Dissertações em Farmacologia e Bioquímica (Mestrado) - FARMABIO/ICB
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/13299
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Item Acesso aberto (Open Access) Efeito da temperatura e molaridade na avaliação das atividades antimicrobiana, citotóxica e antioxidante do bio-óleo da semente do açaí (Euterpe oleracea, Mart.)(Universidade Federal do Pará, 2024-08) SILVA, Iago Castro da; MACHADO, Nélio Teixeira; http://lattes.cnpq.br/5698208558551065; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390; https://orcid.org/0000-0002-3328-5650Açaí, a fruit from the Amazon, is valuable both economically and nutritionally. Its seeds, which are typically discarded, can be converted into bio-oil through pyrolysis (a process of thermochemical degradation of residual biomass), offering a sustainable alternative to fossil fuels. This study explores how temperature and molarity with Potassium Hydroxide (KOH) and Hydrochloric Acid (HCl), which are chemical impregnation reagents in the process, affect the antimicrobial, antioxidant, and cytotoxic activities of the produced bio-oil. Tests were conducted using Gas Chromatography coupled with Mass Spectrometry (GC-MS) and assays to evaluate antimicrobial, antioxidant, and cytotoxic activities at different temperatures (350, 400, and 450 °C) and molarities (0.5 M, 1.0 M, and 2.0 M). Phenolic compounds were the most abundant in the bio-oil (55.70%), followed by cyclic and aromatic hydrocarbons (11.89%) and linear hydrocarbons (9.64%). Despite a reduction in oxygenated compounds, the bio-oil retained bacteriostatic activity against Escherichia coli and Staphylococcus aureus across various temperature ranges, with notable effectiveness at 350 °C. Antioxidant activity was highest at 350 °C and at lower molarities. Furthermore, lower concentrations of acidic impregnation exhibited cytotoxic effects at high temperatures. Thus, bio-oil from açaí seeds generated through pyrolysis shows potential for antioxidant and antimicrobial activities, suggesting feasibility for further testing in dilutions with lower cytotoxicity.Item Acesso aberto (Open Access) Efeitos citotóxicos e mecanismo de ação da eleuterina isolada de Eleutherine plicata em modelo in vitro de células c6(Universidade Federal do Pará, 2024-05) SHINKAI, Victória Mae Tsuruzaki; NASCIMENTO, José Luiz Martins do; http://lattes.cnpq.br/7216249286784978; https://orcid.org/0000-0003-3647-9124Glioblastoma multiforme (GBM) is the most prevalent malignant primary tumor of the central nervous system (CNS). GBM cells are characterized by rapid proliferation and aggressive migration. There is growing demand for new therapies to treat this tumor, due to current therapeutic limitations. Quinone derivatives from plants have received increased interest as potential antiglioma drugs due to their diverse pharmacological activities such as inhibition of cell growth, inflammation, tumor invasion and promotion of tumor regression. The herb Eleutherine plicata, popularly known as Marupazinho, is widely used in popular medicine due to its pharmacological properties, containing quinone derivatives, more specifically naphthoquinones. Previous studies have demonstrated the antiglioma activity of Eleutherine plicata, which is related to three main naphthoquinone compounds – eleutherine, isoeleutherine and eleutherol – but mechanism of action remains unclear. Thus, the objective of this study was to investigate the potential cytotoxic and antiproliferative effect of eleutherin in an in vitro model of glioblastoma (C6 lineage). In vitro cytotoxicity was assessed by the MTT assay; Morphological changes were assessed by phase contrast microscopy. Apoptosis was determined by the annexin V-FITCpropidium iodide assay, and antiproliferative effects were assessed by the colony formation assay. Protein kinase B (AKT/pAKT) expression was measured by western blot, and telomerase reverse transcriptase mRNA was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The results obtained indicated that eleutherin, isolated from the Hexanic fraction, had a cytotoxic effect on the C6 lineage. Structural changes were observed by image capture, with a significant reduction in colony formation, induction of apoptosis, inhibition of pAKT and reduction in telomerase expression after treatment. Thus, our study showed that the eleuterin molecule has cytotoxic activity in C6 lineage glioma.Item Acesso aberto (Open Access) Efeitos da Curcuma longa em modelo murino de acidente vascular cerebral(Universidade Federal do Pará, 2024-09) SANTOS, Vitória Corrêa; RÊGO, Dielly Catrina Favacho Lopes; http://lattes.cnpq.br/1810961422826950; https://orcid.org/0000-0002-6226-4269Stroke is the third leading cause of death and the main cause of functional impairment in adults. It can be hemorrhagic in nature, when a blood vessel in the brain ruptures, or ischemic, when there is obstruction of cerebral arterial blood flow. Ischemic stroke accounts for 87% of cases and is characterized by excitotoxicity, oxidative stress, neuroinflammation and cell death. Currently, treatment for ischemic stroke is limited to tissue plasminogen activator (tPA) therapy or mechanical thrombectomy, which makes the search for new pharmacological approaches crucial. In this scenario, Curcuma longa Linn (C. longa), known as turmeric, is a plant popularly used in cooking and traditional medicine and its main active compound is curcumin, responsible for giving C. longa anti-inflammatory, antioxidant, antimicrobial, antitumor, anticancer effects, among others. In the literature, C. longa has demonstrated promising activity against lesions caused by cerebral ischemia; however, the prolonged effects of the compound remain unknown. In this sense, this study evaluated the possible neuroprotective effects of C. longa in a murine model of transient focal cerebral ischemia. For that, 20 adult male Wistar rats (8 weeks old, weighing 300 ± 20 g; CEUA-UFPA no. 6868300622 [ID 001229]) underwent middle cerebral artery occlusion (MCAO) surgery for 30 minutes and treated with C. longa (MOTORE®) at a dose of 80 mg/kg every 12 hours for 14 days. The animals were divided into 4 groups (n = 4-5 animals per group): 1) Sham + V (animals with sham surgery that received vehicle [0.5 M NaOH + PBS]), 2) Sham + CL (animals with sham surgery that received C. longa), 3) MCAO + V (animals submitted to MCAO surgery that received vehicle) and 4) MCAO + CL (animals submitted to MCAO surgery that received C. longa). Metabolic parameters such as weight gain and water and food consumption were evaluated, as well as behavioral parameters through the neurological deficit score and the beam walking test, as well as histopathological parameters with the measurement of the infarct area and volume. In our results, no differences in body weight gain were found between the experimental groups. However, the sham + CL group consumed more water than the sham + V, MCAO + V and MCAO + CL groups, and the MCAO + CL group consumed less food on the 11th and 13th day after ischemia. Regarding behavioral deficits, both in the analysis of neurological deficit and in the beam walking test, the motor impairments evidenced by the MCAO + V and MCAO + CL groups were not attenuated by C.longa-treatment. Furthermore, treatment with C. longa did not attenuate the lesions caused by cerebral ischemia in our histological analyses. Thus, we conclude that treatment with Curcuma longa for 14 days did not exert a neuroprotective effect in the murine model of ischemic stroke, under our experimental conditions.