the best performance, suggesting that sustained swimming in running water may be a determinant factor for the growth of Cm if it is offered a diet with high protein content, considering the warm environment as the most favorable. Finally, the increased IGF-1 content in the muscle confirms the participation of this growth factor as the final pathway of humoral regulation of muscle hypertrophy. Hypertrophy results in increased swimming effort, and, surprisingly, in response to diet supplemented by Md.
Acesso aberto (Open Access)
Filogenia do gênero Mischocyttarus de Saussurre, baseado em caracteres morfológicos e moleculares, e revisão taxonômica do subgênero Megacanthopus Ducke (Hymnoptera, Vespidae, Polistinae)
(Universidade Federal do Pará, 2019-03) FELIZARDO, Sherlem Patrícia de Seixas; SILVEIRA, Orlando Tobias; http://lattes.cnpq.br/9654506257169791; https://orcid.org/0000-0002-5899-199X
Acesso aberto (Open Access)
Fisiologia térmica e vulnerabilidade dos lagartos da Amazônia (Reptilia: Squamata) frente às mudanças climáticas
(Universidade Federal do Pará, 2017-01-12) URTIAGA, Luisa Maria Viegas Becerra; ÁVILA-PIRES, Teresa Cristina Sauer de; http://lattes.cnpq.br/1339618330655263; QUEIROZ, Helder Lima de; http://lattes.cnpq.br/3131281054700225
Is predicted that the global warming drive the Earth to an increase of temperature of almost 4.8°C by the end of the XXI century, what may negatively affects the distribution of lizards species, considering that they are ectotherms and depends directly on the environmental temperatures to regulate their body temperatures (Tb). This animals usually present as compensatory response to the thermal stress the restriction of activity time in refuges, to minimize the risk of death by overheating. It is believed that the species’ vulnerability would be inversely proportional to Tb and to the number of hours of activity restriction in refuges (Hr), and directly proportional to the maximum environmental temperature (MET), what means that both heliotherm and thermoconformer lizards should be vulnerable to the climate changes. There are little information available in literature about the biology of Amazonian lizards, although it is the most biodiverse region in the world and house about 210 species of lizards among all its extension. Therefore, the first chapter of this work had as goal to synthetize the knowledge on thermal physiology of these animals and incorporate unpublished data on thermal preferences for some species, besides to characterize their thermoregulation mode and recover the phylogenetic relationships among the characters. The second chapter, in turn, analyzes the persistence/absence status of 29 species, with projections to 2050 and 2070, in order to verify if their vulnerability is in fact associated to Tb, Hr and MET. Fourty-five studies reporting Tbs from 62 species were found, and additional data on Tb, as well as unpublished data on thermal preferences, were collected among 13 localities of Amazonia, encompassing several phytophysiognomies of the biome. Were obtained data on preferred temperature (Tpref), minimum (Vtmin) and maximum (Vtmax) voluntary temperatures and tolerance zone (critical thermals, Ctmin and Ctmax) for 33 species, and performance data (Topt) for 10 species. Tb, Tpref and Vtmin present low phylogenetic signal (K<1), and all variables are correlated, with exception of 1) Topt, which does not present correlation with Ctmax, and 2) Ctmin, which only correlates with Topt. Although shows significant statistical differences, heliotherms and thermoconformers present thermal characters intercalated in the scale of values, demonstrating a continuum between the extreme thermoregulation modes. The use of a species distribution model that mix environmental and physiological data to calculate the potential distribution of species in the present and future, considering a realistic scenario of greenhouse gases’ emissions, allows the verification of the persistence/absence status on 29 species of Amazonian lizards. Three of them, all heliotherms, presents a projection of territorial expansion by 2070. The other 26, presents a projection of reductions in their occurrence area, with heliotherms presenting lower mean values of Hr and higher mean values of Tb than thermoconformers. Has not been verified any pattern of vulnerability related to thermoregulation mode, and the majority of the species present low Tb and/or Hr, agreeing with the hypothesis that relates vulnerability to Tb, Hr and MET. Other eight species were also modeling, but they present issues in the calculation of the potential distribution and thus were not included in the vulnerability analysis. These species are being evaluated by the model’ developers to possible adjustments. Lastly, if the forecasts concretizes, the high occurrence restrictions recovered by the model can reflects a potentially high extinction risk of the species. The result is worrying, even considering the model limitations, and reinforces the need of conservation policies that take into account the effects of climate change on fauna, as well as further studies aimed to improving our understanding about the consequences of the global warming.
Acesso aberto (Open Access)
Regulação comportamental em caititus (Pecari tajacu): o efeito da estrutura social na função reprodutiva de fêmeas em cativeiro
(Universidade Federal do Pará, 2014-05-02) SILVA, Suleima do Socorro Bastos da; GUIMARÃES, Diva Anelie de Araújo; http://lattes.cnpq.br/2891287458034896
The failure in the fertility of adult female collared peccary (Pecari tajacu) bred in captivity are a restrictive factor for the efficiency of production and needs further investigation. Assess the reproductive function of females reared under intensive rearing system was the objective of this research. Twenty females were monitored in family groups (controls) for 60 days, and experimental groups (female descendants grouped with unrelated males and in the absence of parents), for more 60 days. The agonistic interactions, submission, friendly and sex were recorded three times a week, social dominance was assessed by Elo-rating method and the social preference by frequency of friendly interactions. Blood was collected for measurement of plasma progesterone by solid phase radioimmunoassay and pregnancy was confirmed by ultrasound examination. In three of the four control groups, the parents remained in the highest positions in the hierarchy while in the experimental group stage, the female descendants occupied the highest posts. The frequency of received friendly interactions depended on the sex and the condition (H = 142 991 p <0.01) whereas females mothers received, on average, 2.21 times more interactions than other individuals. Males and females received equal frequencies in the experimental groups. The estrus lasted 3.6 ± 1.0 days with progesterone at 1.2 ± 0.6 ng / mL and higher frequency of copulations in the final third of this period. Sexual interactions increased during estrus in females (P = 0.0019) and males (P <0.0009), which intensified their frequency of olfactory inspection. Copulations were recorded in the early pregnancy. All pluriparous females (heavier, older and dominant) showed reproductive activity (cyclicity or pregnancy) while seven nulliparous females had periods of anestrus, short (16 days) or long (60 days) in the control group. In the experimental group all nulliparous and primiparous mated and 14 pups were born, however, only five remained after seven days of life. We demonstrate the presence of the dominance of the parents on the progeny and a mechanism for reproductive inhibition in family groupings. We suggest that nulliparous females of reproductive age are relocated from their original group for groups with unrelated males, ensuring the reproductive potential of creation and access to food, space and other resources generally preferred prioritized by dominant individuals.
Acesso aberto (Open Access)
Taxonomia integrativa de nematódeos Oswaldocruzia (Trichostrongyloidea: Molineidae) da Amazônia Oriental
(Universidade Federal do Pará, 2019-04) COSTA, Yuri Willkens de Oliveira; SANTOS, Jeannie Nascimento dos; http://lattes.cnpq.br/4543897195525368; https://orcid.org/0000-0002-6612-6410
Oswaldocruzia is a genus of parasitic nematodes of amphibians (Anura, Caudata) and reptiles (Squamata), represented by about 90 species distributed worldwide, of which 43 occur in the Neotropical region. The species of Oswaldocruzia are mainly characterized by the spicular morphology of the males and are divided into five biogeographic groups (Oriental-Ethiopian, Neo-Ethiopian, Holartic, Neotropical Caribbean and Neotropical Continental) and also three morphological types of copulatory bursa (types I, II and III). However, morphological similarity, absence of updated identification keys and molecular data complicate the systematic of the genus. Thus, the objective of this study was the realization of the integrated taxonomy of nine species of Oswaldocruzia parasitizing eight species of amphibians and one reptile species from different collections in three locations in the state of Pará, Brazil. The hosts were necropsied and the helminths found were rinsed, fixed and stored in 70% ethanol. For the morphological study, the specimens were used for observation by light microscopy and scanning electron microscopy. For the molecular study, we performed the extraction, amplification and sequencing of the coding region of the Cytochrome c Oxidase Subunit I enzyme from the mitochondrial DNA. The resulting sequences were aligned and compared to ten sequences publicly available in GenBank and two phylogenetic reconstructions were performed to observe their relationships, one under the Maximum Likelihood criterion and the other by Bayesian Inference. As results we identified Oswaldocruzia belenensis in Rhinella marina and Rhinella margaritifera, Oswaldocruzia chabaudi in Boana geographica and Boana wavrini, Oswaldocruzia chambrieri in Amazophrynella bokermanni and R. margaritifera, Oswaldocruzia lanfrediae in Leptodactylus paraensis, Oswaldocruzia vitti in Anolis fuscuauratus, Oswaldocruzia sp. nov. 1 in Phyllomedusa vaillantii and Oswaldocruzia sp. nov. 2 in Osteocephalus oophagus. Alignment and comparison of levels of divergence demonstrated significant differences between the new obtained sequences and the sequences from GenBank. Both phylogenetic reconstructions demonstrated two main clades, one including the sequences from Mexico and another clade genetically distinct from the Amazon, highlighting the occurrence of O. chabaudi in B. wavrini and B. geographica, the close relationship between the sequences from parasites of Bufonidae and new records of hosts and localities for O. chambrieri and O. belenensis. This study adds information about the diversity of helminth parasites of amphibians and reptiles of Amazon, and shows that the combination of morphological and molecular methods presents satisfactory potential for species delimitation and characterization of the genus.