Teses em Doenças Tropicais (Doutorado) - PPGDT/NMT
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/3560
O Doutorado Acadêmico em Doenças Tropicais iniciou em 2007 e pertence ao Programa de Pós-Graduação em Doenças Tropicais do Núcleo de Medicina Tropical (NMT) da Universidade Federal do Pará (UFPA).
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Item Acesso aberto (Open Access) Caracterização da resposta imune citocínica na infecção humana pelo vírus oropouche e sua relação com o padrão de soroconversão e a presença de sintomas(Universidade Federal do Pará, 2011-12-19) OLIVEIRA, Euzébio de; VASCONCELOS, Pedro Fernando da Costa; http://lattes.cnpq.br/0973550817356564This thesis is the first global study that researches and analyzes the immune response of cytokine in human infections by Orthobunyavirus Oropuche virus. The study used 320 samples of human serum. Sixty were from the Blood Bank (negative control) and 260 were obtained from two outbreaks of the Oropouche virus in the State of Pará and Amapá (Brazil). The latter was divided into 8 subgroups for better data accuracy. The collected samples were analyzed for clinical data/symptoms with serologic testing by titration of antibodies by the hemagglutination inhibition (IgM/IgG) and the detection cytokines plasma levels by flow cytometry. This allowed for the technical description of cytokine. The data obtained allowed for the observation of the characteristics and the behavior of the cytokines signatures expressed by patients by the presence or not of the virus. This also allowed for the observation of changes to serum through the presence of specific symptoms such as fever, chills, headache and dizziness. This led to the following conclusions a) there is a pattern in the synthesis of pro-inflammatory and regulatory cytokines; b) there is a balance in the profile of the immune response between pro-inflammatory cytokines (Th1) and modulators (Th17); c) an infection by the Oropouche virus alters the production of cytokines in individuals; d) the results also show that whem comparing individuals no responders with early responders, there was an increase of IL-1β and decreased IL- 12; no responders with late responders, there was a decrease of IL-8, and increased IFN-α, IL-23 and IL-17; No responders occurred early responders compared with the increase IL-4 and IFN-g; However, when compared early responders and late responders, decreased IFN-α and IL-6; early responders generally showed a decrease in IL-10 and late responders showed an increase in IL-5; e) The results also show the expression of IL-5 in patients who showed symptoms specific for Oropouche infection (fever, chills, headache and dizziness), suggesting this signal to be directly associated with pathogenesis of the virus; f) there is a need to complement this research with more studies such as those related to the expression of chemokines.Item Acesso aberto (Open Access) Soroprevalência para arboviroses humanas de interesse em saúde pública como marcador de impacto ambiental em comunidades ribeirinhas que vivem sob a influência da usina hidrelétrica de Tucuruí-Pará(Universidade Federal do Pará, 2011) BRITO, Mioni Thieli Figueiredo Magalhães de; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054The arboviruses are a major public health problem in Brazil, especially in the Amazon for its ability to cause epidemics with considerable rates of morbidity and mortality in both humans and animals. Four, Virus dengue (VDEN), Virus yellow fever (VYF), Virus Mayaro (VMAY) and Virus Oropouche (VORO) has special relevance for the region, particularly in those environments where environmental impacts are imminent. Studies on the prevalence of antibodies to arboviruses in this region are limited. This study aimed to estimate the prevalence of antibodies to arboviruses of the major public health concern in the communities that live under the influence of the Tucuruí dam in the state of Pará. The study was observational, cross-sectional analytical study carried out in individuals of both sexes, aged over 18, resident at the left and right margin of the lake Tucuruí power plant and from the RDS and Alcobaça Pucurui-ararão. The collection of blood samples and filling in the questionnaire were performed at two different times, flood and ebb of the lake. All samples were analyzed by the Evandro Chagas Institute where they were tested with hemagglutination inhibition for detection of antibodies against 19 types of Arboviruses and MAC-ELISA test for detection of IgM antibodies. Data analysis was descriptive and analytical, are used to calculate odds ratio with confidence interval 95% to determine the association between the study variables and chi-square or Fisher exact test to verify the significance statistical relationships between the variables of the study with an alpha level of 0.05. In all, 635 individuals were studied and the antibodies to arboviruses was significantly associated with sociodemographic characteristics of individuals such as sex, ossupation and residence time in study area. No association was found between the presence of antibodies arboviral and other traits.