Programa de Pós-Graduação em Neurociências e Biologia Celular - PPGNBC/ICB

URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/2374

O Programa de Pós-Graduação em Neurociências e Biologia Celular (PPGNBC) é parte integrante do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA), sendo constituído por: Mestrado e Doutorado em Neurociências e Biologia Celular, com área de concentração em Neurociências ou Biologia Celular.

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Agora exibindo 1 - 6 de 6

Acesso aberto (Open Access)
Avaliação do efeito da fração lipídica extraída de Agaricus brasiliensis antioxidante e imunomoduladora in vitro e em modelo de sepse letal em murino
(Universidade Federal do Pará, 2021-01) LIMA, Kely Campos Navegantes; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390; https://orcid.org/0000-0002-3328-5650
Sepsis is defined as a potentially fatal organ dysfunction caused by a dysregulated host immune response to an infection. During sepsis, dysregulation of the host response occurs with the excessive release of pro-inflammatory mediators, generation of reactive species with depletion of antioxidant defenses and cellular damage. As a result, the patient develops organ dysfunction. In this context, our group proposes the A.brasiliensis Lipid Fraction (FLAb) as a possible therapy for sepsis considering its immunomodulatory and systemic antioxidant activity in a murine sepsis model. Thus, the present study aimed to evaluate the activity of FLAb isolated in vitro and to evaluate the effect of treatment with FLAb alone or associated with the antibiotic ertapenem (F-Erta) on coagulation, antioxidant and immunomodulatory parameters in the lethal sepsis model in murine. For this, FLAb was kindly provided by Dr. Herta Dalla- Santa from UNICENTRO. In the present study, the antioxidant capacity of different concentrations of FLAb (1.25 and 5 μg/mL) was evaluated and in a RAW 264.7-Luc macrophage cell line, cytotoxicity, phagocytic capacity, nitric oxide, NF-κB activity and cytokines TNF-α and IL-6 were evaluated.The survival rates were analyzed 7 days in a model of CLP sepsis in swiss albino mice (Mus musculus), and treated with CLP+Salt (0.9%), CLP+FLAb (0.2mg/Kg), CLP+F-erta (0.2mg) /Kg; 30mg/Kg). For evaluation of on coagulation, antioxidant and immunomodulatory parameters, the mice were treated by 6 and/or 24h after CLP. In vitro, FLAb show antioxidant and anti-inflammatory activity in both concentrations. In vivo, all CLP+Salt animals died within a maximum of 48 hours while the FLAb and F-Erta treated groups survived the 7 days. During this period, clinical parameters of these animals were evaluated, the septic animals treated with saline showed piloerection, with little active level of consciousness and most of the time they were stopped in the cage, some of them had ocular secretion. In addition, animals treated with saline showed significant weight loss, reduced water and feed consumption resulting in death. The FLAb and F-Erta groups were active, with normal appearance, with normal breathing and heart rate, in addition to consuming water and food within normal limits. In the inflammatory site, peritoneal cavity, the treatment with FLAb showed an anti-inflammatory effect, decreased reactive oxygen species (ROS) and increased GSH antioxidant activity and protected from cell damage, maintaining neutrophil recruitment and nitric oxide levels (NO), reducing the bacterial load. Regarding coagulation parameters (platelet count, tp and ttpa), treatment with FLAb and F-Erta eliminated the bacterial load and protected the animals from tissue damage. In the liver, 6 hours after CLP the treatment with FLAb and F-ERTA was observed in the biochemical parameters protective effect, in addition, it presented immunomodulatory, antimicrobial and antioxidant activity preventing liver damage. In the parameters evaluated in the heart, the treatment with FLAb and F-ERTA after CLP protected the animals from cardiac damage through immunomodulatory, antimicrobial and antioxidant activity. In this sense, FLAb alone showed promise as a treatment and/or adjunct in sepsis, in addition to preventing organic dysfunction in septic animals.
Acesso aberto (Open Access)
Dimorfismo sexual da espessura da retina: uma análise de aprendizagem de máquina
(Universidade Federal do Pará, 2022-03) FARIAS, Flavia Monteiro; SALOMÃO, Railson Cruz; http://lattes.cnpq.br/9518575270670446; SOUZA, Givago da Silva; http://lattes.cnpq.br/5705421011644718; https://orcid.org/0000-0002-4525-3971
The present research compared the accuracy of machine learning algorithms in classifying the thickness and volume measurements of retinal layers as obtained from male and female subjects. The study evaluated the retina of sixty-four healthy participants (38 women and 26 men), with normal vision and without eye or systemic diseases, aged between 20 and 40 years. The data acquisition was obtained with a Spectralis HRA+OCT tomograph in the macular region of the retina and its layers: retinal nerve fiber layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), retinal pigment epithelium (RPE), inner retina (IRL) and outer retina (ORL). The classification accuracy was obtained with the following algorithms: support vector classifier (SVC), logistic regression (LR), linear discriminant analyses (LDA), k-nearest neighbors (kNN), decision tree (DT), gaussian naive bayes (GNB) and random forest (RF). The characteristics attributed to each participant's samples were the thickness values in the nine regions of the macula plus the total macular volume of each retinal layer. The statistical tests Two-way ANOVA and Tukey HSD post-hoc were used in the statistical comparisons between the accuracies for the classifier and retinal layer variables, considering a significance level of < 0.05. All factors (classifier, retinal layer, and their interactions) had significant influences on accuracy (p < 0.05). The main effect of the algorithm type factor resulted in an F ratio of F (6, 630) = 4.527, p = 0.0002. The main effect for the retinal layer produced an F ratio of F (9, 630) = 51.64 and p < 0.0001. The interaction effect was also significant, F(54, 630) = 1.741, p = 0.0012. All algorithms classified with high accuracy (> 0.70) the innermost layers of the retina (total retina, inner retina, RNFL, GCL, INL) according to the gender of the participants, where we observed significant differences between genders in thickness and measurements volume. The SVC, LDA, and LR algorithms produced high accuracy (>0.70) when thickness and volume data came from the RNFL compared to the outermost layers of the retina. The KNN, RF and DT algorithms performed better in correctly classifying the total retina data in relation to the outermost layers. The thickness and volume of the retina and the innermost layers of the retina allow machine learning algorithms to be more accurate in separating data from different sexes.
Acesso aberto (Open Access)
Efeito do óleo da espécie vegetal Caryocar villosum em linhagem de células vero in vitro
(Universidade Federal do Pará, 2024-08) LOPES, Izabella Carla Silva; NASCIMENTO, José Luiz Martins do; http://lattes.cnpq.br/7216249286784978; https://orcid.org/0000-0003-3647-9124
Fibroblasts are cells that participate in different processes in the body, being indispensable in the development and homeostasis of tissues. They are important agents in the healing process, being present in the inflammatory, proliferative and remodeling phases. These cells produce biomolecules that constitute the extracellular matrix, such as collagen, which provide the necessary support for healthy healing. However, healing is a complex process, and it is proneto failure, so it´s important to investigate therapeutic alternatives to produce more effective treatments in cases where natural healing is not effective. Natural products from plants are widely used as therapeutic forms for healing considering their lower side effects, low cost, this combined with traditional knowledge makes it attractive to studies that prioritize valuing biodiversity. Caryocar villosum (Cv), a fruit native to the Amazon region, is a remarkable source of bioactive compounds with therapeutic potential that has cosmetic and pharmaceutical applications. In this work, the action of Cv oil (CvO) on VERO cell line (fibroblast-like cells)was investigated. The cells were treated with 75, 125 and 250 μg/mL for 24 hours. Cytotoxicity assays, optical microscopy, cell proliferation, morphological analysis, and analysis of extracellular matrix components, such as phalloidin staining and immunofluorescence for type III collagen antibody, were performed. Any cytotoxicity was observed in the cells after CvOtreatment. CvO treatment induced morphological and physiological changes in the cells indicating cell activation. Fluorescence microscopy revealed greater cell spreading, rearrangement of actin filaments and increased cell surface exposure compared to untreated cells. Furthermore, this study suggests that fibroblasts treated with CvO are activated through cytoskeletal rearrangement, increasing cell surface, proliferating, and increasing collagen synthesis. These findings indicate that CvO may be a good therapeutic strategy from biodiversity to stimulate the healing process.
Acesso aberto (Open Access)
Efeitos da suplementação com açaí clarificado (Euterpe oleracea Mart.) sobre marcadores de estresse oxidativo em pacientes com doença renal crônica em hemodiálise
(Universidade Federal do Pará, 2019-06) MARTINS, Isabelle Christine Vieira da Silva; NASCIMENTO, José Luiz Martins do; http://lattes.cnpq.br/7216249286784978; https://orcid.org/0000-0003-3647-9124
Acesso aberto (Open Access)
Estudo sobre o crescimento do tambaqui (Colossoma macropomom) submetido à dieta suplementada com camu camu (Myrciaria dubia) em água corrente e aquecida
(Universidade Federal do Pará, 2018-09) CANTO, Miguel Angelo de Oliveira; SALGADO, Claudio Guedes; http://lattes.cnpq.br/9601463988942971; DINIZ, Domingos Luiz Wanderley Picanço; http://lattes.cnpq.br/9601463988942971
This study investigated the growth of the tambaqui (Colossoma macropomum) (Cm), an caracid teleost in the Amazon region, in the laboratory. Juvenile fish were submitted to the simulation of the natural conditions of feeding, climate and running water, corresponding to the flood and dry periods of the Amazonian hydrological cycle. In the flood, its diet is predominant of fruits and seeds, the environment is mild (28 ± 2°C) and the current is higher (0.2 to 0.3 m/s). In drought, the feed is based on animal protein, there is no current and the average temperature rises (34 ± 2°C). Therefore, the aim of this study was to investigate the effects of Myrciaria dubia (Md) on the growth of Cm in the drought or flood simulations with respect to the mild (28 ° C) or heated (34 ° C), standing or running water (0,2 or 0.3m/s), and diet with higher (45%) or lower (32%) crude protein (CP) content. For this, juveniles of Cm were acclimatized (70 or 126 days) in tanks of (310 or 500 liters) according to the experimental protocol. Protocol I: diet with fractioned daily supply (3x/day) and supplemented with Md; crude protein (45 or 32%); running water (0.2 m/s) or stopped at 28 or 34°C; analysis of muscle contents of IGF1 and total lipids. protocol II: single or fractioned offer (3x / day); standing or running water (0.3 m/s), intercalated, (12 hours) or continuous; quantification of the cavity fat mass. The results are presented on average plus or minus the standard error of the mean and compared by ANOVA plus Bonferrone post-test. Correlation test for weight, length or cavity fat versus running water; in the conditions of single or fractional supply was performed to verify interrelations between the phenomena studied. A potentiation of body mass expansion rather than length occurred by the diet supplemented with Md in standing and heated water. In contrast similar potentiation occurred for running and heated water in the 45% CP diet. In turn, lower growth performance (weight and length) was observed in the group submitted to a 32% CP diet. The running water also potentiated the accumulation of cavitary and muscular fat of total lipids, suggesting that the swimming effort demands accumulation of potential energy possibly related to the preservation of protein anabolism, since the protein content in the muscle tissue was not altered. On the other hand, the single daily supply of food was not enough to maintain the resulting growth performance to sustained swimming (running water). In the fractioned supply, the group submitted to continuous running water showed the best performance, suggesting that sustained swimming in running water may be a determinant factor for the growth of Cm if it is offered a diet with high protein content, considering the warm environment as the most favorable. Finally, the increased IGF-1 content in the muscle confirms the participation of this growth factor as the final pathway of humoral regulation of muscle hypertrophy. Hypertrophy results in increased swimming effort, and, surprisingly, in response to diet supplemented by Md.
Acesso aberto (Open Access)
Estudos citogenéticos e moleculares nos gêneros Mesomys e Lonchothrix (Rodentia, Echimyidae, Eumysopinae)
(Universidade Federal do Pará, 2022-08-29) OLIVEIRA, Leony Dias de; SILVA, Willam Oliveira da; http://lattes.cnpq.br/0903402972891613; https://orcid.org/0000-0003-3125-1075; NAGAMACHI, Cleusa Yoshiko; http://lattes.cnpq.br/8887641213110093; https://orcid.org/0000-0003-1516-2734
The Echimyidae family is considered the most taxonomically diverse among South American hystricognathi rodents, comprising 25 genera and 93 species. The subfamily Eumysopinae is represented by nine genera, among which we highlight the arboreal genera Mesomys, which has four recognized species, and Lonchothrix described as monotypic (L. emiliae), both distributed in the Amazon. Morphological, molecular and chromosomal studies in the genera Mesomys and Lonchothrix have contributed to a better understanding of taxonomic design, phylogenetic relationships and karyotypic patterns. Recent molecular investigations have shown an as yet undescribed diversity, suggesting that these taxa are even more diverse than previously assumed. Furthermore, the limits of geographic distribution in the Amazon for the species M. hispidus and M. stimulax have been questioned by some authors. In this sense, the current study sought to investigate the karyotypic diversity and the geographic limits of the Mesomys and Lonchothrix genera, based on classical and molecular cytogenetic analysis and through sequences of the mitochondrial genes Cytochrome b (Cytb) and Cytochrome Oxidase - Subunit I (COI) from different locations in the Brazilian Amazon. The species M. stimulax and Mesomys sp. n. presented 2n=60/NF=110, while M. hispidus presented 2n=60/NF=112 and Lonchothrix emiliae presented 2n=66/NF=126, both unpublished karyotypes for the genera. Phylogenetic analysis confirmed Mesomys and Lonchothrix as sister genera and showed a high intraspecific rate in M. hispidus and Mesomys sp. n. from Itaituba, may be related to a new lineage in the genus Mesomys.

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