Artigos Científicos - ICB

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Acesso aberto (Open Access)
Amphibians and reptiles from Floresta Nacional de Pau-Rosa, Amazonas, Brazil: an important protected area at the heart of Amazonia
(Instituto Nacional de Pesquisas da Amazônia, 2017-09) FERREIRA, Gisele Cassundé; STURARO, Marcelo José; PELOSO, Pedro Luiz Vieira Del
Amazonia harbors the largest and most diverse tropical forest in the world, but knowledge about the species diversity of the region is still far from ideal. Given this low level of faunal and floral knowledge, we present an annotated list of the species of amphibians and reptiles found in Floresta Nacional do Pau-Rosa (FNPR), along the Rio Paraconi, municipality of Maués, state of Amazonas, Brazil. Herpetofauna of the FNPR was sampled with pitfall traps, active search and occasional encounters from February 18 to March 28, 2009. A total of 270 specimens were collected, representing 39 species of amphibians and 24 species of reptiles. At least seven of the species collected at FNPR represented, at the time, unnamed taxa (four of which have now been named). The number of taxa collected and the high number of unnamed taxa highlight the importance of this area in terms of biodiversity and as a priority for conservation. We also discuss about the amphibian diversity in Amazonia.
Acesso aberto (Open Access)
Biometric relationships between body size and otolith size in 15 demersal marine fish species from the northern Brazilian coast
(Instituto Nacional de Pesquisas da Amazônia, 2019-12) OLIVEIRA, Rory Romero de Sena; ANDRADE, Marcelo Costa; MACHADO, Fabiola Seabra; CUNHA, Élida Jesana Santana; FREITAS, Flaviane Souto de; KLAUTAU, Alex Garcia Cavalleiro de Macedo; GIARRIZZO, Tommaso; SAINT-PAUL, Ulrich
The relationships between fish size and sagitta otolith measurements were calculated for the first time for 15 species belonging to six families from the northern Brazilian coast. A total of 220 fish were sampled from the bycatch landed by the bottom-trawl industrial shrimp-fishing fleet between August and September 2016. All species had strong relationships between otolith measurements and fish total length with the coefficient of determination (r 2) ranging between 0.71 and 0.99. The variable most strongly related to fish total length was found to be the sagittal otolith length (OL) with 98% of the variability. These relationships are a useful tool to estimate length and mass of preyed fish from otoliths found in stomach contents of marine predators.
Acesso aberto (Open Access)
Caracterização farmacognóstica de Piper arboreum var. Arboreum e P. tuberculatum (Piperaceae)
(Instituto Nacional de Pesquisas da Amazônia, 2016-06) SILVA, Rolf Junior Ferreira; DIAS, Ana Cristina Andrade de Aguiar; FAIAL, Kelson do Carmo Freitas; MENDONÇA, Maria Sílvia de
Piper arboreum Aubl. var. arboreum and P. tuberculatum Jacq. (Piperaceae), both known as “pimenta-longa”, are frequently misidentified. Despite the fact that both species are sold as traditional phytotherapeutic products, there are no reports on their quality control. The aim of this study was to prepare and characterize leaf and stem herbal drugs of both species using standard methods aiming to establish parameters for botanical authentication and purity degree for their quality control. Size, shape, color, taste, texture, fracture surface and transection characteristics, and leaf venation patterns are macroscopic diagnostic characters useful to distinguish the herbal drugs when they are not powdered. The following characters are useful for diagnostic purposes of the powdered herbal drugs: cuticular and epicuticular wax patterns, types of trichomes, and wall thickening of the tracheary elements, and shape of the hilum of the starch grains. Emergences are key morphological markers for P. tuberculatum herbal drugs. Physicochemical parameter values were similar in herbal drugs of both species, except acid-insoluble ash value which was higher in the P. arboreum var. arboreum stem herbal drugs. Piper arboreum var. arboreum herbal drugs had the highest metal concentrations. Al, Cu, Mn and V concentrations were the highest, whereas Cd, Co and Hg concentrations were the lowest in the herbal drugs of both species. The results obtained can be used as quality control parameters for the herbal drugs of both species.
Acesso aberto (Open Access)
Complete genome sequence of Corynebacterium pseudotuberculosis biovar ovis strain P54B96 isolated from antelope in South Africa obtained by rapid next generation sequencing technology
(BioMed Central, 2012) HASSAN, Syed Shah; GUIMARÃES, Luís Carlos; PEREIRA, Ulisses de Pádua; ISLAM, Arshad; ALI, Amjad; BAKHTIAR, Syeda Marriam; RIBEIRO, Dayana; SANTOS, Anderson Rodrigues dos; SOARES, Siomar de Castro; DORELLA, Fernanda Alves; GOMIDE, Anne Cybelle Pinto; SCHNEIDER, Maria Paula Cruz; BARBOSA, Maria Silvanira Ribeiro; ALMEIDA, Sintia Silva de; ABREU, Vinicius Augusto Carvalho de; ABURJAILE, Flavia Figueira; CARNEIRO, Adriana Ribeiro; CERDEIRA, Louise Teixeira; NASCIMENTO, Karina Kelly Fiaux do; BARBOSA, Eudes Guilherme Vieira; DINIZ, Carlos Augusto Almeida; ROCHA, Flávia de Souza; RAMOS, Rommel Thiago Juca; JAIN, Neha; TIWARI, Sandeep; BARH, Debmalya; MIYOSHI, Anderson; MÜLLER, Borna; SILVA, Artur Luiz da Costa da; AZEVEDO, Vasco Ariston de Carvalho
The Actinobacteria, Corynebacterium pseudotuberculosis strain P54B96, a nonmotile, non sporulating and a mesophile bacterium, was isolated from liver, lung and mediastinal lymph node lesions in an antelope from South Africa. This strain is interesting in the sense that it has been found together with non-tuberculous mycobacteria (NTMs) which could nevertheless play a role in the lesion formation. In this work, we describe a set of features of C. pseudotuberculosis P54B96, together with the details of the complete genome sequence and annotation. The genome comprises of 2.34 Mbp long, single circular genome with 2,084 protein-coding genes, 12 rRNA, 49 tRNA and 62 pseudogenes and a G+C content of 52.19%. The analysis of the genome se quence provides means to better understanding the molecular and genetic basis of virulence of this bacterium, enabling a detailed investigation of its pathogenesis.
Acesso aberto (Open Access)
Complete genome sequence of Corynebacterium pseudotuberculosis I19, a strain isolated from a cow in Israel with bovine mastitis
(American Society for Microbiology, 2011-01) SILVA, Artur Luiz da Costa da; SCHNEIDER, Maria Paula Cruz; CERDEIRA, Louise Teixeira; BARBOSA, Maria Silvanira Ribeiro; RAMOS, Rommel Thiago Juca; CARNEIRO, Adriana Ribeiro; OLIVEIRA, Rodrigo Santos de; LIMA, Margarida Maria Celeira de; CONCEIÇÃO, Marília Lima da; FERREIRA, Vívian D'Afonseca da Silva; ALMEIDA, Sintia Silva de; SANTOS, Anderson Rodrigues dos; SOARES, Siomar de Castro; GOMIDE, Anne Cybelle Pinto; ALI, Amjad; DORELLA, Fernanda Alves; ROCHA, Flávia de Souza; ABREU, Vinicius Augusto Carvalho de; TROST, Eva; TAUCH, Andreas; SHPIGEL, Nahum; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho
This work reports the completion and annotation of the genome sequence of Corynebacterium pseudotuberculosis I19, isolated from an Israeli dairy cow with severe clinical mastitis. To present the whole-genome sequence, a de novo assembly approach using 33 million short (25-bp) mate-paired SOLiD reads only was applied. Furthermore, the automatic, functional, and manual annotations were attained with the use of several algorithms in a multistep process.
Acesso aberto (Open Access)
Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain Cp267, Isolated from a Llama
(American Society for Microbiology, 2012-07) LOPES, Thiago Souza; SILVA, Artur Luiz da Costa da; RAMOS, Rommel Thiago Juca; CARNEIRO, Adriana Ribeiro; DORELLA, Fernanda Alves; ROCHA, Flávia de Souza; SANTOS, Anderson Rodrigues dos; LIMA, Alex Ranieri Jerônimo; GUIMARÃES, Luís Carlos; BARBOSA, Eudes Guilherme Vieira; RIBEIRO, Dayana; NASCIMENTO, Karina Kelly Fiaux do; DINIZ, Carlos Augusto Almeida; ABREU, Vinicius Augusto Carvalho de; ALMEIDA, Sintia Silva de; HASSAN, Syed Shah; ALI, Amjad; BAKHTIAR, Syeda Marriam; ABURJAILE, Flavia Figueira; GOMIDE, Anne Cybelle Pinto; SOARES, Siomar de Castro; PEREIRA, Ulisses de Pádua; SCHNEIDER, Maria Paula Cruz; MIYOSHI, Anderson; EDMAN, Judy; SPIER, Sharon; AZEVEDO, Vasco Ariston de Carvalho
In this work we report the genome of Corynebacterium pseudotuberculosis strain 267, isolated from a llama. This pathogen is of great veterinary and economic importance, as it is the cause of caseous lymphadenitis in several livestock species around the world and causes significant losses due to the high cost of treatment.
Acesso aberto (Open Access)
Complete Genome Sequence of Type Strain Campylobacter fetus subsp. venerealis NCTC 10354
(American Society for Microbiology, 2011-10) STYNEN, Ana Paula Reinato; LAGE, Andrey Pereira; MOORE, Robert J.; REZENDE, Antonio Mauro; FERREIRA, Vívian D'Afonseca da Silva; RUY, Patrícia de Cássia; OLIVEIRA, Nesley Jesus Daher de; RESENDE, Daniela de Melo; ALMEIDA, Sintia Silva de; SOARES, Siomar de Castro; ABREU, Vinicius Augusto Carvalho de; ROCHA, Aryane Aparecida Magalhães Cassiano; SANTOS, Anderson Rodrigues dos; BARBOSA, Eudes Guilherme Vieira; COUTO, Danielle Costa Carrara; DORELLA, Fernanda Alves; MIYOSHI, Anderson; LIMA, Alex Ranieri Jerônimo; CAMPOS, Frederico Davi da Silva; SÁ, Pablo Henrique Caracciolo Gomes de; LOPES, Thiago Souza; RODRIGUES, Ryan Maurício Araújo; CARNEIRO, Adriana Ribeiro; LEÃO, Tiago Ferreira; CERDEIRA, Louise Teixeira; RAMOS, Rommel Thiago Juca; SILVA, Artur Luiz da Costa da; AZEVEDO, Vasco Ariston de Carvalho; RUIZ, Jeronimo Conceição
Campylobacter fetus subsp. venerealis is the etiologic agent of bovine genital campylobacteriosis, a sexually transmitted disease of cattle that is of worldwide importance. The complete sequencing and annotation of the genome of the type strain C. fetus subsp. venerealis NCTC 10354T are reported.
Acesso aberto (Open Access)
Copaiba oil effect under different pathways in mice subjected to sepsis
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-08) BOTELHO, Nara Macedo; SILVEIRA, Edvaldo Lima; LOPES, Letícia Nobre; SANTOS, Felipe Augusto Folha; TEIXEIRA, Renan Kleber Costa; SILVA, Thaís Travassos da
PURPOSE: To evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture. METHODS: Thirty two mice were distributed into four study groups (N=8): Sham group: normal standard animals; Control group: submitted a cecal ligation and puncture (CLP); Gavage group: submitted a CLP, and treat with copaiba oil by gavage; and Subcutaneous group: submitted a CLP, and treat with copaiba oil by subcutaneous injection. After the death of the histological analysis were performed. The Kaplan-Meier curves of surviving time were realized. RESULTS: All animals that received copaiba, regardless of the route used, survived longer when compared to the control group (p<0.0001), whereas the survival time ranged from 20 hours for the control group up to 32 hours for the animals of gavage group and 52 for subcutaneous group. The animals that received gavage copaiba lived about and about 20 hours unless the subcutaneous group (p=0.0042). There was no statistical difference when compared the intensity of inflammatory response (p>0.05). CONCLUSION: Prophylactic subcutaneous administration of copaiba in mice subjected to severe sepsis by cecal ligation and puncture, resulted in a survival time higher than non-use or use of this oil by gavage.
Acesso aberto (Open Access)
Dislipidemia em escolares na rede privada de Belém
(Sociedade Brasileira de Cardiologia, 2009-06) RIBAS, Simone Augusta; SILVA, Luiz Carlos Santana da
Background: Currently, childhood dyslipidemia, associated to other non-transmissible diseases such as diabetes, hypertension and obesity, represent a significant public health problem in Brazil. Objective: To investigate the prevalence of dyslipidemia in children and adolescents from private schools in the city of Belem, state of Para, Brazil. Methods: Transversal and prospective study that assessed 437 schoolchildren, paired by sex. The age range was established between 6 and 19 years of age and stratified in four subgroups (6 to 9 years; 10 to 12 years; 13 to 15 years and 16 to 19 years). To obtain the anthropometric variables, weight and height were measured for the calculation of the body mass index and skin folds were measured for the calculation of body fat percentage. The serum lipoprotein profile was obtained through the measurement of total cholesterol, triglycerides, LDL-cholesterol and HDL-cholesterol after a 12-hour fasting period, by enzymatic methods. Results: Of the total number of schoolchildren analyzed, 126 (28.8%) were overweight and 158 (36.2%) presented a high adiposity index. The children (33.6%) presented a higher prevalence of obesity when compared to the adolescents (10.1%; p < 0.001). Regarding the biochemical characteristics, it was observed that 214 (41%) presented some alteration in the lipid profile and that children and adolescents in the age range of 10 to 15 years were the age groups that presented the highest rates of dyslipidemia (34.6% and 25.5%), respectively. Conclusion: These findings demonstrate the importance of establishing an early diagnosis of the lipid profile, mainly if it is already associated to another risk factor, such as obesity. (Arq Bras Cardiol 2009; 92(6) : 412-417).
Acesso aberto (Open Access)
Draft genome sequences of two species of “difficult-to-identify” human-pathogenic corynebacteria: implications for better identification tests.
(Ivyspring International Publisher, 2015) PACHECO, Luis Gustavo Carvalho; GUARALDI, Ana Luiza de Mattos; SANTOS, Carolina Silva; VERAS, Adonney Allan de Oliveira; GUIMARÃES, Luís Carlos; ABREU, Vinicius Augusto Carvalho de; PEREIRA, Felipe Luiz; SOARES, Siomar de Castro; DORELLA, Fernanda Alves; CARVALHO, Alex Fiorini de; LEAL, Carlos Augusto Gomes; FIGUEIREDO, Henrique Cesar Pereira; RAMOS, Juliana Nunes; VIEIRA, Verônica Viana; FARFOUR, Eric; GUISO, Nicole; HIRATA JÚNIOR, Raphael; AZEVEDO, Vasco Ariston de Carvalho; SILVA, Artur Luiz da Costa da; RAMOS, Rommel Thiago Juca
Non-diphtheriae Corynebacterium species have been increasingly recognized as the causative agents of infections in humans. Differential identification of these bacteria in the clinical microbiology laboratory by the most commonly used biochemical tests is challenging, and normally requires additional molecular methods. Herein, we present the annotated draft genome sequences of two isolates of “difficult-to-identify” human-pathogenic corynebacterial species: C. xerosis and C. minutissimum. The genome sequences of ca. 2.7 Mbp, with a mean number of 2,580 protein en coding genes, were also compared with the publicly available genome sequences of strains of C. amycolatum and C. striatum. These results will aid the exploration of novel biochemical reactions to improve existing identification tests as well as the development of more accurate molecular identification methods through detection of species-specific target genes for isolate’s identification or drug susceptibility profiling.
Acesso aberto (Open Access)
Ecological studies of mayflies (Insecta, Ephemeroptera): can sampling effort be reduced without losing essential taxonomic and ecological information?
(Instituto Nacional de Pesquisas da Amazônia, 2018-06) SHIMANO, Yulie; MATOS, Mylena Neves Cardoso; JUEN, Leandro
The present study evaluated the potential for the reduction of sampling effort in studies of ephemeropteran nymphs in Brazilian Amazon streams, Pará State, Brazil, without the loss of ecological information (species composition, abundance, and richness), and the congruence of different levels of taxonomic resolution (morphospecies, genus, family and functional group). Test groups of 15, 10 and five subsamples were selected from the 20 subsamples collected per stream (40 streams sampled), and were compared in terms of their species richness and abundance (ANOVA), and composition (Procrustes). Taxonomic resolution was also analyzed in Procrustes. Species abundance (F(3, 156) = 25.426; p < 0.001) and richness (F(3, 156) = 13.866, p < 0.001) varied significantly among sample groups, while the results of the 15-S group were statistically similar, in both cases, to those of the 20-S group. A similar pattern was found for species composition. The genus-level taxonomic resolution produced results 99% similar to those found for the species-level data. The results indicate that the reduction in sampling effort from 20 to 15 subsamples per site and a genus-level taxonomic resolution would not affect the reliability of analyses significantly. A reduction of five samples per site would result in a decrease of effort in the field and the amount of material to be processed, reducing laboratory time. In addition to a reduction in the time and resources needed to identify specimens, the adoption of a genus-level taxonomic resolution could help minimize errors of under- or over-estimation in the processing of the results.
Acesso aberto (Open Access)
Environmental structure affects taxonomic diversity but not functional structure of understory birds in the southwestern Brazilian Amazon
(Instituto Nacional de Pesquisas da Amazônia, 2019-09) OLIVEIRA, Jocieli de; ALMEIDA, Sara Miranda; FLORÊNCIO, Fernando do Prado; PINHO, João Batista de; OLIVEIRA, Dalci Mauricio Miranda de; SANTOS, Raphael Ligeiro Barroso; RODRIGUES, Domingos de Jesus
Changes in environmental characteristics can affect habitat use by birds, impacting the number of individuals, number of species, and changing species composition and functional structure of assemblages. Metrics that evaluate the functional structure of biological assemblages constitute a complementary tool to the traditional taxonomic approach, because they quantify the differences between species by means of functional traits. We assessed the effect of environmental characteristics on the taxonomic diversity (species richness, species composition and number of individuals) and functional structure (functional richness, functional evenness, Rao’s functional diversity, and community-weighted mean traits) of bird assemblages in northern Mato Grosso state, in the southwestern Brazilian Amazon. We sampled birds in 32 plots. At each plot birds were captured using mist nets, and eight environmental variables were measured: canopy openness, leaf litter, elevation, number of trees in three classes of DBH, soil clay content, and distance to nearest stream. To evaluate functional structure, we measured seven morphological traits from individuals of each bird species. Habitat variables had a significant effect on taxonomic diversity. However, the general functional structure was not affected. Elevation and distance to nearest stream were the main variables driving changes in taxonomic diversity and had a minor effect on functional richness. The other metrics of functional structure were not significantly affected by the set of environmental variables. Our results suggest that the sampled bird assemblages exhibit some functional tolerance (redundancy) to small-scale environmental variation, implying certain resilience to ecosystem modification.
Acesso aberto (Open Access)
Fatores de risco cardiovascular e fatores associados em escolares do Município de Belém, Pará, Brasil
(Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz, 2014-03) RIBAS, Simone Augusta; SILVA, Luiz Carlos Santana da
This cross-sectional study aimed to identify risk factors for cardiovascular disease in a stratified cluster sample of 557 schoolchildren (6-19 years) in Belém, Pará State, Brazil. Potential risk factors were obesity, hypertension, dyslipidemia, diabetes, smoking, physical inactivity, and atherogenic diet. Socio-demographic and lifestyle variables were tested in a binary logistic regression model. The most prevalent risk factors were overweight (20.4%), dyslipidemia (48.1%), and physical inactivity (66.2%). Children below ten years of age and those from higher-income families and with higher maternal schooling showed greater odds of developing overweight; meanwhile, those with overweight were more prone to developing hypercholesterolemia and hypertriglyceridemia. The findings point to the need to implement strategies to prevent overweight in early childhood, through balanced nutrition and regular physical activity, in order to effectively reduce the prevalence of risk factors in schoolchildren.
Acesso aberto (Open Access)
Feeding ecology of immature Lithodoras dorsalis (Valenciennes, 1840) (Siluriformes: Doradidae) in a tidal environment, estuary of the rio Amazonas
(Sociedade Brasileira de Ictiologia, 2015-06) BARBOSA, Thiago Augusto Pedroso; BARTHEM, Ronaldo Borges; MONTAG, Luciano Fogaça de Assis
Studies of feeding ecology are important for the evaluation of interactive processes in fish communities. This study evaluated the feeding ecology of Lithodoras dorsalis (Doradidae) from streams within the Amazon estuary delta (Brazil), a macro-tidal area, on different pluviometric periods. A total of 371 young specimens was collected during 12 months of sampling (July 2010 to June 2011). The species diet was composed of 28 food items analyzed by Repletion Index, Alimentary Index and Niche Breadth. Young L. dorsalis was classified as herbivore with a frugivory tendency due to the high importance of fruit and seeds in its diet. Food intake varied among sampled months, with the lowest intake being recorded during the rainy-dry season transition period, and the highest at the beginning of the dry season. The importance of food items and the composition of the diet were different throughout the year, probably due to the daily tides that allow fish to access new environments and the pluviometric periods. These results provide important data on the feeding ecology of Amazonian doradids. The study also emphasized the importance of allochthonous resources, derived from the riparian forest, which reinforces the importance of this habitat for the conservation of Neotropical freshwater fishes.
Acesso aberto (Open Access)
Feeding ecology of Serrasalmus gouldingi (Characiformes: Serrasalmidae) in the lower Anapu River region, Eastern Amazon, Brazil
(Instituto Nacional de Pesquisas da Amazônia, 2016-09) PRUDENTE, Bruno da Silveira; MARINHO, Pedro Carneiro; VALENTE, Roberta de Melo
Studies on feeding ecology of fishes are important to understand the relationship between species and environmental seasonal variations. In tropical rivers, these relationships are mainly modeled by hydrological patterns. Thereby, this study aimed to assess the influence of fluviometric variation and life stage (juveniles and adults) in the feeding ecology of Serrasalmus gouldingi in the lower Anapu River region, located in Eastern Amazon, Pará, Brazil. Specimens were collected bimonthly, considering four different hydrological periods. We assessed the diet composition, feeding intensity and niche breadth of the species. Thirty-two dietary items were identified and grouped into ten categories. A total of 279 stomachs were analyzed, showing a predominance of fish fragments, followed by fruits and seeds. The diet composition of S. gouldingi differed only between drought and flood season, although it did not differ between juveniles and adults. An increase in feeding intensity was recorded during the rise in the water level, with a lower feeding intensity observed during transitional season. Serrasalmus gouldingi showed lower niche breadth during flood season, attributed to the high consumption of fruits and seeds, presenting an omnivorous diet with high tendency towards piscivory. Although less evident than in other Amazon watersheds, the flood pulse in the lower Anapu River region is an important factor influencing the feeding ecology of the species.
Acesso aberto (Open Access)
Identification of duplicates of cassava accessions sampled on the North Region of Brazil using microsatellite markers
(Instituto Nacional de Pesquisas da Amazônia, 2013-12) MOURA, Elisa Ferreira; FARIAS NETO, João Tomé de; SAMPAIO, José Edson; SILVA, Diehgo Tuloza da; RAMALHO, Girena Fernandes
Duplicates are common in germplasm banks and their identification is needed to facilitate germplasm bank management and to reduce maintenance costs. The aim of this work was to identify duplicates of cassava from a germplasm bank in Eastern Amazon, which had been previously characterized both morphological and agronomically. In order to be genotyped with 15 microsatellite loci, 36 accessions were selected. These accessions were classified into 13 groups of similar morpho-agronomical characteristics. All loci were polymorphic, and 75 alleles were identified, with an average of five alleles per loci and HE = 0.66. There were determined 34 pairs of genotypes with identical multiloci profiles and the probability of genetic identity was 1.1x10-12 with probability of exclusion of 99.9999%. Among these duplicates, there are accessions sampled on different years and places, but with different names and accessions with the same name sampled in different places and years. The study identified genotypes that are grown in different places and that have been maintained over the years by local farmers.
Acesso aberto (Open Access)
JC virus/human immunodeficiency virus 1 co-infection in the Brazilian Amazonian region
(Sociedade Brasileira de Infectologia, 2016-08) VALLINOTO, Izaura Maria Vieira Cayres; VALLINOTO, Antonio Carlos Rosário; PENA, Giselle Priscila dos Anjos; AZEVEDO, Vânia Nakauth; MACHADO, Luiz Fernando Almeida; ISHAK, Marluísa de Oliveira Guimarães; ISHAK, Ricardo
JC virus (JCV) is a member of the Polyomaviridae family and is associated with a severe disease known as progressive multifocal leukoencephalopathy, PML, which is progressively increasing in incidence as an opportunistic infection among AIDS patients. The present study aimed to investigate the occurrence of JCV among HIV-1 carriers including their types and molecular subtypes and the possible association with disease.Urine samples from 66 HIV-1 infected subjects were investigated for the presence of the virus by amplifying VP1 (215 bp) and IG (610 bp) regions using the polymerase chain reaction. JCV was detected in 32% of the samples. The results confirmed the occurrence of type B (subtype Af2); in addition, another polyomavirus, BKV, was also detected in 1.5% of samples of the HIV-1 infected subjects. Apparently, there was no significant difference between mono- (HIV-1 only) and co-infected (HIV-1 / JCV) subjects regarding their TCD4 + / TCD8 + lymphocyte counts or HIV-1 viral plasma load. Self admitted seizures, hearing and visual loses were not significantly different between the two groups.
Acesso aberto (Open Access)
Molecular characterization of 'sweet' cassavas (Manihot esculenta) from a germplasm bank in Brazilian Eastern Amazonia
(Sociedade Brasileira de Melhoramento de Plantas, 2016-03) CUNHA, Elisa Ferreira Moura; SILVA, Carlos Rogério de Sousa; ALBUQUERQUE, Paulo Sérgio Bevilaqua de; RAMALHO, Girena Fernandes; PONTES, Lígia Cristine Gonçalves; FARIAS NETO, João Tomé de
Genetic variability of a set of 81 accessions of 'sweet' cassavas (Manihot esculenta) collected mostly in the North region of Brazil was investigated with nine microsatellite loci. All loci were polymorphic, with mean of 6.33 alleles per locus. Analyses indicated that 35 multiloci profiles were represented by a single accession, and 46 showed non-unique profiles, represented by eleven genotypes. Forty-six different multiloci profiles were detected. Most of the putative duplicated accessions were collected in different locations. After the removal of putative duplicated genotypes, genetic parameters were estimated and expected heterozygosity was high (HE=0.73), indicating genetic variability. Structure analysis of this set of 'sweet' cassavas divided the 46 genotypes into two clusters (K=2), and a few genotypes had mixed ancestry. Results indicated the habit of exchange of materials among farmers of the North region of Brazil, and the genetic variability to be exploited in genetic breeding efforts.
Acesso aberto (Open Access)
Mycological diversity description II
(Sociedade Botânica do Brasil, 2019-02-04) SILVA, Rejane Maria Ferreira da; SOARES, Adriene Mayra; PÁDUA, Ana Patrícia Sousa Lopes de; FIRMINO, André Luiz; MOTTA, Cristina Maria de Souza; SILVA, Gladstone Alves da; PLAUTZ JUNIOR, Helio Longoni; BEZERRA, Jadson Diogo Pereira; PAIVA, Laura Mesquita; OLIANI, Luisa Cunha; MÉLO, Mayara Alice Correia de; MAGALHÃES, Oliane Maria Correia; PEREIRA, Olinto Liparini; OLIVEIRA, Rafael José Vilela de; GIBERTONI, Tatiana Baptista; OLIVEIRA, Thays Gabrielle Lins de; SVEDESE, Virginia Michelle; FAN, Xinlei; RYVARDEN, Leif
Here, Diaporthe myracrodruonis is introduced as new species from Brazil, isolated as endophyte from Myracrodruon urundeuva. Asterina mandaquiensis is epitypified and ilustrated for the first time. Serpula similis is reported as new to the Neotropics, while Perenniporia centrali-africana is reported for the first time as endophyte and Preussia africana as endophyte from Spondias tuberosa in Caatinga in Brazil.
Acesso aberto (Open Access)
PIPS: Pathogenicity Island Prediction Software
(Public Library of Science, 2012-02) SOARES, Siomar de Castro; ABREU, Vinicius Augusto Carvalho de; RAMOS, Rommel Thiago Juca; CERDEIRA, Louise Teixeira; SILVA, Artur Luiz da Costa da; BAUMBACH, Jan; TROST, Eva; TAUCH, Andreas; HIRATA JÚNIOR, Raphael; GUARALDI, Ana Luiza de Mattos; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho
The adaptability of pathogenic bacteria to hosts is influenced by the genomic plasticity of the bacteria, which can be increased by such mechanisms as horizontal gene transfer. Pathogenicity islands play a major role in this type of gene transfer because they are large, horizontally acquired regions that harbor clusters of virulence genes that mediate the adhesion, colonization, invasion, immune system evasion, and toxigenic properties of the acceptor organism. Currently, pathogenicity islands are mainly identified in silico based on various characteristic features: (1) deviations in codon usage, G+C content or dinucleotide frequency and (2) insertion sequences and/or tRNA genetic flanking regions together with transposase coding genes. Several computational techniques for identifying pathogenicity islands exist. However, most of these techniques are only directed at the detection of horizontally transferred genes and/or the absence of certain genomic regions of the pathogenic bacterium in closely related non-pathogenic species. Here, we present a novel software suite designed for the prediction of pathogenicity islands (pathogenicity island prediction software, or PIPS). In contrast to other existing tools, our approach is capable of utilizing multiple features for pathogenicity island detection in an integrative manner. We show that PIPS provides better accuracy than other available software packages. As an example, we used PIPS to study the veterinary pathogen Corynebacterium pseudotuberculosis, in which we identified seven putative pathogenicity islands.